Efficient and accurate analysis of mitochondrial morphology in a whole cell with a high-voltage electron microscopy

Journal of Electron Microscopy

Volumn 61, Issue 2, 2012, Pages 127-131

  Kim, Hyun Wook ^a   Oh, Seung Hak ^a   Kim, Ji Woong ^a   Cho, Bongki ^a   Park, In Sung ^a   Sun, Woong ^a   Rhyu, Im Joo ^a  

^a Korea University College of Medicine   (South Korea)

Author keywords

HVEM; Mitochondria; Morphology

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN;

ANIMAL; ARTICLE; CELL CULTURE; CELL STRAIN COS1; CERCOPITHECUS; GENETICS; HELA CELL; HUMAN; METABOLISM; METHODOLOGY; MITOCHONDRION; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

ANIMALS; CELLS, CULTURED; CERCOPITHECUS AETHIOPS; COS CELLS; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; MICROSCOPY, ELECTRON, TRANSMISSION; MITOCHONDRIA;

EUKARYOTA;

ADENOSINETRIPHOSPHATE; ELECTRON MICROSCOPES; ELECTRON MICROSCOPY; MITOCHONDRIA;

ACCURATE ANALYSIS; ADENOSINE TRIPHOSPHATE; CALCIUM HOMEOSTASIS; CELLULAR CONDITIONS; CULTURED CELL; EFFICIENT ANALYSIS; HIGH VOLTAGE ELECTRON MICROSCOPY; MITOCHONDRIAL MORPHOLOGY; MITOCHONDRIAS; WHOLE CELL;

MORPHOLOGY;

EID: 84863894710     PISSN: 00220744     EISSN: 14779986     Source Type: Journal    
DOI: 10.1093/jmicro/dfs001     Document Type: Article

References (12)

1. Detmer S A and Chan D C (2007) Functions and dysfunctions of mitochondrial dynamics. Nat. Rev. Mol. Cell Biol. 8: 870-879. (Pubitemid 47622562)
2. Soubannier V and Mcbride H M (2009) Positioning mitochondrial plasticity within cellular signaling cascades. Biochim. Biophys. Acta 1793: 154-170.
3. Westermann B (2010) Mitochondrial fusion and fission in cell life and death. Nat. Rev. Mol. Cell Biol. 11: 872-884.
4. Chan D C (2006) Mitochondrial fusion and fission in mammals. Annu. Rev. Cell Dev. Biol. 22: 79-99.
5. Chen H and Chan D C (2009) Mitochondrial dynamics-fusion, fission, movement, and mitophagy-in neurodegenerative diseases. Hum. Mol. Genet. 18: R169-R176.
6. Glauert A M (1974) The high voltage electron microscope in biology. J. Cell Biol. 63: 717-748.
7. Hama K (1989) Biological application of high voltage electron mi croscopy. J. Electron. Microsc. (Tokyo) 38 (Suppl): S156-S162.
8. Nagata T (2001) Three-dimensional high voltage electron microscopy of thick biological specimens. Micron 32: 387-404. (Pubitemid 32062528)
9. Ruska E (1987) Nobel lecture: the development of the electron microscope and of electron microscopy. Biosci. Rep. 7: 607-629.
10. Takaoka A, Hasegawa T, Yoshida K, and Mori H (2008) Microscopic tomography with ultra-HVEM and applications. Ultramicroscopy 108: 230-238.
11. Goldstein S, Moerman E J, and Porter K (1984) High-voltage electron microscopy of human diploid fibroblasts during ageing in vitro: mor-phometric analysis of mitochondria. Exp. Cell Res. 154: 101-111. (Pubitemid 14006412)
12. Noske A B, Costin A J, Morgan G P, and Marsh B J (2008) Expedited approaches to whole cell electron tomography and organelle mark-up in situ in high-pressure frozen pancreatic islets. J. Struct. Biol. 161: 298-313.