Acoustic deposition with NIMS as a high-throughput enzyme activity assay

Analytical and Bioanalytical Chemistry

Volumn 403, Issue 3, 2012, Pages 707-711

  Greving, Matthew ^a   Cheng, Xiaoliang ^b   Reindl, Wolfgang ^b   Bowen, Benjamin ^b   Deng, Kai ^b,c   Louie, Katherine ^b   Nyman, Michael ^a   Cohen, Joseph ^a   Singh, Anup ^b,c   Simmons, Blake ^b,c   Adams, Paul ^b   Siuzdak, Gary ^d   Northen, Trent ^b  

^a Nextval   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^c SANDIA NATIONAL LABORATORIES   (United States)

^d SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Enzyme assay; Glycoside hydrolase; Nanostructure initiator mass spectrometry; NIMS; Nimzyme

Indexed keywords

ENVIRONMENTAL SAMPLE; ENZYMATIC ACTIVITIES; ENZYMATIC REACTION; ENZYME ASSAYS; GLYCOSIDE HYDROLASES; HIGH-THROUGHPUT; LIQUID-LIQUIDS; MANUAL ANALYSIS; NIMS; NIMZYME; RAPID COMMUNICATION; REACTION PATHWAYS; SAMPLE DEPOSITION;

ENZYME ACTIVITY; LIQUIDS; MASS SPECTROMETRY; NANOSTRUCTURES; VISUALIZATION;

SCREENING;

GLYCOSIDASE; NANOMATERIAL; XYLAN 1,4 BETA XYLOSIDASE;

ACOUSTICS; ASPERGILLUS NIGER; BACILLUS; CHEMISTRY; CONFERENCE PAPER; ECONOMICS; ENZYME ASSAY; ENZYMOLOGY; EQUIPMENT DESIGN; HIGH THROUGHPUT SCREENING; INSTRUMENTATION; MASS SPECTROMETRY; METABOLISM; TIME;

ACOUSTICS; ASPERGILLUS NIGER; BACILLUS; ENZYME ASSAYS; EQUIPMENT DESIGN; GLYCOSIDE HYDROLASES; HIGH-THROUGHPUT SCREENING ASSAYS; NANOSTRUCTURES; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TIME FACTORS; XYLOSIDASES;

EID: 84863412250     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-012-5908-8     Document Type: Conference Paper

References (10)

1. Blanch H, Adams P, Andrews-Cramer K, Frommer W, Simmons B, Keasling JD (2008) ACS Chem Biol 3:17-20
2. Stehen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Del Cardayre SB, Keasling JD (2010) Nature 463:559-562
3. Kim T, Chokhawala H, Hess M, Dana C, Baer Z, Sczyba A, Rubin E, Blanch H, Clark D (2011) Angew Chem 50:11215-11218
4. Sharrock K (1988) J Biochem Biophys Methods 17:81-105
5. Reindl W, Deng K, Gladden J, Cheng G, Wong A, Singer S, Singh S, Lee J, Yao C, Hazen T, Singh A, Simmons B, Adams P, Northen T (2011) Energy Environ Sci 4:2884-2893
6. Northen T, Lee J, Hoang L, Raymond J, Hwang D, Yannone S, Wong C, Siuzdak G (2008) Proc Natl Acad Sci USA 105:3678-3683
7. Northen T, Yanes O, Northen M, Marrinucci D, Uritboonthai D, Apon J, Golledge S, Nordstrom A, Siuzdak G (2007) Nature 449:1033-1036
8. Aerni H, Cornett D, Caprioli R (2006) Anal Chem 78:827-834
9. Woo K, Northen T, Yanes O, Siuzdak G (2008) Nature Prot 3:1341-1349
10. Deng K, George K, Reindl W, Keasling J, Adams P, Lee T, Singh A, Northen T (2011) Rapid Commun Mass Spectrom (in press)