Enhanced fungal DNA-extraction from formalin-fixed, paraffin-embedded tissue specimens by application of thermal energy

Medical Mycology

Volumn 50, Issue 6, 2012, Pages 667-672

  Rickerts, Volker ^a   Khot, Prasanna D ^b   Ko, Daisy L ^b   Fredricks, David N ^b  

^a ROBERT KOCH INSTITUTE   (Germany)

^b USA   (United States)

Author keywords

Aspergillosis; Candidiasis; DNA extraction; Formalin fixed tissue specimens; PCR

Indexed keywords

FORMALDEHYDE; FUNGAL DNA; PARAFFIN;

ARTICLE; ASPERGILLUS FLAVUS; ASPERGILLUS FUMIGATUS; CANDIDA ALBICANS; CANDIDA TROPICALIS; CRYPTOCOCCUS NEOFORMANS; DNA EXTRACTION; FUNGUS IDENTIFICATION; GENE AMPLIFICATION; HUMAN; HUMAN TISSUE; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; SYSTEMIC MYCOSIS; AMPLICON; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC KIT; DNA DETERMINATION; ENERGY; FUNGAL STRAIN; HISTOPATHOLOGY; INCUBATION TEMPERATURE; INCUBATION TIME; SEQUENCE ANALYSIS; SPECIES IDENTIFICATION; THERMAL ENERGY; TISSUE FIXATION; TISSUE SECTION;

ASPERGILLOSIS; ASPERGILLUS; CANDIDA; DNA, FUNGAL; FORMALDEHYDE; GENOME, HUMAN; HOT TEMPERATURE; HUMANS; LUNG; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PARAFFIN EMBEDDING; PATHOLOGY, MOLECULAR; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TISSUE FIXATION;

FUNGI;

EID: 84863899273     PISSN: 13693786     EISSN: 14602709     Source Type: Journal    
DOI: 10.3109/13693786.2012.665613     Document Type: Article

References (13)

1. Chamilos G, Luna M, Lewis RE, e t al . Invasive fungal infections in patients with hematologic malignancies in a tertiary care cancer center: An autopsy study over a 15 year period (1989-2003). Haematologica 2006; 91: 986-989.
2. Rickerts V, Mousst S, Lambrecht E, et al . Comparison of histopathological analysis, culture, and polymerase chain reaction assays to detect invasive mold infections from biopsy specimens. Clin Infect Dis 2007; 44: 1078-1083.
3. Willinger BA, Obradovic A, Selitsch B, et al . Detection and identifi - cation of fungi from fungus balls of the maxillary sinus by molecular techniques. J Clin Microbiol 2003; 41: 581-585.
4. Lau AS, Chen S, Sorrell T, et al . Development and clinical application of a panfungal PCR assay to detect and identify fungal DNA in tissue specimens. J Clin Microbiol 2007; 45: 380-385.
5. Gilbert MT, Haselkorn T, Bunce M, e t al . The isolation of nucleic acids from fixed, paraffi n-Embedded tissues-which methods are useful when. Plos One 2007; 2: E537.
6. Rickerts V, Khot PD, Myerson D, et al . Comparison of quantitative real time PCR with sequencing and ribosomal RNA-FISH for the identifi cation of fungi in Formalin fixed, paraffi n-Embedded tissue specimens. BMC Infect Dis 2011; 11: 202.
7. Khot PD, Ko DL, Hackman RC, et al . Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid. BMC Infect Dis 2008; 8: 73.
8. Khot PD, Ko DL, Fredricks DN. Sequencing and analysis of fungal rRNA operons for development of broad-Range fungal PCR assays. Appl Environ Microbiol 2009; 75: 1559-1565.
9. Munoz-Cadavid C, Rudd S, Zaki SR, e t al . Improving molecular detection for fungal DNA in formalin-Fixed paraffi n-Embedded tissues: Comparison of fi ve tissue DNA extraction methods using panfungal PCR. J Clin Microbiol 2010; 48: 2147-2153.
10. Cabaret O, Toussain G, Abermil N, et al . Degradation of fungal DNA in formalin-Fixed paraffi n embedded sinus fungal balls hampers reliable sequence-Based identifi cation of fungi. Med Mycol 2011; 49: 329-332.
11. Khot PD, Fredricks DN. PCR-Based diagnosis of human fungal infections. Expert Rev Anti Infect Ther 2009; 7: 1201-1221.
12. Bialek R, Konrad F, Kern J, et al . PCR based identification and discrimination of mucormycosis and aspergillosis in paraffin wax embedded tissue. J Clin Pathol 2005; 58: 1180-1184.
13. Dannaoui EP, Schwarz, Slany M, e t al . Molecular detection and identifi cation of zygomycetes species from paraffi n-Embedded tissues in a murine model of disseminated zygomycosis: A collaborative European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Fungal Infection Study Group (EFISG) evaluation. J Clin Microbiol 2010; 48: 2043-2046.