Isolation, genome phylogenetic analysis and in vitro rescue of a newly emerging porcine circovirus type 2

Pakistan Veterinary Journal

Volumn 32, Issue 2, 2012, Pages 165-170

  Zhu, Weijuan ^a   Ren, Xiaofeng ^a  

^a NORTHEAST AGRICULTURAL UNIVERSITY   (China)

Author keywords

Genome; Porcine circovirus type 2; Virus isolation; Virus rescue

Indexed keywords

CIRCOVIRUS; PORCINE CIRCOVIRUS 2;

EID: 84863882339     PISSN: 02538318     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (15)

1. Allemandou A, B Grasland, AC Hernandez-Nignol, A Kéranflec'h, R Cariolet and A Jestin, 2011. Modification of PCV-2 virulence by substitution of the genogroup motif of the capsid protein. Vet Res, 42: 54.
2. Calsamiglia M, J Segalés, J Quintana, C Rosell and M Domingo, 2002.Detection of porcine circovirus types 1 and 2 in serum and tissue samples of pigs with and without postweaning multisystemic wasting syndrome. J Clin Microbiol, 40: 1848-1850.
3. Chen HC, ST Chiou, JY Zheng, SH Yang, SS Lai and TY Kuo, 2011. The nuclear localization signal sequence of porcine circovirus type 2 ORF2 enhances intracellular delivery of plasmid DNA. Arch Virol,156: 803-815.
4. Dupont K, EO Nielsen, P Baekbo and LE Larsen, 2008. Genetic analysis of PCV2 isolates from Danish archives and a current PMW Scase control study supports a shift in genotypes with time. Vet Microbiol, 128: 56-64.
5. Fenaux M, PG Halbur, G Haqshenas, R Royer, P Thomas, P Nawagitgul,M Gill, TE Toth and XJ Meng, 2002. Cloned genomic DNA of type2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: characterization of clinical disease,virus distribution, and pathologic lesions. J Virol, 76: 541-551.
6. Fu F, X Li, Y Lang, Y Yang, G Tong, G Li, Y Zhou and X Li, 2011. Coexpression of ubiquitin gene and capsid protein gene enhances the potency of DNA immunization of PCV2 in mice. Virol J, 8: 264.
7. Harding JC, JA Ellis, KA McIntosh and S Krakowka, 2010. Dual heterologous porcine circovirus genogroup 2a/2b infection induces severe disease in germ-free pigs. Vet Microbiol, 145: 209-219.
8. Ren XF, JC Yin, DX Ma and GX Li, 2009. Characterization and membrane gene-based phylogenetic analysis of avian infectious bronchitis virus Chinese strain HH06. Virus Genes, 38: 39-45.
9. Rosell C, J Segale's, JA Ramos-Vara, JM Folch, GM Rodrl'guez-Arrioja, CO Duran, M Balasch, J Plana-Dura'n and M Domingo, 2000.Identification of porcine circovirus in tissues of pigs with porcine dermatitis and nephropathy syndrome. Vet Rec, 146: 40-43.
10. Shang SB, YL Jin, XT Jiang, JY Zhou, X Zhang, G Xing, JL He and Y Yan,2009. Fine mapping of antigenic epitopes on capsid proteins of porcine circovirus and antigenic phenotype of porcine circovirus type2. Mol Immunol, 46: 327-334.
11. Shuai J, L Fu, X Zhang, B Zhu, X Li, Y He and W Fang, 2011. Functional exchangeability of the nuclear localization signal (NLS) of capsid protein between PCV1and PCV2 in vitro: Implications for the role of NLS in viral replication. Virol J, 8: 341.
12. Sinha A, HG Shen, S Schalk, NM Beach, YW Huang, PG Halbur, XJ Meng and T Opriessnig, 2010. Porcine reproductive and respiratory syndrome virus infection at the time of porcine circovirus type2 vaccination has no impact on vaccine efficacy. Clin Vaccine Immunol, 17: 1940-1945.
13. Todd D, JH Weston, D Soike and JA Smyth, 2001. Genome sequence determinations and analyses of novel circovirus from goose and pigeon. Virology, 286: 354-362.
14. Wellenberg GJ, N Stockhofe-Zurwieden, WJA Boersma, MF de Jong and ARW Elbers, 2004. The presence of co-infections in pigs with clinical signs of PMWS in the Netherlands: a case-control study.Res Vet Sci, 77: 177-184.
15. Yi JZ and CQ Liu, 2010. Molecular characterization of porcine circovirus 2 isolated from diseased pigs co-infected with porcine reproductive and respiratory syndrome virus. Virol J, 7: 286.