ROP11 GTPase Negatively Regulates ABA Signaling by Protecting ABI1 Phosphatase Activity from Inhibition by the ABA Receptor RCAR1/PYL9 in Arabidopsis

Journal of Integrative Plant Biology

Volumn 54, Issue 3, 2012, Pages 180-188

  Li, Zixing ^a   Li, Zheng ^a   Gao, Xiang ^a   Chinnusamy, Viswanathan ^b   Bressan, Ray ^c   Wang, Zhi Xin ^a   Zhu, Jian Kang ^c   Wu, Jia Wei ^a   Liu, Dong ^a  

^a TSINGHUA UNIVERSITY   (China)

^b INDIAN AGRICULTURAL RESEARCH INSTITUTE   (India)

^c PURDUE UNIVERSITY   (United States)

Author keywords

ABA signaling; ABI1; Arabidopsis; RCAR1 PYL9A; ROP11 GTPase

Indexed keywords

ABI1 PROTEIN, ARABIDOPSIS; ABSCISIC ACID; ARABIDOPSIS PROTEIN; CARRIER PROTEIN; PHOSPHOPROTEIN PHOSPHATASE; PROTEIN SERINE THREONINE KINASE; RCAR1 PROTEIN, ARABIDOPSIS; RHO GUANINE NUCLEOTIDE BINDING PROTEIN; ROP11 PROTEIN, ARABIDOPSIS; SNRK2 PROTEIN, ARABIDOPSIS;

ANGIOSPERM; BIOCHEMISTRY; FLUORESCENCE; GERMINATION; HORMONE; SEED;

ARABIDOPSIS; ARTICLE; CELL FRACTIONATION; DRUG ANTAGONISM; DRUG EFFECT; ENZYMOLOGY; IMMUNOPRECIPITATION; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; PROTEIN TRANSPORT; PROTOPLAST; SIGNAL TRANSDUCTION;

ABSCISIC ACID; ARABIDOPSIS; ARABIDOPSIS PROTEINS; CARRIER PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; IMMUNOPRECIPITATION; PHOSPHOPROTEIN PHOSPHATASES; PROTEIN BINDING; PROTEIN TRANSPORT; PROTEIN-SERINE-THREONINE KINASES; PROTOPLASTS; RHO GTP-BINDING PROTEINS; SIGNAL TRANSDUCTION; SUBCELLULAR FRACTIONS;

ARABIDOPSIS; ARABIDOPSIS THALIANA; NICOTIANA BENTHAMIANA;

EID: 84863337771     PISSN: 16729072     EISSN: 17447909     Source Type: Journal    
DOI: 10.1111/j.1744-7909.2012.01101.x     Document Type: Article

References (29)

1. Batoko H, Zheng HQ, Hawes C, Moore I (2000) A rab1 GTPase is required for transport between the endoplasmic reticulum and golgi apparatus and for normal golgi movement in plants. Plant Cell 12, 2201-2218.
2. Chen H, Zou Y, Shang Y, Lin H, Wang Y, Cai R, Tang X, Zhou JM (2008) Firefly luciferase complementation imaging assay for protein-protein interactions in plants. Plant Physiol. 146, 368-376.
3. Cutler SR, Rodriguez PL, Finkelstein RR, Abrams SR (2010) Abscisic acid: Emergence of a core signaling network. Annu. Rev. Plant Biol. 61, 651-679.
4. Finkelstein RR, Gampala SS, Rock CD (2002) Abscisic acid signaling in seeds and seedlings. Plant Cell 14 Suppl, S15-45.
5. Fujii H, Chinnusamy V, Rodrigues A, Rubio S, Antoni R, Park SY, Cutler SR, Sheen J, Rodriguez PL, Zhu JK (2009) In vitro reconstitution of an abscisic acid signalling pathway. Nature 462, 660-664.
6. Furihata T, Maruyama K, Fujita Y, Umezawa T, Yoshida R, Shinozaki K, Yamaguchi-Shinozaki K (2006) Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1. Proc. Natl. Acad. Sci. USA 103, 1988-1993.
7. Guo J, Yang X, Weston DJ, Chen JG (2011) Abscisic acid receptors: Past, present and future. J. Integr. Plant Biol. 53, 469-479.
8. Gosti F, Beaudoin N, Serizet C, Webb AA, Vartanian N, Giraudat J (1999) ABI1 protein phosphatase 2C is a negative regulator of abscisic acid signaling. Plant Cell 11, 1897-1910.
9. Gu Y, Li S, Lord EM, Yang Z (2006) Members of a novel class of Arabidopsis Rho guanine nucleotide exchange factors control Rho GTPase-dependent polar growth. Plant Cell 18, 366-381.
10. Hubbard KE, Nishimura N, Hitomi K, Getzoff ED, Schroeder JI (2010) Early abscisic acid signal transduction mechanisms: Newly discovered components and newly emerging questions. Genes Dev. 24, 1695-1708.
11. Johnson RR, Wagner RL, Verhey SD, Walker-Simmons MK (2002) The abscisic acid-responsive kinase PKABA1 interacts with a seed-specific abscisic acid response element-binding factor, TaABF, and phosphorylates TaABF peptide sequences. Plant Physiol. 130, 837-846.
12. Leonhardt N, Kwak JM, Robert N, Waner D, Leonhardt G, Schroeder JI (2004) Microarray expression analyses of Arabidopsis guard cells and isolation of a recessive abscisic acid hypersensitive protein phosphatase 2C mutant. Plant Cell 16, 596-615.
13. Li Z, Kang J, Sui N, Liu D (2012) ROP11 GTPase is a negative regulator of multiple ABA responses in Arabidopsis. J. Integr. Plant Biol. doi:
14. Ma Y, Szostkiewicz I, Korte A, Moes D, Yang Y, Christmann A, Grill E (2009) Regulators of PP2C phosphatase activity function as abscisic acid sensors. Science 324, 1064-1068
15. Merlot S, Gosti F, Guerrier D, Vavasseur A, Giraudat J (2001) The ABI1 and ABI2 protein phosphatases 2C act in a negative feedback regulatory loop of the abscisic acid signalling pathway. Plant J. 25, 295-303.
16. Moes D, Himmelbach A, Korte A, Haberer G, Grill E (2008) Nuclear localization of the mutant protein phosphatase abi1 is required for insensitivity towards ABA responses in Arabidopsis. Plant J. 54, 806-819.
17. Nibau C, Wu HM, Cheung AY (2006) RAC/ROP GTPases: 'Hubs' for signal integration and diversification in plants. Trends Plant Sci. 11, 309-315.
18. Nishimura N, Sarkeshik A, Nito K, Park SY, Wang A, Carvalho PC, Lee S, Caddell DF, Cutler SR, Chory J, Yates JR, Schroeder JI (2010) PYR/PYL/RCAR family members are major in-vivo ABI1 protein phosphatase 2C-interacting proteins in Arabidopsis. Plant J. 61, 290-299.
19. Nishimura N, Yoshida T, Kitahata N, Asami T, Shinozaki K, Hirayama T (2007) ABA-Hypersensitive Germination1 encodes a protein phosphatase 2C, an essential component of abscisic acid signaling in Arabidopsis seed. Plant J. 50, 935-949.
20. Park SY, Fung P, Nishimura N, Jensen DR, Fujii H, Zhao Y, Lumba S, Santiago J, Rodrigues A, Chow TF, Alfred SE, Bonetta D, Finkelstein R, Provart NJ, Desveaux D, Rodriguez PL, McCourt P, Zhu JK, Schroeder JI, Volkman BF, Cutler SR (2009) Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Science 324, 1068-1071.
21. Penfield S, Josse EM, Kannangara R, Gilday AD, Halliday KJ, Graham IA (2005) Cold and light control seed germination through the bHLH transcription factor SPATULA. Curr. Biol. 15, 1998-2006.
22. Rubio S, Rodrigues A, Saez A, Dizon MB, Galle A, Kim TH, Santiago J, Flexas J, Schroeder JI, Rodriguez PL (2009) Triple loss of function of protein phosphatases type 2C leads to partial constitutive response to endogenous abscisic acid. Plant Physiol. 150, 1345-1355.
23. Saez A, Apostolova N, Gonzalez-Guzman M, Gonzalez-Garcia MP, Nicolas C, Lorenzo O, Rodriguez PL (2004) Gain-of-function and loss-of-function phenotypes of the protein phosphatase 2C HAB1 reveal its role as a negative regulator of abscisic acid signalling. Plant J. 37, 354-369.
24. Santiago J, Rodrigues A, Saez A, Rubio S, Antoni R, Dupeux F, Park SY, Marquez JA, Cutler SR, Rodriguez PL (2009) Modulation of drought resistance by the abscisic acid receptor PYL5 through inhibition of clade A PP2Cs. Plant J. 60, 575-588.
25. Umezawa T, Sugiyama N, Mizoguchi M, Hayashi S, Myouga F, Yamaguchi-Shinozaki K, Ishihama Y, Hirayama T, Shinozaki K (2009) Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis. Proc. Natl. Acad. Sci. USA 106, 17588-17593.
26. Vlad F, Rubio S, Rodrigues A, Sirichandra C, Belin C, Robert N, Leung J, Rodriguez PL, Lauriere C, Merlot S (2009) Protein phosphatases 2C regulate the activation of the Snf1-related kinase OST1 by abscisic acid in Arabidopsis. Plant Cell 21, 3170-3184.
27. Yoshida R, Umezawa T, Mizoguchi T, Takahashi S, Takahashi F, Shinozaki K (2006) The regulatory domain of SRK2E/OST1/SnRK2.6 interacts with ABI1 and integrates abscisic acid (ABA) and osmotic stress signals controlling stomatal closure in Arabidopsis. J. Biol. Chem. 281, 5310-5318.
28. Yoshida T, Nishimura N, Kitahata N, Kuromori T, Ito T, Asami T, Shinozaki K, Hirayama T (2006) ABA-hypersensitive germination3 encodes a protein phosphatase 2C (AtPP2CA) that strongly regulates abscisic acid signaling during germination among Arabidopsis protein phosphatase 2Cs. Plant Physiol. 140, 115-126.
29. Zheng ZL, Yang Z (2000) The Rop GTPase: An emerging signaling switch in plants. Plant Mol. Biol. 44, 1-9.