Differential staining of glia and neurons by modified Golgi-Cox method

Journal of Neuroscience Methods

Volumn 209, Issue 2, 2012, Pages 269-279

  Ranjan, Amit ^a   Mallick, Birendra Nath ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

Author keywords

Differential staining of neurons and glia; Fixative; Golgi Cox stain; Incubation temperature

Indexed keywords

PARAFORMALDEHYDE; SUCROSE;

ANIMAL TISSUE; ARTICLE; BRAIN; CELL COUNT; CELL STRUCTURE; GLIA CELL; GOLGI STAIN; HISTOLOGY; INCUBATION TEMPERATURE; MALE; MEMBRANE DAMAGE; MICROGLIA; NERVE CELL; NONHUMAN; PH; PRIORITY JOURNAL; RAT;

ANIMALS; BRAIN; HYDROGEN-ION CONCENTRATION; MALE; NEUROGLIA; NEURONS; RATS; RATS, WISTAR; SILVER STAINING; SUCROSE; TEMPERATURE; TISSUE FIXATION;

EID: 84864047515     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2012.06.023     Document Type: Article

References (41)

1. Adams J.C. A fast, reliable silver-chromate Golgi method for perfusion-fixed tissue. Stain Technol 1979, 54:225-226.
2. Angulo A., Merchan J.A., Molina M. Golgi-Colonnier method: correlation of the degree of chromium reduction and pH change with quality of staining. J Histochem Cytochem 1994, 42:393-403.
3. Armstrong E., Parker B. A new Golgi method for adult human brains. J Neurosci Methods 1986, 17:247-254.
4. Berbel P.J. Chromation at low temperatures improves impregnation of neurons in Golgi-aldehyde methods. J Neurosci Methods 1986, 17:255-259.
5. Bolton J.S. A preliminary note on the Golgi impregnation of formalin-hardened brain. Br Med J 1898, 1:367.
6. Buell S.J. Golgi-Cox and rapid golgi methods as applied to autopsied human brain tissue: widely disparate results. J Neuropathol Exp Neurol 1982, 41:500-507.
7. D'Amelio F.E. The Golgi-Hortega-Lavilla technique, with a useful additional step for application to brain tissue after prolonged fixation. Stain Technol 1983, 58:79-84.
8. Dall'Oglio A., Ferme D., Brusco J., Moreira J.E., The Rasia-Filho A.A. single-section Golgi method adapted for formalin-fixed human brain and light microscopy. J Neurosci Methods 2010, 189:51-55.
9. Fox C.H., Johnson F.B., Whiting J., Roller P.P. Formaldehyde fixation. J Histochem Cytochem 1985, 33:845-853.
10. Friedland D.R., Los J.G., Ryugo D.K. A modified Golgi staining protocol for use in the human brain stem and cerebellum. J Neurosci Methods 2006, 150:90-95.
11. Garcia-Marin V., Garcia-Lopez P., Freire M. Cajal's contributions to glia research. Trends Neurosci 2007, 30:479-487.
12. Gibb R., Kolb B. A method for vibratome sectioning of Golgi-Cox stained whole rat brain. J Neurosci Methods 1998, 79:1-4.
13. Glaser E.M., Van der Loos H. Analysis of thick brain sections by obverse-reverse computer microscopy: application of a new, high clarity Golgi-Nissl stain. J Neurosci Methods 1981, 4:117-125.
14. Globus A., Scheibel A.B. Loss of dendrite spines as an index of pre-synaptic terminal patterns. Nature 1966, 212:463-465.
15. Howard M.J., Barnes P.R. A silver carbonate method for cell counts of neurons and glial elements on paraffin embedded brain tissue. Stain Technol 1979, 54:299-303.
16. Ibrahim M.Z., Call N., Noden P. Modifications of the Hortega silver carbonate method adapted for celloidin-embedded and frozen sections. Acta Neuropathol 1968, 10:258-260.
17. Kaneko R., Sueoka N. Tissue-specific versus cell type-specific expression of the glial fibrillary acidic protein. Proc Natl Acad Sci U S A 1993, 90:4698-4702.
18. Klatzo I. A study of glia by the Golgi method. Lab Invest 1952, 1:345-350.
19. Kuner T., Augustine G.J. A genetically encoded ratiometric indicator for chloride: capturing chloride transients in cultured hippocampal neurons. Neuron 2000, 27:447-459.
20. Landas S., Phillips M.I. Staining of human and rat brain Vibratome sections by a new Golgi method. J Neurosci Methods 1982, 5:147-151.
21. Marani E., Guldemond J.M., Adriolo P.J., Boon M.E., Kok L.P. The microwave Rio-Hortega technique: a 24h method. Histochem J 1987, 19:658-664.
22. Marin-Padilla M. Prenatal development of fibrous (white matter), protoplasmic (gray matter), and layer I astrocytes in the human cerebral cortex: a Golgi study. J Comp Neurol 1995, 357:554-572.
23. Marin-Padilla M. Three-dimensional structural organization of layer I of the human cerebral cortex: a Golgi study. J Comp Neurol 1990, 299:89-105.
24. Millhouse O.E. A Golgi study of the desending medial forebrain bundle. Brain Res 1969, 15:341-363.
25. Moliner E.R. A chlorate-formaldehyde modification of the Golgi method. Stain Technol 1957, 32:105-116.
26. Mullen R.J., Buck C.R., Smith A.M. NeuN, a neuronal specific nuclear protein in vertebrates. Development 1992, 116:201-211.
27. Pessacq T.P. A chloral hydrate modification of the Golgi-Cox method for impregnation of glia cells. Acta Anat (Basel) 1970, 76:131-135.
28. Rakic P. Neuron-glia relationship during granule cell migration in developing cerebellar cortex. A Golgi and electronmicroscopic study in Macacus Rhesus. J Comp Neurol 1971, 141:283-312.
29. Ranjan A., Mallick B.N. A modified method for consistent and reliable Golgi-cox staining in significantly reduced time. Front Neurol 2010, 1:157.
30. Rosoklija G., Mancevski B., Ilievski B., Perera T., Lisanby S.H., Coplan J.D., et al. Optimization of Golgi methods for impregnation of brain tissue from humans and monkeys. J Neurosci Methods 2003, 131:1-7.
31. Rutledge L.T., Duncan J., Beatty N. A study of pyramidal cell axon collaterals in intact and partially isolated adult cerebral cortex. Brain Res 1969, 16:15-22.
32. Schmechel D., Marangos P.J., Zis A.P., Brightman M., Goodwin F.K. Brain endolases as specific markers of neuronal and glial cells. Science 1978, 199:313-315.
33. Silva-Gomez A.B., Rojas D., Juarez I., Flores G. Decreased dendritic spine density on prefrontal cortical and hippocampal pyramidal neurons in postweaning social isolation rats. Brain Res 2003, 983:128-136.
34. Silver I.A., Deas J., Erecinska M. Ion homeostasis in brain cells: differences in intracellular ion responses to energy limitation between cultured neurons and glial cells. Neuroscience 1997, 78:589-601.
35. Smith Q.R., Johanson C.E., Woodbury D.M. Uptake of 36Cl and 22Na by the brain-cerebrospinal fluid system: comparison of the permeability of the blood-brain and blood-cerebrospinal fluid barriers. J Neurochem 1981, 37:117-124.
36. Spacek J. Dynamics of the Golgi method: a time-lapse study of the early stages of impregnation in single sections. J Neurocytol 1989, 18:27-38.
37. Stensaas L.J., Stensaas S.S. Astrocytic neuroglial cells, oligodendrocytes and microgliacytes in the spinal cord of the toad. I. Light microscopy. Z Zellforsch Mikrosk Anat 1968, 84:473-489.
38. Stensaas L.J., Stensaas S.S. Astrocytic neuroglial cells, oligodendrocytes and microgliacytes in the spinal cord of the toad. II. Electron microscopy. Z Zellforsch Mikrosk Anat 1968, 86:184-213.
39. Williams R.S. Golgi's method of staining nerve cells. J Neuropathol Exp Neurol 1983, 42:210-212.
40. Williams R.S., Ferrante R.J., Caviness V.S. The Golgi rapid method in clinical neuropathology: the morphologic consequences of suboptimal fixation. J Neuropathol Exp Neurol 1978, 37:13-33.
41. Zhang H., Weng S.J., Hutsler J.J. Does microwaving enhance the Golgi methods? A quantitative analysis of disparate staining patterns in the cerebral cortex. J Neurosci Methods 2003, 124:145-155.