Rapid detection of foot-and-mouth disease virus using a field-portable nucleic acid extraction and real-time PCR amplification platform

Veterinary Journal

Volumn 193, Issue 1, 2012, Pages 67-72

  Madi, Mikidache ^a   Hamilton, Andrea ^b   Squirrell, David ^b   Mioulet, Valerie ^a   Evans, Phillip ^b   Lee, Martin ^b   King, Donald P ^a  

^a INSTITUTE FOR ANIMAL HEALTH   (United Kingdom)

^b Tetricus Science Park   (United Kingdom)

Author keywords

Field diagnostics; Foot and mouth disease virus; Real time reverse transcriptase PCR

Indexed keywords

ARTICLE; AUTOMATION; FOOT AND MOUTH DISEASE VIRUS; GENE AMPLIFICATION; INTERMETHOD COMPARISON; LIMIT OF DETECTION; NONHUMAN; PORTABLE EQUIPMENT; PROCESS OPTIMIZATION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; VIRUS DETECTION;

ANIMALS; AUTOMATION, LABORATORY; CATTLE; CATTLE DISEASES; FOOT-AND-MOUTH DISEASE; FOOT-AND-MOUTH DISEASE VIRUS; REAGENT KITS, DIAGNOSTIC; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL;

FOOT-AND-MOUTH DISEASE VIRUS;

EID: 84863779812     PISSN: 10900233     EISSN: 15322971     Source Type: Journal    
DOI: 10.1016/j.tvjl.2011.10.017     Document Type: Article

References (19)

1. Anderson, I., 2002. Foot and Mouth Disease 2001: Lessons to be Learned Inquiry Report. The Stationery Office, HC 888 Session 2001-2002.
2. Anonymous, 2002a. The 2001 Outbreak of Foot and Mouth Disease. Report by the Controller and Auditor General. National Audit Office HC 939 Session 2001-2002.
3. Anonymous Royal Society Inquiry into Infectious Diseases in Livestock 2002, Royal Society, London.
4. Callahan J.D., Brown F., Osorio F.A., Sur J.H., Kramer E., Long G.W., Lubroth J., Ellis S.J., Shoulars K.S., Gaffney K.L., Rock D.L., Nelson W.M. Use of a portable real-time reverse transcriptase-polymerase chain reaction assay for rapid detection of foot-and-mouth disease virus. Journal of the American Veterinary Medical Association 2002, 220:1636-1642.
5. Ferris N.P., Dawson M. Routine application of enzyme-linked immunosorbent assay in comparison with complement fixation for the diagnosis of foot-and-mouth and swine vesicular diseases. Veterinary Microbiology 1988, 16:201-209.
6. Ferris N.P., King D.P., Reid S.M., Shaw A.E., Hutchings G.H. Analysis of original laboratory results and retrospective analysis by real-time RT-PCR of virological samples collected from confirmed cases suggest over-reporting of foot-and-mouth disease in the UK in 2001. Veterinary Record 2006, 159:373-378.
7. Ferris N.P., Hutchings G.H., Reid S.M., King D.P., Ebert K., Paton D.J., Brocchi E., Nordengrahn A., Merza M. Development and laboratory validation of a lateral flow device for the detection of foot-and-mouth disease virus in clinical samples. Journal of Virological Methods 2009, 155:10-17.
8. King D.P., Reid S.M., Hutchings G.H., Grierson S.S., Wilkinson P.J., Dixon L.K., Bastos A.D.S., Drew T.W. Development of a TaqMan PCR with internal amplification control for the detection of African swine fever virus. Journal of Virological Methods 2003, 107:53-61.
9. King D.P., Dukes J.P., Reid S.M., Ebert K., Shaw A.E., Mills C.E., Boswell L., Ferris N.P. Prospects for rapid diagnosis of foot-and-mouth disease in the field using RT-PCR. Veterinary Record 2008, 162:315-316.
10. Logan J., Edwards K., Saunders N. Real-time PCR: Current Technology and Applications 2009, Caister Academic Press, Norfolk, UK, 284 pp.
11. Reid S.M., Ferris N.P., Hutchings G.H., Zhang Z., Belsham G.J., Alexandersen S. Detection of all seven serotypes of foot-and-mouth disease virus by real-time, fluorogenic reverse-transcription polymerase chain reaction assay. Journal of Virological Methods 2002, 105:67-80.
12. Reid S.M., Grierson S.S., Ferris N.P., Hutchings G.H., Alexandersen S. Evaluation of automated RT-PCR to accelerate the laboratory diagnosis of foot-and-mouth disease virus. Journal of Virological Methods 2003, 107:129-139.
13. Reid S.M., Ebert K., Bachanek-Bankowska K., Batten C., Sanders A., Wright C., Shaw A.E., Ryan E.D., Hutchings G.H., Ferris N.P., Paton D.J., King D.P. Performance of real-time RT-PCR for the detection of foot-and-mouth disease virus during field outbreaks in the United Kingdom in 2007. Journal of Veterinary Diagnostic Investigation 2009, 21:321-330.
14. Ryan E., Gloster J., Reid S.M., Li Y., Ferris N.P., Waters R., Juleff N., Charleston B., Bankowski B., Gubbins S., Wilesmith J., King D.P., Paton D.J. Clinical and laboratory investigations of the foot-and-mouth disease epidemic in the United Kingdom in 2007. Veterinary Record 2008, 163:139-147.
15. Sammin D., Ryan E., Ferris N.P., King D.P., Zientara S., Haas B., Yadin H., Alexandersen S., Sumption K., Paton D.J. Options for decentralised testing of suspected secondary outbreaks of foot-and-mouth disease. Transboundary and Emerging Diseases 2010, 57:237-243.
16. Scudamore J.M., Harris D.M. Control of foot and mouth disease: Lessons from the experience of the outbreak in Great Britain in 2001. Revue Scientifique et Technique. Office International des Epizootes 2002, 21:699-710.
17. Shaw A.E., Reid S.M., King D.P., Hutchings G.H., Ferris N.P. Enhanced laboratory diagnosis of foot and mouth disease by real-time polymerase chain reaction. Revue Scientifique et Technique. Office International des Epizootes 2004, 23:1003-1009.
18. Shaw A.E., Reid S.M., Ebert K., Hutchings G.H., Ferris N.P., King D.P. Implementation of a one-step real-time RT-PCR protocol for diagnosis of foot-and-mouth disease. Journal of Virological Methods 2007, 143:81-85.
19. Wakeley P.R., Errington J., Squirrell D. Use of a field-enabled nucleic acid extraction and PCR instrument to detect BVDV. Veterinary Record 2010, 166:238-239.