Expression of recombinant mature human tyrosinase from escherichia coli and exhibition of its activity without phosphorylation or glycosylation

Journal of Agricultural and Food Chemistry

Volumn 60, Issue 11, 2012, Pages 2838-2843

  Chen, Gen Hung ^a   Chen, Wei Ming ^b   Huang, Ya Chi ^a   Jiang, Shann Tzong ^a,b  

^a PROVIDENCE UNIVERSITY   (Taiwan)

^b NATIONAL TAIWAN OCEAN UNIVERSITY   (Taiwan)

Author keywords

E. coli BL21(DE3); N terminal methionine excision; recombinant mature human tyrosinase; renaturation

Indexed keywords

CDNA ENCODING; E. COLI; INCLUSION BODIES; LACTAMASES; N-TERMINALS; OPTIMUM ACTIVITY; RECOMBINANT MATURE HUMAN TYROSINASE; RENATURATION; SEPHADEX;

AMINO ACIDS; CENTRIFUGATION; CLONING; GEL PERMEATION CHROMATOGRAPHY; ION EXCHANGE; PHOSPHORYLATION; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

MONOPHENOL MONOOXYGENASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; GLYCOSYLATION; HUMAN; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHOSPHORYLATION; TUMOR CELL LINE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CELL LINE, TUMOR; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GLYCOSYLATION; HUMANS; MOLECULAR SEQUENCE DATA; MONOPHENOL MONOOXYGENASE; PHOSPHORYLATION; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 84863341376     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf205021g     Document Type: Article

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