Comparison of expression and enzymatic properties of Aspergillus oryzae lysine aminopeptidases ApsA and ApsB

World Journal of Microbiology and Biotechnology

Volumn 28, Issue 8, 2012, Pages 2643-2650

  Marui, Junichiro ^a,e   Matsushita Morita, Mayumi ^a   Tada, Sawaki ^a   Hattori, Ryota ^a   Suzuki, Satoshi ^a   Amano, Hitoshi ^b   Ishida, Hiroki ^c   Yamagata, Youhei ^d   Takeuchi, Michio ^d   Kusumoto, Ken Ichi ^a  

^a NATIONAL FOOD RESEARCH INSTITUTE   (Japan)

^b Amano Enzyme Inc   (Japan)

^c Gekkeikan Sake Company Ltd   (Japan)

^d TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

^e JAPAN INTERNATIONAL RESEARCH CENTER FOR AGRICULTURAL SCIENCES   (Japan)

Author keywords

Aspergillus oryzae; Family M1 aminopeptidase; Lysine; Peptide chain length dependent reactivity

Indexed keywords

AMINO PEPTIDASE; ANIMAL FEED; ASPERGILLUS ORYZAE; CARBON SOURCE; CATALYTIC MOTIF; ENCODED PROTEINS; ENZYMATIC PROPERTIES; ESSENTIAL AMINO ACIDS; GENES ENCODING; GROWTH MEDIA; LYSINE; N-TERMINALS; PEPTIDE CHAIN; PEPTIDE SUBSTRATES; PH RANGE; SUBSTRATE SPECIFICITY; UP-REGULATION;

ASPERGILLUS; ENZYMES; GENES; PEPTIDES;

AMINO ACIDS;

AMINOPEPTIDASE; FUNGAL PROTEIN; ISOENZYME; LYSYL AMINOPEPTIDASE; RECOMBINANT PROTEIN;

ARTICLE; ASPERGILLUS ORYZAE; CHEMISTRY; COMPARATIVE STUDY; ENZYME SPECIFICITY; ENZYMOLOGY; FUNGAL GENE; GENE EXPRESSION REGULATION; GENETICS; METABOLISM;

AMINOPEPTIDASES; ASPERGILLUS ORYZAE; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; ISOENZYMES; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

ANIMALIA; ASPERGILLUS ORYZAE; FUNGI;

EID: 84864030008     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-012-1074-6     Document Type: Article

References (16)

1. Basten DE, Visser J, Schaap PJ (2001) Lysine aminopeptidase of Aspergillus niger. Microbiology 147: 2045-2050.
2. Blinkovsky AM, Byun T, Brown KM, Golightly EJ, Klotz AV (2000) A non-specific aminopeptidase from Aspergillus. Biochim Biophys Acta 1480: 171-181.
3. Gonzales T, Robert-Baudouy J (1996) Bacterial aminopeptidases: properties and functions. FEMS Microbiol Rev 18: 319-344.
4. Kobayashi T, Abe K, Asai K, Gomi K, Juvvadi P, Kato M, Kitamoto K, Takeuchi M, Machida M (2007) Genomics of Aspergillus oryzae. Biosci Biotechnol Biochem 71: 646-670.
5. Kusumoto K, Matsushita-Morita M, Furukawa I, Suzuki S, Yamagata Y, Koide Y, Ishida H, Takeuchi M, Kashiwagi Y (2008) Efficient production and partial characterization of aspartyl aminopeptidase from Aspergillus oryzae. J Appl Microbiol 105: 1711-1719.
6. Machida M, Asai K, Sano M, Tanaka T, Kumagai T, Terai G, Kusumoto K, Arima T, Akita O et al (2005) Genome sequencing and analysis of Aspergillus oryzae. Nature 438: 1157-1161.
7. Machida M, Yamada O, Gomi K (2008) Genomics of Aspergillus oryzae: learning from the history of Koji mold and exploration of its future. DNA Res 15: 173-183.
8. Marui J, Matsushita-Morita M, Tada S, Hattori R, Suzuki S, Amano H, Ishida H, Yamagata Y, Takeuchi M, Kusumoto K (2012) Enzymatic properties of the glycine d-alanine aminopeptidase of Aspergillus oryzae and its activity profiles in liquid-cultured mycelia and solid-state rice culture (rice koji). Appl Microbiol Biotechnol 93: 655-669.
9. Matsushita-Morita M, Furukawa I, Suzuki S, Yamagata Y, Koide Y, Ishida H, Takeuchi M, Kashiwagi Y, Kusumoto K (2010) Characterization of recombinant prolyl aminopeptidase from Aspergillus oryzae. J Appl Microbiol 109: 156-165.
10. Matsushita-Morita M, Tada S, Suzuki S, Hattori R, Marui J, Furukawa I, Yamagata Y, Amano H, Ishida H, Takeuchi M, Kusumoto K (2011) Overexpression and characterization of an extracellular leucine aminopeptidase from Aspergillus oryzae. Curr Microbiol 62: 557-564.
11. Mintz GR (1993) An irreversible serine protease inhib. Biopharm 6: 34-38.
12. Powers JC, Asgian JA, Ekici ÖD, James KE (2002) Irreversible inhibitors of serine, cysteine, and threonine proteases. Chem Rev 102: 4639-4750.
13. Rawlings ND, Barrett AJ (2004) Aspartic and metallo peptidases. In: Barrett AJ, Rawlings ND, Woessner JF (eds) Handbook of proteolytic enzymes, 2nd edn. Elsevier, Amsterdam, pp 231-268.
14. Rawlings ND, Barrett AJ, Bateman A (2010) MEROPS: the peptidase database. Nucleic Acids Res 38: D227-D233.
15. Taylor A (1993) Aminopeptidases: structure and function. FASEB J 7: 290-298.
16. Young VR, Pellett PL (1994) Plant proteins in relation to human protein and amino acid nutrition. Am J Clin Nutr 59: 1203S-1212S.