Constant pressure-controlled extrusion method for the preparation of nano-sized lipid vesicles

Journal of Visualized Experiments

Volumn , Issue 64, 2012, Pages

  Morton, Leslie A ^a   Saludes, Jonel P ^a   Yin, Hang ^a  

^a UNIVERSITY OF COLORADO   (United States)

Author keywords

Bioengineering; Biomedical engineering; Dynamic light scattering (DLS); Issue 64; Liposomes; Nano sized vesicles; Nanoparticle tracking analysis (NTA); Particle extrusion

Indexed keywords

EID: 84863786740     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/4151     Document Type: Article

References (13)

1. Connor, J., Bucana, C., Fidler, I.J., & Schroit, A.J. Differentiation-dependent expression of phosphatidylserine in mammalian plasma membranes: quantitative assessment of outer-leaflet lipid by prothrombinase complex formation. Proc. Natl. Acad. Sci. U.S.A. 86 (9), 3184-3188 (1989).
2. Smith, S.A. & Morrissey, J.H. Rapid and efficient incorporation of tissue factor into liposomes. J. Thromb. Haemost. 2 (7), 1155-1162 (2004).
3. Hui, E., Johnson, C.P., Yao, J., Dunning, F.M., & Chapman, E.R. Synaptotagmin-mediated bending of the target membrane is a critical step in Ca(2+)-regulated fusion. Cell. 138 (4), 709-721 (2009).
4. Loughrey, H.C., Choi, L.S., Cullis, P.R., & Bally, M.B. Optimized procedures for the coupling of proteins to liposomes. J. Immunol. Methods. 132 (1), 25-35 (1990).
5. Mui, B., Chow, L., & Hope, M.J., Extrusion technique to generate liposomes of defined size. Methods Enzymol. 367, 3-14 (2003).
6. Gruner, S.M., Cullis, P.R., Hope, M.J., & Tilcock, C.P. Lipid polymorphism: the molecular basis of nonbilayer phases. Annu. Rev. Biophys. Biophys. Chem. 14, 211-238 (1985).
7. Guven, A., Ortiz, M., Constanti, M., & O'Sullivan, C.K. Rapid and efficient method for the size separation of homogeneous fluorescein-encapsulating liposomes. J. Liposome. Res. 19 (2), 148-154 (2009).
8. Zimmerberg, J. & Kozlov, M. How proteins produce cellular membrane curvature. Nature Reviews Molecular Cell Biology. 7, 9-19 (2006).
9. Chonn, A. & Cullis, P.R. Recent advances in liposomal drug-delivery systems. Curr. Opin. Biotechnol. 6 (6), 698-708 (1995).
10. Mayer, L.D., Hope, M.J., & Cullis, P.R. Vesicles of variable sizes produced by a rapid extrusion procedure. Biochim. Biophys. Acta. 858 (1), 161-168 (1986).
11. Matsuoka, K. & Schekman, R. The use of liposomes to study COPII- and COPI-coated vesicle formation and membrane protein sorting. Methods. 20 (4), 417-428 (2001).
12. Dragovic, R.A., Gardiner, C., Brooks, A.S., Tannetta, D.S., Ferguson, D., Hole, P., Carr, B., Redman, C., Harris, A.L., Dobson, P.J., Harrison, P., & Sargent, I.L. Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis. Nanomedicine (2011).
13. Hunter, D.G. & Frisken, B.J. Effect of extrusion pressure and lipid properties on the size and polydispersity of lipid vesicles. Biophys J. 74 (6), 2996-3002 (1998).