Use of high-gradient magnetic fishing for reducing proteolysis during fermentation

Biotechnology Journal

Volumn 7, Issue 7, 2012, Pages 909-918

  Maury, Trine L ^a   Ottow, Kim E ^a   Brask, Jesper ^b   Villadsen, John ^a   Hobley, Timothy J ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b NOVOZYMES A S   (Denmark)

Author keywords

Bacillus; Biochemical engineering; Enzyme; High gradient magnetic separation; Subtilisin

Indexed keywords

BACILLUS LICHENIFORMIS; BINDING KINETICS; BOVINE SERUM ALBUMINS; CONVENTIONAL METHODS; CULTIVATION PROCESS; FERMENTATION BROTHS; HETEROLOGOUS PROTEINS; HIGH GRADIENT MAGNETIC SEPARATION; LANGMUIR ISOTHERM; MAGNETIC ADSORBENTS; MAGNETIC AFFINITY; MODEL PROTEINS; PROTEOLYTIC DEGRADATION; SHAKE-FLASK CULTURES; SPECIFIC BINDING; SUBTILISIN; TRACE AMOUNTS;

BACILLI; BACTERIOLOGY; BIOCHEMICAL ENGINEERING; DEGRADATION; ENZYMES; FERMENTERS; FISHERIES; MAGNETIC SEPARATION; PROTEINS;

FERMENTATION;

ADSORBENT; BOVINE SERUM ALBUMIN; PROTEINASE;

ARTICLE; BACILLUS LICHENIFORMIS; BACTERIAL GROWTH; BIOREACTOR; ENZYME ACTIVITY; FERMENTATION; HIGH GRADIENT MAGNETIC SEPARATION; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION;

ADSORPTION; ANIMALS; BACILLUS; BIOMASS; BIOTECHNOLOGY; CATTLE; FERMENTATION; KINETICS; MAGNETS; PEPTIDE HYDROLASES; PROTEIN BINDING; PROTEOLYSIS; SERUM ALBUMIN, BOVINE;

BACILLUS LICHENIFORMIS; BOVINAE;

EID: 84863301207     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201100376     Document Type: Article

References (27)

1. Bock, J., What to do when the platform fails: The science-based development of an HSA/GLP-1 fusion, in Recovery of biological products, XII. The Wigwam Resort, Litchfield Park 2006.
2. Wang, L., Ridgway, D., Gu, T., Moo-Young, M., Bioprocessing strategies to improve heterologous protein production in filamentous fungal fermentations. Biotechnol. Adv. 2005, 23, 115-129.
3. Zhao, H. L., Xue, C., Wang, Y., Yao, X. Q. et al., Increasing the cell viability and heterologous protein expression of Pichia pastoris mutant deficient in PMR1 gene by culture condition optimization. Appl. Microbiol. Biotechnol. 2008, 81, 235-241.
4. Kichakova, N. A., Pavlova, I. N., Zakharova, I. Y., Purification and identification of amylolytic enzymes from Bacillus Licheniformis. Appl. Biochem. Microbiol. 1998, 34, 458-461.
5. Idiris, A., Tohda, H., Bi, K. W., Isoai, A. et al., Enhanced productivity of protease-sensitive heterologous proteins by disruption of multiple protease genes in the fission yeast Schizosaccharomyces pombe. Appl. Microbiol. Biotechnol. 2006, 73, 404-420.
6. Kimura, S., Maruyama, J., Takeuchi, M., Kitamoto, K., Monitoring global gene expression of proteases and improvement of human lysozyme production in the nptB gene disruptant of Aspergillus oryzae. Biosci. Biotechnol. Biochem. 2008, 72, 499-505.
7. Teichert, U., Mechler, B., Müller, H., Wolf, D. H., Lysosomal (vacuolar) proteinases of yeast are essential catalysts for protein degradation, differentiation, and cell survival. J. Biol. Chem. 1989, 264, 16037-16045.
8. Jahic, M., Gustavsson, M., Jansen, A. K., Martinelle, M. et al., Analysis and control of proteolysis of a fusion protein in Pichia pastoris fed-batch processes. J. Biotechnol. 2003, 102, 45-53.
9. Ottow, K., Lütken-Petersen, T., Bukowska, M., Käppler, T. et al., Avoiding proteolysis during fermentation by using high gradient magnetic fishing. J. Biotechnol. 2007, 131, S169.
10. Käppler, T., Cerff, M., Ottow, K., Hobley, T. et al., In situ magnetic separation for extracellular protein production. Biotechnol. Bioeng. 2009, 102, 535-545.
11. Hubbuch, J. J., Thomas, O. R., High-gradient magnetic affinity separation of trypsin from porcine pancreatin. Biotechnol. Bioeng. 2002, 79, 301-313.
12. O'Brien, S. M., Thomas, O. R. T., Dunnill, P., Non-porous magnetic chelator supports for protein recovery by immobilised metal affinity adsorption. J. Biotechnol. 1996, 50, 13-25.
13. Mallia, A. K., Smith, P. K., Hermanson, G. T. (Ed.), Immobilized affinity ligand techniques. Academic Press, San Diego 1992.
14. Franzreb, M., Sieman-Herzber, M., Hobley, T. J., Thomas, O. R. T. et al., Product recovery by high-gradient magnetic fishing (HGMF), in Process Scale Bioseparations for the BIopharmaceutical Industry. CRC, Cambridge 2006, pp. 83-122.
15. Hubbuch, J. J., Matthiesen, D. B., Hobley, T. J., Thomas, O. R., High gradient magnetic separation versus expanded bed adsorption: a first principle comparison. Bioseparation 2001, 10, 99-112.
16. Meyer, A., Hansen, D. B., Gomes, C. S. G., Hobley, T. J. et al., Demonstration of a strategy for product purification by high-gradient magnetic fishing: Recovery of superoxide dismutase from unconditioned whey. Biotechnol. Prog. 2005, 21, 244-254.
17. Gomes, C. S. G., Advances in high-gradient magnetic fishing for bioprocessing. PhD thesis, Biocentrum-DTU, Technical University of Denmark, 2006, ISBN: 87-91494-50-8.
18. Langmuir, I., The adsorption of gases on plane surfaces of glass, mica and platinum. J. Am. Chem. Soc. 1918, 40, 1361-1403.
19. Hoffmann, C., Franzreb, M., Holl, W. H., A novel high-gradient magnetic separator (HGMS) design for biotech applications. IEEE Trans. Appl. Supercond. 2002, 12, 963-966.
20. Lütken-Petersen, P., New applications of high gradient magnetic fishing in bioprocesses of industrial relevance. PhD thesis, Department of Chemical Engineering, Technical University of Denmark, 2007, ISBN: 978-87-91435-55-2.
21. Ebner, N. A., Gomes, C. S. G., Hobley, T. J., Thomas, O. R. T. et al., Filter capacity predictions for the capture of magnetic microparticles by high-gradient magnetic separation. IEEE Trans. Magnetics 2007, 43, 1941-1949.
22. Fujimura, S., Shibata, Y., Nakamura, T., Purification and partial characterization of a lysine-specific protease of Porphyromonas gingivalis. FEMS Microbiol. Lett. 1993, 113, 133-137.
23. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K. et al., Measurement of protein using bicinchoninic acid. Anal. Biochem. 1985, 150, 76-85.
24. Scatchard, G., The Attractions of proteins for small molecules and ions. Ann. N. Y. Acad. Sci. 1949, 51, 660-672.
25. Schultz, N., Hobley, T. J., Syldatk, C., Spectrophotometric assay for online measurement of the activity of lipase immobilised on micro-magnetic particles. Biotechnol. Lett. 2007, 29, 365-371.
26. Stolarski, M., Keller, K., Eichholz, C., Fuchs, B., Nirschl, H., Continuous selective high gradient magnetic bio separation using novel rotation matrix centrifugation, in American filtration & Separation Society Annual Conference, May 19-22, Valley Forge, PA 2008.
27. Franzreb, M., Siemann-Herzberg, M., Hobley, T. J., Thomas, O.R.T., Protein purification using magnetic adsorbent particles. Appl. Microbiol. Biotechnol. 2006, 70, 505-516.