TRI12 based quantitative real-time PCR assays reveal the distribution of trichothecene genotypes of F. graminearum and F. culmorum isolates in Danish small grain cereals

International Journal of Food Microbiology

Volumn 157, Issue 3, 2012, Pages 384-392

  Nielsen, L K ^a   Jensen, J D ^b   Rodriguez A ^c   Jorgensen L N ^a   Justesen, A F ^a  

^a AARHUS UNIVERSITY   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c UNIVERSITY OF EXTREMADURA   (Spain)

Author keywords

Chemotype; Fusarium head blight; Genotypes; Mycotoxin; QPCR; Trichothecenes

Indexed keywords

15 ACETYL DEOXYNIVALENOL; 3 ACETYL DEOXYNIVALENOL; MYCOTOXIN; NIVALENOL; TRICHOTHECENE; UNCLASSIFIED DRUG;

ARTICLE; BARLEY; BIOMASS; CEREAL; CONTROLLED STUDY; FUNGAL GENE; FUNGUS ISOLATION; FUSARIUM; FUSARIUM CEREALIS; FUSARIUM CULMORUM; FUSARIUM GRAMINEARUM; FUSARIUM PSEUDOGRAMINEARUM; GENETIC POLYMORPHISM; GENOTYPE; GRAIN; NONHUMAN; OAT; PRINCIPAL COMPONENT ANALYSIS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RYE; TRITICALE;

CEREALS; FUNGAL PROTEINS; FUSARIUM; GENOTYPE; HORDEUM; MEMBRANE PROTEINS; POLYMORPHISM, GENETIC; REAL-TIME POLYMERASE CHAIN REACTION; SECALE CEREALE; TRICHOTHECENES; TRITICUM;

AVENA; FUSARIUM; FUSARIUM CEREALIS; FUSARIUM CULMORUM; FUSARIUM POAE; FUSARIUM PSEUDOGRAMINEARUM; GIBBERELLA ZEAE; HORDEUM; SECALE CEREALE; TRITICOSECALE; TRITICUM AESTIVUM;

EID: 84863879990     PISSN: 01681605     EISSN: 18793460     Source Type: Journal    
DOI: 10.1016/j.ijfoodmicro.2012.06.010     Document Type: Article

References (53)

1. Alexander N.J., McCormick S.P., Waalwijk C., van der Lee T., Proctor R.H. The genetic basis for 3-ADON and 15-ADON trichothecene chemotypes in Fusarium. Fungal Genetics and Biology 2011, 48:485-495.
2. Anon. Commission recommendation on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins in products intended for animal feeding. Official Journal of the European Union 2006, L229:7-9.
3. Anon. Commission Regulation (EC) no 1881/2006 setting maximum levels of certain contaminants in foodstuffs. Official Journal of the European Union 2006, 5-24.
4. Astolfi P., dos Santos J., Schneider L., Gomes L.B., Silva C.N., Tessmann D.J., Del Ponte E.M. Molecular survey of trichothecene genotypes of Fusarium graminearum species complex from barley in Southern Brazil. International Journal of Food Microbiology 2011, 148:197-201.
5. Bottalico A., Perrone G. Toxigenic Fusarium species and mycotoxins associated with head blight in small-grain cereals in Europe. European Journal of Plant Pathology 2002, 108:611-624.
6. Burlakoti R.R., Neate S.M., Adhikari T.B., Gyawali S., Salas B., Steffenson B.J., Schwarz P.B. Trichothecene profiling and population genetic analysis of Gibberella zeae from barley in North Dakota and Minnesota. Phytopathology 2011, 101:687-695.
7. Champeil A., Fourbet J.F., Dore T., Rossignol L. Influence of cropping system on Fusarium head blight and mycotoxin levels in winter wheat. Crop Protection 2004, 23:531-537.
8. Chandler E.A., Simpson D.R., Thomsett M.A., Nicholson P. Development of PCR assays to Tri7 and Tri13 trichothecene biosynthetic genes, and characterisation of chemotypes of Fusarium graminearum, Fusarium culmorum and Fusarium cerealis. Physiological and Molecular Plant Pathology 2003, 62:355-367.
9. Christensen B.T., Petersen J., Trentemøller U.M. The Askov long-term experiments on animal manure and mineral fertilizers: the Lermarken site 1894-2004. Plant Production 2006, vol. 121.
10. de Kuppler A.L., Steiner U., Sulyok M., Krska R., Oerke E.C. Genotyping and phenotyping of Fusarium graminearum isolates from Germany related to their mycotoxin biosynthesis. International Journal of Food Microbiology 2011, 151:78-86.
11. Desjardins A.E. Fusarium Mycotoxins: Chemistry, Genetics and Biology 1996, APS Press, St Paul, MN, USA.
12. Eriksen G.S., Pettersson H., Lundh T. Comparative cytotoxicity of deoxynivalenol, nivalenol, their acetylated derivatives and de-epoxy metabolites. Food and Chemical Toxicology 2004, 42:619-624.
13. Gale L.R., Ward T.J., Balmas V., Kistler H.C. Population subdivision of Fusarium graminearum sensu stricto in the upper Midwestern United States. Phytopathology 2007, 97:1434-1439.
14. Gale L.R., Harrison S.A., Ward T.J., O'Donnell K., Milus E.A., Gale S.W., Kistler H.C. Nivalenol-type populations of Fusarium graminearum and F. asiaticum are prevalent on wheat in southern Louisiana. Phytopathology 2011, 101:124-134.
15. Guo X.W., Fernando W.G.D., Seow-Brock H.Y. Population structure, chemotype diversity, and potential chemotype shifting of Fusarium graminearum in wheat fields of Manitoba. Plant Disease 2008, 92:756-762.
16. Hammer Ø., Harper D., Ryan P.D. PAST: paleontological statistics software package for education and data analysis. Palaeontologia Electronica 2001, 4:1-9.
17. Jennings P., Coates M.E., Turner J.A., Chandler E.A., Nicholson P. Determination of deoxynivalenol and nivalenol chemotypes of Fusarium culmorum isolates from England and Wales by PCR assay. Plant Pathology 2004, 53:182-190.
18. Jennings P., Coates M.E., Walsh K., Turner J.A., Nicholson P. Determination of deoxynivalenol- and nivalenol-producing chemotypes of Fusarium graminearum isolated from wheat crops in England and Wales. Plant Pathology 2004, 53:643-652.
19. Kammoun L.G., Gargouri S., Barreau C., Richard-Forget F., Hajlaoui M.R. Trichothecene chemotypes of Fusarium culmorum infecting wheat in Tunisia. International Journal of Food Microbiology 2010, 140:84-89.
20. Kulik T. Development of TaqMan assays for 3ADON, 15ADON and NIV Fusarium genotypes based on Tri12 gene. Cereal Research Communications 2011, 39:200-214.
21. Láday M., Juhász Á., Mulè G., Moretti A., Szécsi Á., Logrieco A. Mitochondrial DNA diversity and lineage determination of European isolates of Fusarium graminearum (Gibberella zeae). European Journal of Plant Pathology 2004, 110:545-550.
22. Langseth W., Bernhoft A., Rundberget T., Kosiak B., Gareis M. Mycotoxin production and cytotoxicity of Fusarium strains isolated from Norwegian cereals. Mycopathologia 1999, 144:103-113.
23. Leslie J.F., Visconti A., International C.A.B. Mycotoxins: Detection Methods, Management, Public Health, and Agricultural Trade 2008, CAB International, Trowbridge, United Kingdom.
24. Luongo D., De Luna R., Russo R., Severino L. Effects of four Fusarium toxins (fumonisin B(1), alpha-zearalenol, nivalenol and deoxynivalenol) on porcine whole-blood cellular proliferation. Toxicon 2008, 52:156-162.
25. Miedaner T., Schilling A.G., Geiger H.H. Molecular genetic diversity and variation for aggressiveness in populations of Fusarium graminearum and Fusarium culmorum sampled from wheat fields in different countries. Journal of Phytopathology 2001, 149:641-648.
26. Minervini F., Fornelli F., Flynn K.M. Toxicity and apoptosis induced by the mycotoxins nivalenol, deoxynivalenol and fumonisin B-1 in a human erythroleukemia cell line. Toxicology In Vitro 2004, 18:21-28.
27. Nicolaisen M., Suproniene S., Nielsen L.K., Lazzaro I., Spliid N.H., Justesen A.F. Real-time PCR for quantification of eleven individual Fusarium species in cereals. Journal of Microbiological Methods 2009, 76:234-240.
28. Nielsen L.K., Jensen J.D., Nielsen G.C., Jensen J.E., Spliid N.H., Thomsen I.K., Justesen A.F., Collinge D.B., Jorgensen L.N. Fusarium head blight of cereals in Denmark: species complex and related mycotoxins. Phytopathology 2011, 101:960-969.
29. O'Donnell K., Ward T.J., Geiser D.M., Corby Kistler H., Aoki T. Genealogical concordance between the mating type locus and seven other nuclear genes supports formal recognition of nine phylogenetically distinct species within the Fusarium graminearum clade. Fungal Genetics and Biology 2004, 41:600-623.
30. O'Donnell K., Kistler H.C., Tacke B.K., Casper H.H. Gene genealogies reveal global phylogeographic structure and reproductive isolation among lineages of Fusarium graminearum, the fungus causing wheat scab. Proceedings of the National Academy of Sciences of the United States of America 2000, 97:7905-7910.
31. Parry D.W., Jenkinson P., Mcleod L. Fusarium ear blight (scab) in small-grain cereals - a review. Plant Pathology 1995, 44:207-238.
32. Pasquali M., Giraud F., Brochot C., Cocco E., Hoffmann L., Bohn T. Genetic Fusarium chemotyping as a useful tool for predicting nivalenol contamination in winter wheat. International Journal of Food Microbiology 2010, 137:246-253.
33. Prodi A., Tonti S., Nipoti P., Pancaldi D., Pisi A. Identification of deoxynivalenol and nivalenol producing chemotypes of Fusarium graminearum isolates from durum wheat in a restricted area of Northern Italy. Journal of Plant Pathology 2009, 91:727-731.
34. Puri K.D., Zhong S. The 3ADON population of Fusarium graminearum found in North Dakota is more aggressive and produces a higher level of DON than the prevalent 15ADON population in spring wheat. Phytopathology 2010, 100:1007-1014.
35. Quarta A., Mita G., Haidukowski M., Santino A., Mule G., Visconti A. Assessment of trichothecene chemotypes of Fusarium culmorum occurring in Europe. Food Additives and Contaminants 2005, 22:309-315.
36. Quarta A., Mita G., Haidukowski M., Logrieco A., Mule G., Visconti A. Multiplex PCR assay for the identification of nivalenol, 3- and 15-acetyl-deoxynivalenol chemotypes in Fusarium. FEMS Microbiology Letters 2006, 259:7-13.
37. Reynoso M.M., Ramirez M.L., Torres A.M., Chulze S.N. Trichothecene genotypes and chemotypes in Fusarium graminearum strains isolated from wheat in Argentina. International Journal of Food Microbiology 2011, 145:444-448.
38. Sampietro D.A., Marín P., Iglesias J., Presello D.A., Vattuone M.A., Catalan C.A.N., Gonzalez Jaen M.T. A molecular based strategy for rapid diagnosis of toxigenic Fusarium species associated to cereal grains from Argentina. Fungal Biology 2010, 114:74-81.
39. Schmale D.G., Wood-Jones A.K., Cowger C., Bergstrom G.C., Arellano C. Trichothecene genotypes of Gibberella zeae from winter wheat fields in the Eastern USA. Plant Pathology 2011, 60:909-917.
40. Starkey D.E., Ward T.J., Aoki T., Gale L.R., Kistler H.C., Geiser D.M., Suga H., Toth B., Varga J., O'Donnell K. Global molecular surveillance reveals novel Fusarium head blight species and trichothecene toxin diversity. Fungal Genetics and Biology 2007, 44:1191-1204.
41. Stepien L., Popiel D., Koczyk G., Chelkowski J. Wheat-infecting Fusarium species in Poland-their chemotypes and frequencies revealed by PCR assay. Journal of Applied Genetics 2008, 49:433-441.
42. Suga H., Karugia G.W., Ward T., Gale L.R., Tomimura K., Nakajima T., Miyasaka A., Koizumi S., Kageyama K., Hyakumachi M. Molecular characterization of the Fusarium graminearum species complex in Japan. Phytopathology 2008, 98:159-166.
43. Szécsi Á., Bartók T., Varga M., Magyar D., Mesterházy Á. Determination of trichothecene chemotypes of Fusarium graminearum strains isolated in Hungary. Journal of Phytopathology 2005, 153:445-448.
44. Talas F., Parzies H.K., Miedaner T. Diversity in genetic structure and chemotype composition of Fusarium graminearum sensu stricto populations causing wheat head blight in individual fields in Germany. European Journal of Plant Pathology 2011, 131:39-48.
45. Tóth B., Mesterházy Á., Horváth Z., Bartók T., Varga M., Varga J. Genetic variability of Central European isolates of the Fusarium graminearum species complex. European Journal of Plant Pathology 2005, 113:35-45.
46. von der Ohe C., Gauthier V., Tamburic-Ilincic L., Brule-Babel A., Fernando W.G.D., Clear R., Ward T.J., Miedaner T. A comparison of aggressiveness and deoxynivalenol production between Canadian Fusarium graminearum isolates with 3-acetyl and 15-acetyldeoxynivalenol chemotypes in field-grown spring wheat. European Journal of Plant Pathology 2010, 127:407-417.
47. Waalwijk C., Kastelein P., de Vries I., Kerenyi Z., van der Lee T., Hesselink T., Kohl J., Kema G. Major changes in Fusarium spp. in wheat in the Netherlands. European Journal of Plant Pathology 2003, 109:743-754.
48. Wang J.H., Li H.P., Qu B., Zhang J.B., Huang T., Chen F.F., Liao Y.C. Development of a generic PCR detection of 3-acetyldeoxynivalenol-, 15-acetyldeoxynivalenol- and aivalenol-chemotypes of Fusarium graminearum clade. International Journal of Molecular Sciences 2008, 9:2495-2504.
49. Ward T.J., Bielawski J.P., Kistler H.C., Sullivan E., O'Donnell K. Ancestral polymorphism and adaptive evolution in the trichothecene mycotoxin gene cluster of phytopathogenic Fusarium. Proceedings of the National Academy of Sciences of the United States of America 2002, 99:9278-9283.
50. Ward T.J., Clear R.M., Rooney A.P., O'Donnell K., Gaba D., Patrick S., Starkey D.E., Gilbert J., Geiser D.M., Nowicki T.W. An adaptive evolutionary shift in Fusarium head blight pathogen populations is driving the rapid spread of more toxigenic Fusarium graminearum in North America. Fungal Genetics and Biology 2008, 45:473-484.
51. Yli-Mattila T. Ecology and evolution of toxigenic Fusarium species in cereals in Northern Europe and Asia. Journal of Plant Pathology 2010, 92:7-18.
52. Yli-Mattila T., Gagkaeva T. Molecular Chemotyping of Fusarium graminearum, F. culmorum, and F. cerealis Isolates from Finland and Russia Molecular Identification of Fungi 2010, 159-177. Springer, Berlin Heidelberg. Y. Gherbawy, K. Voigt (Eds.).
53. Yli-Mattila T., Gagkaeva T., Ward T.J., Aoki T., Kistler H.C., O'Donnell K. A novel Asian clade within the Fusarium graminearum species complex includes a newly discovered cereal head blight pathogen from the Russian Far East. Mycologia 2009, 101:841-852.