Construction and identification of the recombinant adenovirus vector carrying a small interfering RNA targeting the peroxisome proliferator-activated receptor-γ

Chinese Medical Journal

Volumn 125, Issue 4, 2012, Pages 671-675

  Liu, Ming ^a   Wang, Yi Sheng ^a   Li, Yue Bai ^b   Zhao, Guo Qiang ^b  

^a THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIVERSITY   (China)

^b ZHENGZHOU UNIVERSITY   (China)

Author keywords

Adenovirus vector; Clone; Peroxisome proliferator activated receptor ; RNA interference

Indexed keywords

ADENOVIRUS VECTOR; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; SHORT HAIRPIN RNA; SMALL INTERFERING RNA;

ARTICLE; CELL STRAIN; CONTROLLED STUDY; DOWN REGULATION; DRUG INDUCED DISEASE; DRUG TARGETING; EXPRESSION VECTOR; FEMUR HEAD NECROSIS; GEL ELECTROPHORESIS; GENE FUNCTION; GENE IDENTIFICATION; GENE TARGETING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MARKER GENE; NONHUMAN; PPARGAMMA GENE; PROTEIN SYNTHESIS; PROTEIN TARGETING; RNA INTERFERENCE; RNA PURIFICATION; RNA SEQUENCE; SHUTTLE VECTOR; STEROID INDUCED FEMUR HEAD NECROSIS; VIRAL GENE DELIVERY SYSTEM; VIRUS IDENTIFICATION; VIRUS RECOMBINANT;

ADENOVIRIDAE; GENETIC VECTORS; PPAR GAMMA; RNA, SMALL INTERFERING;

EID: 84863164378     PISSN: 03666999     EISSN: None     Source Type: Journal    
DOI: 10.3760/cma.j.issn.0366-6999.2012.04.021     Document Type: Article

References (20)

1. Wang YS, Yin L, Li YB, Liu PL, Cui QJ. Preventive effects of puerarin on alcohol-induced osteonecrosis. Clin Orthop Relat Res 2008; 466: 1059-1067.
2. Jones JP Jr. Alcoholism, hypercortisonism, fat embolism and osseous avascular necrosis. Clin Orthop Relat Res 2001; (393): 4-12.
3. Cui QJ, Wang YS, Saleh KJ, Wang GJ, Balian G. Alcohol-induced adipogenesis in a cloned bone-marrow stem cell. J Bone Joint Surg Am 2006; 88: 148-154.
4. Wu XJ, Yin WL, Li YB, Wang YS. Effects of alcohol on mRNA expression of PPARγ and osteocalcin in human bone marrow mesenhymal stem cells. J Zhengzhou Univ (Med Sci) (Chin) 2006; 41: 1098-1100.
5. Marker DR, Seyler TM, McGrath MS, Delanois RE, Ulrich SD, Mont MA. Treatment of early stage osteonecrosis of the femoral head. J Bone Joint Surg Am 2008; 90: 175-187.
6. Li YB, Cao YW, Wu XJ, Wang YS. Steroid-induced regulation of gene involved in the osteogenesis and adipogenesis of human bone marrow mesenhymal stem cells. Chin J Exp Surg (Chin) 2006; 23: 1522-1523.
7. Wang YS, Wang XG, Li YB, Wang SH. Effects of RNAi for target on PPARγ to mainta in osteogenic differentiation of MSCs of rabbit suppressed by steroid. J Zhengzhou Univ (Med Sci) (Chin) 2009; 44: 275-278.
8. Wang YS, Li YB, Mao KY, Li J, Cui QJ, Wang GJ. Alcohol-induced adipogenesis in bone and marrow: a possible mechanism for osteonecrosis. Clin Orthop Relat Res 2003; (410): 213-224.
9. Mello CC, Conte D Jr. Revealing the world of RNA interference Nature 2004; 431: 338-342.
10. Wang YS, Wu XJ, Zhao GQ. Progression of RNA interference application. Henan Med Res (Chin) 2007; 16: 268-277.
11. Stephan AV, Kelly KH. Adenoviral gene therapy. Oncologist 2002; 7: 46-59.
12. Yin JQ, Wan Y. RNA-mediated gene regulation system: now and the future. Int J Mol Med 2002; 10: 355-365.
13. Wang YH, Liu S, Zhang G, Zhou CQ, Zhu HX, Zhou XB, et al. Knockdown of c-Myc expression by RNAi inhibits MCF-7 breast tumor cells growth in vitro and in vivo. Breast Cancer Res 2005; 7: 220-228.
14. Wang ZL, Li L. Adenovirus-mediated RNA interference against collagen-specific molecular chaperone 47-KDa heat shock protein suppresses scar formation on mouse wounds. Cell Biol Int 2008; 32: 480-483.
15. Heikkinen S, Auwerx J, Argmann CA. PPAR gamma in human and mouse physiology. Biochim Biophys Acta 2007; 1771: 999-1013.
16. Lehrke M, Lazar MA. The many faces of PPAR gamma. Cell 2005; 123: 993-999.
17. Zhao HY, Xia YY, Kang PD. Etiology and pathogenesis of osteonecrosis of the femoral head. Orthop J China (Chin) 2006; 17: 604-607.
18. Chan GK, Duque G. Age-related bone loss: old bone, new facts. Gerontology 2002; 48: 62-71.
19. Kirkland JL, Tchkonia T, Pirtskhalava T, Han J, Karagiannides I. Adipogenesis and aging: does aging make fat go MAD? Exp Gerontol 2002; 37: 757-767.
20. Yoon SY, Soo YL, Soo HL. Apoptosis in the osteonecrosis of the femoral head. Clin Orthop Surg 2010; 2: 250-255.