A low-cost affinity purification system using β-1,3-glucan recognition protein and curdlan beads

Protein Engineering, Design and Selection

Volumn 25, Issue 8, 2012, Pages 405-413

  Horiuchi, Masataka ^a   Takahasi, Kiyohiro ^b   Kobashigawa, Yoshihiro ^a   Ochiai, Masanori ^a   Inagaki, Fuyuhiko ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

1,3 glucan recognition protein; affinity tag; curdlan; glutathione S transferase; recombinant protein

Indexed keywords

AFFINITY PURIFICATION; AFFINITY TAG; ALKALINE SOLUTIONS; CREATIVE COMMONS; CURDLAN; EXPRESSION LEVELS; GLUTATHIONE-S-TRANSFERASE; GLUTATHIONES; LOW COSTS; OPEN ACCESS; OXFORD UNIVERSITY; SOLUBLE FRACTION; TAGGED PROTEINS;

ALKALINITY; CENTRIFUGATION; PURIFICATION; RECOMBINANT PROTEINS; STRUCTURAL ANALYSIS;

FOOD ADDITIVES;

BETA 1,3 GLUCAN; BETA 1,3 GLUCAN RECOGNITION PROTEIN; BINDING PROTEIN; CURDLAN; CURDLAN BEAD; GLUTATHIONE; GLUTATHIONE TRANSFERASE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CENTRIFUGATION; COMPLEX FORMATION; MOLECULAR INTERACTION; MOLECULAR RECOGNITION; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN STRUCTURE; SILKWORM; SOLUBILIZATION; ULTRASOUND;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BETA-GLUCANS; BOMBYX; CARRIER PROTEINS; CHROMATOGRAPHY, AFFINITY; DEAD-BOX RNA HELICASES; ESCHERICHIA COLI; GLUTATHIONE TRANSFERASE; HUMANS; INSECT PROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN STABILITY; RECOMBINANT FUSION PROTEINS;

BOMBYX MORI;

EID: 84863702142     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzs028     Document Type: Article

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