Revised mechanism of complement lectin-pathway activation revealing the role of serine protease MASP-1 as the exclusive activator of MASP-2

Proceedings of the National Academy of Sciences of the United States of America

Volumn 109, Issue 26, 2012, Pages 10498-10503

  Héja, Dávid ^a   Kocsis, Andrea ^b   Dobó, József ^b   Szilágyi, Katalin ^b   Szász, Róbert ^c   Závodszky, Péter ^b   Pál, Gábor ^a   Gál, Péter ^a,b  

^a EÖTVÖS LORÁND UNIVERSITY   (Hungary)

^b INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^c UNIVERSITY OF DEBRECEN   (Hungary)

Author keywords

Canonical inhibitor; Complement system; Directed evolution; Innate immunity; Phage display

Indexed keywords

ALTERNATIVE COMPLEMENT PATHWAY C3 C5 CONVERTASE; COMPLEMENT COMPONENT C2A; LEPIRUDIN; MANNAN BINDING LECTIN ASSOCIATED SERINE PROTEINASE; MANNOSE BINDING LECTIN ASSOCIATED SERINE PROTEASE 1; MANNOSE BINDING LECTIN ASSOCIATED SERINE PROTEASE 2; SERINE PROTEINASE INHIBITOR; UNCLASSIFIED DRUG;

ARTICLE; COMPLEMENT LECTIN PATHWAY; CONTROLLED STUDY; ENZYME ACTIVATION; HUMAN; HUMAN EXPERIMENT; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; SERUM;

BLOOD COAGULATION; COMPLEMENT ACTIVATION; COMPLEMENT C3-C5 CONVERTASES; ENZYME ACTIVATION; HUMANS; LECTINS; MANNOSE-BINDING PROTEIN-ASSOCIATED SERINE PROTEASES;

EID: 84863001154     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1202588109     Document Type: Article

References (31)

1. Takeuchi O, Akira S (2010) Pattern recognition receptors and inflammation. Cell 140: 805-820.
2. Thiel S (2007) Complement activating soluble pattern recognition molecules with collagen-like regions, mannan-binding lectin, ficolins and associated proteins. Mol Immunol 44:3875-3888.
3. Dommett RM, Klein N, TurnerMW(2006) Mannose-binding lectin in innate immunity: Past, present and future. Tissue Antigens 68:193-209. (Pubitemid 44341793)
4. Endo Y, Matsushita M, Fujita T (2011) The role of ficolins in the lectin pathway of innate immunity. Int J Biochem Cell Biol 43:705-712.
5. Hansen S, et al. (2010) Collectin 11 (CL-11, CL-K1) is a MASP-1/3-associated plasma collectin with microbial-binding activity. J Immunol 185:6096-6104.
6. Gál P, Dobó J, Závodszky P, Sim RB (2009) Early complement proteases: C1r, C1s and MASPs. A structural insight into activation and functions. Mol Immunol 46:2745-2752.
7. Ikeda K, Sannoh T, Kawasaki N, Kawasaki T, Yamashina I (1987) Serum lectin with known structure activates complement through the classical pathway. J Biol Chem 262:7451-7454.
8. Degn SE, et al. (2009) MAp44, a human protein associated with pattern recognition molecules of the complement system and regulating the lectin pathway of complement activation. J Immunol 183:7371-7378.
9. Skjoedt MO, et al. (2010) A novel mannose-binding lectin/ficolin- associated protein is highly expressed in heart and skeletal muscle tissues and inhibits complement activation. J Biol Chem 285:8234-8243.
10. Stover CM, et al. (1999) Two constituents of the initiation complex of the mannanbinding lectin activation pathway of complement are encoded by a single structural gene. J Immunol 162:3481-3490. (Pubitemid 29313599)
11. Vorup-Jensen T, et al. (2000) Distinct pathways of mannan-binding lectin (MBL)- and C1-complex autoactivation revealed by reconstitution of MBL with recombinant MBL-associated serine protease-2. J Immunol 165:2093-2100. (Pubitemid 30643158)
12. Chen CB, Wallis R (2004) Two mechanisms for mannose-binding protein modulation of the activity of its associated serine proteases. J Biol Chem 279:26058-26065.
13. Ambrus G, et al. (2003) Natural substrates and inhibitors of mannan-binding lectin-associated serine protease-1 and -2: A study on recombinant catalytic fragments. J Immunol 170:1374-1382. (Pubitemid 36139504)
14. Gál P, et al. (2005) A true autoactivating enzyme. Structural insight into mannose-binding lectin-associated serine protease-2 activations. J Biol Chem 280:33435-33444. (Pubitemid 41397142)
15. Møller-Kristensen M, et al. (2003) Levels of mannan-binding lectin-associated serine protease-2 in healthy individuals. J Immunol Methods 282:159-167. (Pubitemid 37357041)
16. Møller-Kristensen M, Thiel S, Sjöholm A, Matsushita M, Jensenius JC (2007) Cooperation between MASP-1 and MASP-2 in the generation of C3 convertase through the MBL pathway. Int Immunol 19:141-149. (Pubitemid 46179009)
17. Schwaeble WJ, et al. (2011) Targeting of mannan-binding lectin-associated serine protease-2 confers protection from myocardial and gastrointestinal ischemia/reperfusion injury. Proc Natl Acad Sci USA 108:7523-7528.
18. Rossi V, et al. (2001) Substrate specificities of recombinant mannan-binding lectin-associated serine proteases-1 and -2. J Biol Chem 276:40880-40887.
19. Takahashi M, et al. (2008) Mannose-binding lectin (MBL)-associated serine protease (MASP)-1 contributes to activation of the lectin complement pathway. J Immunol 180: 6132-6138.
20. Héja D, et al. (2012) Monospecific inhibitors show that both mannan-binding lectin-associated serine protease (MASP)-1 and -2 are essential for lectin pathway activation and reveal structural plasticity of MASP-2. J Biol Chem 287:20290-20300.
21. Hein E, et al. (2010) Functional analysis of Ficolin-3 mediated complement activation. PLoS ONE 5:e15443.
22. Petersen SV, Thiel S, Jensen L, Steffensen R, Jensenius JC (2001) An assay for the mannan-binding lectin pathway of complement activation. J Immunol Methods 257: 107-116. (Pubitemid 33001238)
23. Kocsis A, et al. (2010) Selective inhibition of the lectin pathway of complement with phage display selected peptides against mannose-binding lectin-associated serine protease (MASP)-1 and -2: significant contribution of MASP-1 to lectin pathway activation. J Immunol 185:4169-4178.
24. Thiel S, et al. (2012) MASP-1, a serine protease associated with humoral pattern-recognition molecules: normal and acute-phase levels in serum and stoichiometry of lectin pathway components. Clin Exp Immunol 169:38-48.
25. Rawal N, Rajagopalan R, Salvi VP (2008) Activation of complement component C5: Comparison of C5 convertases of the lectin pathway and the classical pathway of complement. J Biol Chem 283:7853-7863.
26. Matsushita M (2010) Ficolins: complement-activating lectins involved in innate immunity. J Innate Immun 2:24-32.
27. Zhang M, et al. (2006) Identification of the target self-antigens in reperfusion injury. J Exp Med 203:141-152. (Pubitemid 43139653)
28. Jordan JE, Montalto MC, Stahl GL (2001) Inhibition of mannose-binding lectin reduces postischemic myocardial reperfusion injury. Circulation 104:1413-1418. (Pubitemid 32880155)
29. Ricklin D, Lambris JD (2007) Complement-targeted therapeutics. Nat Biotechnol 25: 1265-1275. (Pubitemid 350089828)
30. Seelen MA, et al. (2005) Functional analysis of the classical, alternative, and MBL pathways of the complement system: standardization and validation of a simple ELISA. J Immunol Methods 296:187-198. (Pubitemid 40174752)
31. Palarasah Y, et al. (2010) Sodium polyanethole sulfonate as an inhibitor of activation of complement function in blood culture systems. J Clin Microbiol 48:908-914.