Magnetic nanoparticles: An improved method for mitochondrial isolation

Molecular Medicine Reports

Volumn 5, Issue 5, 2012, Pages 1271-1276

  Tang, Bo ^a   Zhao, Liang ^b   Liang, Rui ^a   Zhang, Yi ^a   Wang, Liming ^a  

^a DALIAN MEDICAL UNIVERSITY   (China)

^b DALIAN INSTITUTE OF CHEMICAL PHYSICS   (China)

Author keywords

Isolation; Magnetic nanoparticles; Mitochondria

Indexed keywords

AMINE OXIDASE (FLAVIN CONTAINING) ISOENZYME A; MAGNETIC NANOPARTICLE;

ARTICLE; ENZYME BINDING; ISOLATION PROCEDURE; MITOCHONDRION; RELIABILITY;

CELL FRACTIONATION; HELA CELLS; HEP G2 CELLS; HUMANS; MAGNETITE NANOPARTICLES; MITOCHONDRIA; MITOCHONDRIAL MEMBRANES; MONOAMINE OXIDASE;

EID: 84863286462     PISSN: 17912997     EISSN: 17913004     Source Type: Journal    
DOI: 10.3892/mmr.2012.806     Document Type: Article

References (24)

1. Lefort N, Glancy B, Bowen B, Willis WT, Bailowitz Z, De Filippis EA, Brophy C, Meyer C, Hojlund K, Yi Z and Mandarino LJ: Increased reactive oxygen species production and lower abundance of complex I subunits and carnitine palmitoyltransferase protein despite normal mitochondrial respiration in insulin-resistant human skeletal muscle. Diabetes 59: 2444-2452, 2010.
2. Kagan VE, Wipf P, Stoyanovsky D, Greenberger JS, Borisenko G, Belikova NA, Yanamala N, Samhan Arias AK, Tungekar MA, Jiang JF, Tyurina YY, Ji J, Klein-Seetharaman J, Pitt BR, Shvedova AA and Bayir H: Mitochondrial targeting of electron scavenging antioxidants: regulation of selective oxidation vs random chain reactions. Adv Drug Deliv Rev 61: 1375-1385, 2009.
3. 2+ channels. Circulation 119: 2435-2445, 2009.
4. Liu JH, Mao WK, Ding B and Liang CS: ERKs/p53 signal transduction pathway is involved in doxorubicin-induced apoptosis in H9c2 cells and cardiomyocytes. Am J Physiol Heart Circ Physiol 295: H1956-H1965, 2008.
5. Jiang X and Wang X: Cytochrome C-mediated apoptosis. Annu Rev Biochem 73: 87-106, 2004. (Pubitemid 39050364)
6. Sutherland LN, Capozzi LC, Turchinsky NJ, Bell RC and Wright DC: Time course of high-fat diet-induced reductions in adipose tissue mitochondrial proteins: potential mechanisms and the relationship to glucose intolerance. Am J Physiol Endocrinol Metab 295: E1076-E1083, 2008.
7. Wise DR, DeBerardinis RJ, Mancuso A, Sayed N, Zhang XY, Pfeiffer HK, Nissim I, Daikhin E, Yudkoff M, McMahon SB and Thompson CB: Myc regulates a transcriptional program that stimulates mitochondrial glutaminolysis and leads to glutamine addiction. Proc Natl Acad Sci USA 105: 18782-18787, 2008.
8. Kiebish MA, Han X, Cheng H, Chuang JH and Seyfried TN: Cardiolipin and electron transport chain abnormalities in mouse brain tumor mitochondria: lipidomic evidence supporting the Warburg theory of cancer. J Lipid Res 49: 2545-2556, 2008.
9. Petrosillo G, Fattoretti P, Matera M, Ruggiero FM, Bertoni-Freddari C and Paradies G: Melatonin prevents age-related mitochondrial dysfunction in rat brain via cardiolipin protection. Rejuvenation Res 11: 935-943, 2008.
10. Schapira AH: Mitochondrial dysfunction in neurodegenerative diseases. Neurochem Res 33: 2502-2509, 2008.
11. Wallace KB: Adriamycin-induced interference with cardiac mitochondrial calcium homeostasis. Cardiovasc Toxicol 7: 101-107, 2007.
12. Joza N, Susin SA, Daugas E, Stanford WL, Cho SK, Li CY, Sasaki T, Elia AJ, Cheng HY, Ravagnan L, Ferri KF, Zamzami N, Wakeham A, Hakem R, Yoshida H, Kong YY, Mak TW, Zuniga-Pflucker JC, Kroemer G and Penninger JM: Essential role of the mitochondrial apoptosis-inducing factor in programmed cell death. Nature 410: 549-554, 2001. (Pubitemid 32267191)
13. Liu Y, He J, Ji S, Wang Q, Pu H, Jiang T, Meng L, Yang X and Ji J: Comparative studies of early liver dysfunction in senescence-accelerated mouse using mitochondrial proteomics approaches. Mol Cell Proteomics 7: 1737-1747, 2008.
14. Mishra OP, Randis T, Ashraf QM and Delivoria-Papadopoulos M: Hypoxia-induced Bax and Bcl-2 protein expression, caspase-9 activation, DNA fragmentation, and lipid peroxidation in mitochondria of the cerebral cortex of newborn piglets: the role of nitric oxide. Neuroscience 141: 1339-1349, 2006. (Pubitemid 44137492)
15. Qin G, Wang Q, Liu J, Li B and Tian S: Proteomic analysis of changes in mitochondrial protein expression during fruit senescence. Proteomics 9: 4241-4253, 2009.
16. Sun Q, Miao M, Jia X, Guo W, Wang L, Yao Z, Liu C and Jiao B: Subproteomic analysis of the mitochondrial proteins in rats 24 h after partial hepatectomy. J Cell Biochem 105: 176-184, 2008.
17. Deng H, Li X, Peng Q, Wang X, Chen J and Li Y: Monodisperse magnetic single-crystal ferrite microspheres. Angew Chem Int Ed Engl 44: 2782-2785, 2005. (Pubitemid 40689587)
18. Wieckowski MR, Giorgi C, Lebiedzinska M, Duszynski J and Pinton P: Isolation of mitochondria-associated membranes and mitochondria from animal tissues and cells. Nat Protoc 4: 1582-1590, 2009.
19. Song Y, Hao Y, Sun A, Li T, Li W, Guo L, Yan Y, Geng C, Chen N, Zhong F, Wei H, Jiang Y and He F: Sample preparation project for the subcellular proteome of mouse liver. Proteomics 6: 5269-5277, 2006. (Pubitemid 44547165)
20. Estabrook RW: Mitochondrial respiratory control and the polarographic measurement of ADP: O ratios. Methods Enzymol 10: 41-47, 1967.
21. Batarseh A and Papadopoulos V: Regulation of translocator protein 18 kDa (TSPO) expression in health and disease states. Mol Cell Endocrinol 327: 1-12, 2010.
22. 2-induced cell apoptosis via preservation of mitochondria. Arch Biochem Biophys 464: 48-56, 2007.
23. Chaiyarit S and Thongboonkerd V: Comparative analyses of cell disruption methods for mitochondrial isolation in high-throughput proteomics study. Anal Biochem 394: 249-258, 2009.
24. Venkatakrishnan P, Nakayasu ES, Almeida IC and Miller RT: Absence of nitric-oxide synthase in sequentially purified rat liver mitochondria. J Biol Chem 284: 19843-19855, 2009.