Amplification of 16S rDNA by nested PCR for measurement of mycoplasma pneumoniae DNA over time: Clinical application

Journal of Medical Microbiology

Volumn 61, Issue 3, 2012, Pages 426-430

  Han, Xiaohua ^a   Li, Shuxiu ^a   Lu, Sukun ^a   Liu, Liyun ^a   Li, Shuqin ^a   Zhang, Jihong ^a  

^a CHINA MEDICAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

AZITHROMYCIN; BACTERIAL DNA; BACTERIUM ANTIBODY; DNA 16S; ERYTHROMYCIN; IMMUNOGLOBULIN;

ADOLESCENT; ANTIBIOTIC RESISTANCE; ARTICLE; CHILD; CONTROLLED STUDY; EARLY DIAGNOSIS; FEMALE; GENE AMPLIFICATION; HUMAN; INFANT; LOWER RESPIRATORY TRACT INFECTION; MAJOR CLINICAL STUDY; MALE; MYCOPLASMA PNEUMONIA; MYCOPLASMA PNEUMONIAE; NONHUMAN; POLYMERASE CHAIN REACTION; PRESCHOOL CHILD; PRIORITY JOURNAL; RECURRENT DISEASE; SCHOOL CHILD; THROAT CULTURE; TREATMENT DURATION;

ADOLESCENT; BACTERIAL LOAD; CHILD; CHILD, PRESCHOOL; DNA, RIBOSOMAL; DRUG MONITORING; EARLY DIAGNOSIS; FEMALE; HUMANS; IMMUNOGLOBULINS, INTRAVENOUS; INFANT; MALE; MYCOPLASMA PNEUMONIAE; PNEUMONIA, MYCOPLASMA; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; TIME FACTORS; TREATMENT OUTCOME;

EID: 84863116758     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.030098-0     Document Type: Article

References (17)

1. Beersma, M. F., Dirven, K., van Dam, A. P., Templeton, K. E., Claas, E. C. & Goossens, H. (2005). Evaluation of 12 commercial tests and the complement fixation test for Mycoplasma pneumoniae-specific immu-noglobulin G (IgG) and IgM antibodies, with PCR used as the "gold standard". J Clin Microbiol 43, 2277-2285.
2. Daxboeck, F., Krause, R. & Wenisch, C. (2003). Laboratory diagnosis of Mycoplasma pneumoniae infection. Clin Microbiol Infect 9, 263-273.
3. Dumke, R. & Jacobs, E. (2009). Comparison of commercial and in-house real-time PCR assays used for detection of Mycoplasma pneumoniae. J Clin Microbiol 47, 441-444.
4. Gullsby, K., Storm, M. & Bondeson, K. (2008). Simultaneous detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae by use of molecular beacons in a duplex real-time PCR. J Clin Microbiol 46, 727-731.
5. Hou, A. C., Liu, Y. H., Xin, D. L., Li, J., Geng, B., Cui, H., Zhang, S. L., Xu, S. Z. & Zhao, G. C. (2002). Nasopharyngeal carriage status of common pathogenic microorganisms in healthy children and its clinical significance. Zhonghua Er Ke Za Zhi 40, 45-49 (in Chinese).
6. Lee, K. Y. (2008). Pediatric respiratory infections by Mycoplasma pneumoniae. Expert Rev Anti Infect Ther 6, 509-521.
7. McDonough, E. A., Barrozo, C. P., Russell, K. L. & Metzgar, D. (2005). A multiplex PCR for detection of Mycoplasma pneumoniae, Chlamy-dophila pneumoniae, Legionella pneumophila, and Bordetella pertussis in clinical specimens. Mol Cell Probes 19, 314-322.
8. Negi, V. S., Elluru, S., Sibéril, S., Graff-Dubois, S., Mouthon, L., Kazatchkine, M. D., Lacroix-Desmazes, S., Bayry, J. & Kaveri, S. V. (2007). Intravenous immunoglobulin: an update on the clinical use and mechanisms of action. J Clin Immunol 27, 233-245.
9. Nilsson, A. C., Björkman, P. & Persson, K. (2008). Polymerase chain reaction is superior to serology for the diagnosis of acute Mycoplasma pneumoniae infection and reveals a high rate of persistent infection. BMC Microbiol 8, 93.
10. Nour, M., Trabelsi, A., Maatouk, N. & Hammami, M. (2005). Amplification of P1 and 16S rRNA genes by nested PCR for detection of Myco-plasma pneumoniae in paediatric patients. Pathol Biol (Paris) 53, 9-14.
11. Okazaki, N., Ohya, H. & Sasaki, T. (2007). Mycoplasma pneumoniae isolated from patients with respiratory infection in Kanagawa Prefecture in 1976-2006: emergence of macrolide-resistant strains. Jpn J Infect Dis 60, 325-326.
12. Sung, H., Kang, S. H., Bae, Y. J., Hong, J. T., Chung, Y. B., Lee, C. K. & Song, S. (2006). PCR-based detection of Mycoplasma species. J Microbiol 44, 42-49.
13. Waites, K. B. & Talkington, D. F. (2004). Mycoplasma pneumoniae and its role as a human pathogen. Clin Microbiol Rev 17, 697-728.
14. Waites, K. B., Balish, M. F. & Atkinson, T. P. (2008). New insights into the pathogenesis and detection of Mycoplasma pneumoniae infections. Future Microbiol 3, 635-648.
15. Youn, Y. S., Lee, K. Y., Hwang, J. Y., Rhim, J. W., Kang, J. H., Lee, J. S. & Kim, J. C. (2010). Difference of clinical features in childhood Mycoplasma pneumoniae pneumonia. BMC Pediatr 10, 48.
16. Yuan, Z. & Xue, X. (2002). In Mycoplasma pneumoniae Encephalitis. Review of Diagnosis and Treatment of Critically Ill Pediatric Patients and Difficult Cases, pp. 256-257. Shenyang: People's Medical Publishing House.
17. Zhang, Z. R. (2004). Clinical Microbiology and Microbiological Examination, 3rd edn. Beijing: People's Medical Publishing House.