Biologically active, magnICON®-expressed EPO-Fc from stably transformed Nicotiana benthamiana plants presenting tetra-antennary N-glycan structures

Journal of Biotechnology

Volumn 160, Issue 3-4, 2012, Pages 242-250

  Nagels, Bieke ^a,b   Van Damme, Els J M ^a   Callewaert, Nico ^a,c   Zabeau, Lennart ^c   Tavernier, Jan ^c   Delanghe, Joris R ^d   Boets, Annemie ^b   Castilho, Alexandra ^e   Weterings, Koen ^b,f  

^a GHENT UNIVERSITY   (Belgium)

^b Bayer sa nv Belgium   (Belgium)

^c DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^d GHENT UNIVERSITY HOSPITAL   (Belgium)

^e UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^f BAYER CROPSCIENCE   (United States)

Author keywords

EPOR; Erythropoietin; FucT; GnT; In vitro activity assay; LC ESI MS; LR; Molecular farming; N glycosylation; Nicotiana benthamiana; RhEPO; XylT

Indexed keywords

EPOR; ERYTHROPOIETIN (EPO); FUCT; GNT; IN-VITRO; LC-ESI MS; MOLECULAR FARMING; N-GLYCOSYLATION; NICOTIANA BENTHAMIANA; RHEPO; XYLT;

ESTERIFICATION; GLYCOSYLATION; LAWRENCIUM; MASS SPECTROMETRY; PARAMAGNETISM; PRODUCTION PLATFORMS;

PROTEINS;

ALPHA 1,3 FUCOSE; BETA 1,2 XYLOSE; ERYTHROPOIETIN; FUCOSE; GLYCOPEPTIDASE; HYBRID PROTEIN; IMMUNOGLOBULIN G; RECOMBINANT ERYTHROPOIETIN; UNCLASSIFIED DRUG; VEGETABLE PROTEIN; XYLOSE;

ARTICLE; GENE EXPRESSION SYSTEM; IMMUNOASSAY; IN VITRO STUDY; MASS SPECTROMETRY; NICOTIANA BENTHAMIANA; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN STRUCTURE;

ERYTHROPOIETIN; IMMUNOGLOBULIN FRAGMENTS; PLANTS, GENETICALLY MODIFIED; POLYSACCHARIDES; PROTEIN ENGINEERING; TOBACCO; TRANSFORMATION, GENETIC;

NICOTIANA BENTHAMIANA; TETRA;

EID: 84864066473     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2012.03.003     Document Type: Article

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