Molecular diagnosis of polymicrobial newborn sepsis by multiplex real-time PCR using a small volume of blood sample

Journal of Medical Microbiology

Volumn 61, Issue 8, 2012, Pages 1177-1178

  Vrioni, Georgia ^a   Daniil, Ioannis ^b   Drogari Apiranthitou, Miranda ^a   Kimouli, Maria ^b   Papadopoulou, Magdalini ^b   Tsakris, Athanassios ^a  

^a UNIVERSITY OF ATHENS MEDICAL SCHOOL   (Greece)

^b Saint Panteleimon General Hospital   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

AMPHOTERICIN B; ANTIBIOTIC AGENT; ANTIFUNGAL AGENT; CIPROFLOXACIN; FLUCYTOSINE; NETILMICIN; TEICOPLANIN;

ARTICLE; BLOOD CULTURE; BLOOD SAMPLING; BLOOD VOLUME; BLOODSTREAM INFECTION; CANDIDA ALBICANS; CANDIDEMIA; CANDIDIASIS; EARLY DIAGNOSIS; ESCHERICHIA COLI; FUNGUS GROWTH; MOLECULAR DIAGNOSIS; MULTIPLEX POLYMERASE CHAIN REACTION; MYCOSIS; NEWBORN MORBIDITY; NEWBORN MORTALITY; NEWBORN SEPSIS; POLYMICROBIAL NEWBORN SEPSIS; PREMATURITY; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RECTUM; STENOTROPHOMONAS MALTOPHILIA; VERY LOW BIRTH WEIGHT;

BACTERIOLOGICAL TECHNIQUES; BLOOD; COINFECTION; HUMANS; INFANT, NEWBORN; MALE; MOLECULAR DIAGNOSTIC TECHNIQUES; MULTIPLEX POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; SEPSIS;

EID: 84864080835     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.037127-0     Document Type: Article

References (11)

1. Gaibani, P., Rossini, G., Ambretti, S., Gelsomino, F., Pierro, A. M., Varani, S., Paolucci, M., Landini, M. P. and Sambri, V. (2009). Blood culture systems: rapid detection - how and why? Int J Antimicrob Agents 34 (Suppl. 4), S13-S15.
2. Kaufman, D. and Fairchild, K. D. (2004). Clinical microbiology of bacterial and fungal sepsis in very-low-birth-weight infants. Clin Microbiol Rev 17, 638-680.
3. Lehmann, L. E., Hunfeld, K. P., Emrich, T., Haberhausen, G., Wissing, H., Hoeft, A. and Stuber, F. (2008). A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med Microbiol Immunol (Berl) 197, 313-324.
4. Lott, J. W. (2003). Neonatal bacterial sepsis. Crit Care Nurs Clin North Am 15, 35-46.
5. Lucignano, B., Ranno, S., Liesenfeld, O., Pizzorno, B., Putignani, L., Bernaschi, P. and Menichella, D. (2011). Multiplex PCR allows rapid and accurate diagnosis of bloodstream infections in newborns and children with suspected sepsis. J Clin Microbiol 49, 2252-2258.
6. Mancini, N., Carletti, S., Ghidoli, N., Cichero, P., Ossi, C. M., Ieri, R., Poli, E., Burioni, R. and Clementi, M. (2009). Molecular diagnosis of polymicrobial sepsis. J Clin Microbiol 47, 1274-1275.
7. Mussap, M., Molinari, M. P., Senno, E., Gritti, P., Soro, B., Mannelli, S. and Fabris, C. (2007). New diagnostic tools for neonatal sepsis: the role of a real-time polymerase chain reaction for the early detection and identification of bacterial and fungal species in blood samples. J Chemother 19 (Suppl. 2), 31-34.
8. Ostrosky-Zeichner, L. and Pappas, P. G. (2006). Invasive candidiasis in the intensive care unit. Crit Care Med 34, 857-863.
9. Paolucci, M., Capretti, M. G., Dal Monte, P., Corvaglia, L., Landini, M. P., Varani, S., Pession, A., Faldella, G. and Sambri, V. (2009). Laboratory diagnosis of late-onset sepsis in newborns by multiplex real-time PCR. J Med Microbiol 58, 533-534.
10. Peters, R. P., van Agtmael, M. A., Danner, S. A., Savelkoul, P. H. and Vandenbroucke-Grauls, C. M. (2004). New developments in the diagnosis of bloodstream infections. Lancet Infect Dis 4, 751-760.
11. von Lilienfeld-Toal, M., Lehmann, L. E., Raadts, A. D., Hahn-Ast, C., Orlopp, K. S., Marklein, G., Purr, I., Cook, G., Hoeft, A. and other authors (2009). Utility of a commercially available multiplex real-time PCR assay to detect bacterial and fungal pathogens in febrile neutropenia. J Clin Microbiol 47, 2405-2410.