Reversible immobilization of K. fragilis β-galactosidase onto magnetic polyethylenimine-grafted nanospheres for synthesis of galacto-oligosaccharide

Journal of Molecular Catalysis B: Enzymatic

Volumn 82, Issue , 2012, Pages 64-70

  Liu, Jian Fu ^a,b   Liu, Hong ^c   Tan, Bin ^d   Chen, Yong Hong ^e   Yang, Rui Jin ^f  

^a TIANJIN UNIVERSITY OF COMMERCE   (China)

^b Tianjin Key Laboratory of Food Biotechnology   (China)

^c INSTITUTE OF PLANT PROTECTION   (China)

^d ACADEMY OF STATE ADMINISTRATION OF GRAIN   (China)

^e CHINA RURAL TECHNOLOGY DEVELOPMENT CENTER   (China)

^f JIANGNAN UNIVERSITY   (China)

Author keywords

Galacto oligosaccharide; Kluyveromyces fragilis galactosidase; Magnetic polyethylenimine grafted nanospheres; Reversible immobilization

Indexed keywords

ACTIVITY RECOVERY; ADSORBED ENZYME; ADSORPTION CAPACITIES; ADSORPTION-DESORPTION CYCLES; BATCH OPERATION; BATCH REACTIONS; GALACTO-OLIGOSACCHARIDE; GALACTOSIDASES; IMMOBILIZED BIOCATALYSTS; INITIAL ACTIVITY; KLUYVEROMYCES FRAGILIS; NANO-SIZED; OPERATIONAL STABILITY; POLYETHYLENEIMINE; REVERSIBLE IMMOBILIZATION; VIBRATING SAMPLE MAGNETOMETRY;

ACRYLIC MONOMERS; GRAFTING (CHEMICAL); NANOSPHERES; OLIGOSACCHARIDES; SCANNING ELECTRON MICROSCOPY;

ENZYME IMMOBILIZATION;

2 DIETHYLAMINOETHANOL; 2 HYDROXYETHYL METHACRYLATE; BETA GALACTOSIDASE; ETHYLENE GLYCOL DIMETHACRYLATE; GALACTOSE OLIGOSACCHARIDE; GLYCIDYL METHACRYLATE; METHACRYLIC ACID DERIVATIVE; NANOSPHERE; POLYETHYLENEIMINE; SEPHAROSE; UNCLASSIFIED DRUG;

ADSORPTION; ARTICLE; BIOCATALYST; CARBOHYDRATE SYNTHESIS; CATALYSIS; CONCENTRATION (PARAMETERS); DESORPTION; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ION EXCHANGE; IONIZATION; KLUYVEROMYCES MARXIANUS; MAGNETOMETRY; NONHUMAN; PH; SCANNING ELECTRON MICROSCOPY;

KLUYVEROMYCES MARXIANUS;

EID: 84863008786     PISSN: 13811177     EISSN: 18733158     Source Type: Journal    
DOI: 10.1016/j.molcatb.2012.06.001     Document Type: Article

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