Single oocyte bisulfite mutagenesis

Journal of Visualized Experiments

Volumn , Issue 64, 2012, Pages 1-

  Denomme, Michelle M ^a,b   Zhang, Liyue ^b   Mann, Mellissa R W ^a,b  

^a WESTERN UNIVERSITY   (Canada)

^b Children's Health Research Institute   (United States)

Author keywords

Biochemistry; Bisulfite mutagenesis; Developmental biology; DNA methylation; Epigenetics; Genetics; Individual embryo; Individual oocyte; Issue 64; Mouse model; PCR

Indexed keywords

EID: 84863792499     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/4046     Document Type: Article

References (14)

1. Jaenisch, R. & Bird, A. Epigenetic regulation of gene expression: how the genome integrates intrinsic and environmental signals. Nat. Genet. 33 Suppl., 245-254 (2003).
2. Rodenhiser, D. & Mann, M. Epigenetics and human disease: translating basic biology into clinical applications. CMAJ. 174, 341-348 (2006).
3. Frommer, M., et al. A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands. Proc. Natl. Acad. Sci. U.S.A. 89, 1827-1831 (1992).
4. Clark, S.J., Harrison, J., Paul, C.L., & Frommer, M. High sensitivity mapping of methylated cytosines. Nucleic Acids Res. 22, 2990-2997 (1994).
5. Feil, R., Charlton, J., Bird, A.P., Walter, J., & Reik, W. Methylation analysis on individual chromosomes: improved protocol for bisulphite genomic sequencing. Nucleic Acids Res. 22, 695-696 (1994).
6. Raizis, A.M., Schmitt, F., & Jost, J.P. A bisulfite method of 5-methylcytosine mapping that minimizes template degradation. Anal. Biochem. 226, 161-166 (1995).
7. Patterson, K., Molloy, L., Qu, W., & Clark, S. DNA Methylation: Bisulphite Modification and Analysis. J. Vis. Exp. (56), e3170, DOI: 10.3791/3170 (2011).
8. Olek, A., Oswald, J., & Walter, J. A modified and improved method for bisulphite based cytosine methylation analysis. Nucleic Acids Res. 24, 5064-5066 (1996).
9. Mann, M.R., et al. Selective loss of imprinting in the placenta following preimplantation development in culture. Development. 131, 3727-3735, (2004).
10. Market-Velker, B.A., Zhang, L., Magri, L.S., Bonvissuto, A.C., & Mann, M.R. Dual effects of superovulation: loss of maternal and paternal imprinted methylation in a dose-dependent manner. Hum. Mol. Genet. 19, 36-51 (2010).
11. Meng, L.H., Xiao, S.Q., Huang, X.F., Zhou, Y., & Xu, B.S. [A study on bisulfite sequencing method for methylation status of imprinted genes in single human oocytes]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 25, 289-292 (2008).
12. Denomme, M.M., Zhang, L., & Mann, M.R. Embryonic imprinting perturbations do not originate from superovulation-induced defects in DNA methylation acquisition. Fertil. Steril. 96, 734-738 (2011).
13. Tahiliani, M., et al. Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1. Science. 324, 930-935 (2009).
14. El Hajj, N., et al. Limiting dilution bisulfite (pyro)sequencing reveals parent-specific methylation patterns in single early mouse embryos and bovine oocytes. Epigenetics. 6, 1176-1188 (2011).