Oct4GiP reporter assay to study genes that regulate mouse embryonic stem cell maintenance and self-renewal

Journal of Visualized Experiments

Volumn , Issue 63, 2012, Pages

  Zheng, Xiaofeng ^a   Hu, Guang ^a  

^a NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

Author keywords

Differentiation; Embryonic stem cell; ESC; Genetics; GFP; Issue 63; Molecular biology; Oct4; Reporter assay; RNAi; Self renewal; Stem cell biology

Indexed keywords

EID: 84863734814     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3987     Document Type: Review

References (19)

1. Keller G. Embryonic stem cell differentiation: emergence of a new era in biology and medicine. Genes Dev. 19 (10), 1129-1155. doi:10.1101/gad.1303605 (2005).
2. Murry, C.E. & Keller, G. Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development. Cell. 132, 661-680. doi:10.1016/j.cell.2008.02.008 (2008).
3. Hu, G., et al. A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal. Genes Dev. 23, 837-848. doi: 10.1101/gad.1769609 [pii] 23/7/837 (2009).
4. Ying, Q.L., Nichols, J., Evans, E.P., & Smith, A.G. Changing potency by spontaneous fusion. Nature. 416, 545-548. doi:10.1038/nature729 [pii] nature729 (2002).
5. Ying, Q.L. & Smith, A.G. Defined conditions for neural commitment and differentiation. Methods Enzymol. 365, 327-341 doi:10.1016/S0076-6879(03)65023-8 (2003).
6. Nichols, J., et al. Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell. 95, 379-391, [pii] S0092-8674(00)81769-9 (1998).
7. Niwa, H., Miyazaki, J., & Smith, A.G. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat. Genet. 24, 372-376, doi:10.1038/74199 (2000).
8. Ding, L., et al. A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity. Cell Stem Cell. 4, 403-415, doi:10.1016/j.stem.2009.03.009 (2009).
9. Chambers, I., et al. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell. 113, 643-655, [pii] S0092867403003921 (2003).
10. Masui, S., et al. Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nat. Cell Biol. 9, 625-635, doi:10.1038/ncb1589 [pii] ncb1589 (2007).
11. Mitsui, K., et al. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell. 113, 631-642, [pii] S0092867403003933 (2003).
12. Fazzio, T.G., Huff, J.T., & Panning, B. An RNAi screen of chromatin proteins identifies Tip60-p400 as a regulator of embryonic stem cell identity. Cell. 134, 162-174, doi:10.1016/j.cell.2008.05.031 (2008).
13. Pease, S., Braghetta, P., Gearing, D., Grail, D., & Williams, R.L. Isolation of embryonic stem (ES) cells in media supplemented with recombinant leukemia inhibitory factor (LIF). Dev. Biol. 141, 344-352 doi:10.1016/0012-1606(90)90390-5 (1990).
14. Young, R.A. Control of the embryonic stem cell state. Cell. 144, 940-954, doi:10.1016/j.cell.2011.01.032 (2011).
15. Chambers, I., et al. Nanog safeguards pluripotency and mediates germline development. Nature. 450, 1230-1234, [pii] nature06403 doi:10.1038/nature06403 (2007).
16. Maherali, N., et al. Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution. Cell Stem Cell. 1, 55-70, [pii] S1934-5909(07)00020-3 doi:10.1016/j.stem.2007.05.014 (2007).
17. Rodda, D.J., et al. Transcriptional regulation of nanog by OCT4 and SOX2. J. Biol. Chem. 280, 24731-24737, [pii] M502573200 doi: 10.1074/jbc.M502573200 (2005).
18. Schaniel, C., et al. Delivery of short hairpin RNAs--triggers of gene silencing--into mouse embryonic stem cells. Nat. Methods. 3, 397-400, [pii] nmeth0506-397 doi:10.1038/nmeth0506-397 (2006).
19. Toyooka, Y., Shimosato, D., Murakami, K., Takahashi, K., & Niwa, H. Identification and characterization of subpopulations in undifferentiated ES cell culture. Development. 135, 909-918, doi:135/5/909 [pii] 10.1242/dev.017400 (2008).