High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans

Applied and Environmental Microbiology

Volumn 78, Issue 13, 2012, Pages 4740-4743

  Lee, Siu F ^a   White, Vanessa L ^a   Weeks, Andrew R ^a   Hoffmann, Ary A ^a   Endersby, Nancy M ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

AEDES AEGYPTI; COST-EFFICIENT; HIGH-THROUGHPUT; PCR ASSAY;

POLYMERASE CHAIN REACTION; STRAIN;

BACTERIA;

BACTERIUM; BIOASSAY; DENGUE FEVER; DETECTION METHOD; FLUORESCENCE; FLY; HOST-PARASITE INTERACTION; MOSQUITO; PARASITE INFESTATION; POLYMERASE CHAIN REACTION;

AEDES; ANIMAL; ARTICLE; DROSOPHILA; ECONOMICS; FLUORESCENCE; GENETICS; HIGH THROUGHPUT SCREENING; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VALIDATION STUDY; WOLBACHIA;

AEDES; ANIMALS; DROSOPHILA; FLUORESCENCE; HIGH-THROUGHPUT SCREENING ASSAYS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; WOLBACHIA;

AEDES AEGYPTI; DROSOPHILA SIMULANS; WOLBACHIA;

EID: 84864085561     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00069-12     Document Type: Article

References (15)

1. Ballard JW. 2004. Sequential evolution of a symbiont inferred from the host: Wolbachia and Drosophila simulans. Mol. Biol. Evol. 21:428-442.
2. Braig HR, Zhou W, Dobson SL, O'Neill SL. 1998. Cloning and characterization of a gene encoding the major surface protein of the bacterial endosymbiont Wolbachia pipientis. J. Bacteriol. 180:2373-2378.
3. Caragata EP, et al. 2011. Improved accuracy of the transcriptional profiling method of age grading in Aedes aegypti mosquitoes under laboratory and semi-field cage conditions and in the presence of Wolbachia infection. Insect Mol. Biol. 20:215-224.
4. Hoffmann AA, Clancy D, Duncan J. 1996. Naturally-occurring Wolbachia infection in Drosophila simulans that does not cause cytoplasmic incompatibility. Heredity 76:1-8.
5. Hoffmann AA, et al. 2011. Successful establishment of Wolbachia in Aedes populations to suppress dengue transmission. Nature 476:454-457.
6. Hoffmann AA, Turelli M, Simmons GM. 1986. Unidirectional incompatibility between populations of Drosophila simulans. Evolution 40:692-701.
7. Holden PR, Brookfield JF, Jones P. 1993. Cloning and characterization of an ftsZ homologue from a bacterial symbiont of Drosophila melanogaster. Mol. Gen. Genet. 240:213-220.
8. O'Neill SL, Giordano R, Colbert AM, Karr TL, Robertson HM. 1992. 16S rRNA phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects. Proc. Natl. Acad. Sci. U. S. A. 89:2699-2702.
9. Riegler M, Sidhu M, Miller WJ, O'Neill SL. 2005. Evidence for a global Wolbachia replacement in Drosophila melanogaster. Curr. Biol. 15:1428-1433.
10. Turelli M, Hoffmann AA. 1999. Microbe-induced cytoplasmic incompatibility as a mechanism for introducing transgenes into arthropod populations. Insect Mol. Biol. 8:243.
11. Turelli M, Hoffmann AA. 1991. Rapid spread of an inherited incompatibility factor in California Drosophila. Nature 353:440-442.
12. Walker T, et al. 2011. The wMel Wolbachia strain blocks dengue and invades caged Aedes aegypti populations. Nature 476:450-453.
13. Werren JH, Windsor DM. 2000. Wolbachia infection frequencies in insects: evidence of a global equilibrium? Proc. Biol. Sci. 267:1277-1285.
14. Werren JH, Zhang W, Guo LR. 1995. Evolution and phylogeny of Wolbachia: reproductive parasites of arthropods. Proc. Biol. Sci. 261:55-63.
15. Zhou W, Rousset F, O'Neill S. 1998. Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences. Proc. Biol. Sci. 265: 509-515