Ectopic expression of a LEA protein gene TsLEA1 from Thellungiella salsuginea confers salt-tolerance in yeast and Arabidopsis

Molecular Biology Reports

Volumn 39, Issue 4, 2012, Pages 4627-4633

  Zhang, Yiyue ^a,b   Li, Yin ^a   Lai, Jianbin ^a,b   Zhang, Huawei ^b   Liu, Yuanyuan ^a   Liang, Liming ^a   Xie, Qi ^b  

^a SUN YAT SEN UNIVERSITY   (China)

^b INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY   (China)

Author keywords

LEA protein; Salt tolerance; Thellungiella salsuginea

Indexed keywords

ARABIDOPSIS LATE EMBRYOGENESIS ABUNDANT PROTEIN GROUP 4; ARABIDOPSIS LATE EMBRYOGENESIS ABUNDANT PROTEIN GROUP 5; COMPLEMENTARY DNA; THELLUNGIELLA SALSUGINEA LATE EMBRYOGENESIS ABUNDANT PROTEIN 1; UNCLASSIFIED DRUG; VEGETABLE PROTEIN; LATE EMBRYOGENESIS ABUNDANT PROTEIN, PLANT; SODIUM CHLORIDE;

AERIAL PART; AMINO ACID SEQUENCE; ARABIDOPSIS; ARTICLE; BRASSICACEAE; CELL SURVIVAL; CONTROLLED STUDY; DNA ISOLATION; DNA LIBRARY; GENE EXPRESSION; GENE OVEREXPRESSION; GENETIC MODEL; GENETIC TRANSCRIPTION; HALOPHYTE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PLANT GENE; PLANT ROOT; PLANT STRUCTURES; PROTEIN FUNCTION; SACCHAROMYCES CEREVISIAE; SALT STRESS; SALT TOLERANCE; SEEDLING; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SURVIVAL RATE; THELLUNGIELLA SALSUGINEA; THELLUNGIELLA SALSUGINEA LATE EMBRYOGENESIS ABUNDANT PROTEIN 1 GENE; TRANSGENIC PLANT; YEAST CELL; CHEMISTRY; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PHYSIOLOGICAL STRESS; PHYSIOLOGY;

ARABIDOPSIS; BRASSICACEAE; SACCHAROMYCES CEREVISIAE; THELLUNGIELLA; THELLUNGIELLA SALSUGINEA;

AMINO ACID SEQUENCE; ARABIDOPSIS; BRASSICACEAE; DNA, COMPLEMENTARY; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; MOLECULAR SEQUENCE DATA; PLANT PROTEINS; SACCHAROMYCES CEREVISIAE; SALT-TOLERANCE; SEEDLING; SODIUM CHLORIDE; STRESS, PHYSIOLOGICAL; TRANSCRIPTION, GENETIC;

EID: 84863000281     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-011-1254-8     Document Type: Article

References (33)

1. Bartels D, Salamini F (2001) Dessication tolerance in the resurrection plant Craterostigma plantagineum. A contribution to the study of drought tolerance at the molecular level. Plant Physiol 127:1346-1353
2. Manfre AJ, Lanni LM, Marcotte WR Jr. (2006) The Arabidopsis Group 1 late embryogenesis abundant protein ATEM6 is required for normal seed development. Plant Physiol 140:140-149
3. Hong-Bo S, Zong-Suo L, Ming-An S (2005) LEA proteins in higher plants: structure, function, gene expression and regulation. Colloids Surf B 45:131-135
4. Goyal K, Walton LJ, Tunnacliffe A (2005) LEA proteins prevent protein aggregation due to water stress. Biochem J 388:151-157
5. Lal S, Gulyani V, Khurana P (2008) Overexpression of HVA1 gene from barley generates tolerance to salinity and water stress in transgenic mulberry (Morus indica). Transgenic Res 17:651-663
6. Xiong L, Schumaker KS, Zhu JK (2002) Cell signaling during cold, drought, and salt stress. Plant Cell 14:S165-S183
7. Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247-273
8. Inan G, Zhang Q, Li P, Wang Z, Cao Z, Zhang H, Zhang C, Quist TM, Goodwin SM, Zhu J, Shi H, Damsz B, Charbaji T, Gong Q, Ma S, Fredricksen M, Galbraith DW, Jenks MA, Rhodes D, Hasegawa PM, Bohnert HJ, Joly RJ, Bressan RA, Zhu JK (2004) Salt cress. A halophyte and cryophyte Arabidopsis relative model system and its applicability to molecular genetic analyses of growth and development of extremophiles. Plant Physiol 135:1718-1737
9. Bressan RA, Zhang C, Zhang H, Hasegawa PM, Bohnert HJ, Zhu JK (2001) Learning from the Arabidopsis experience. The next gene search paradigm. Plant Physiol 127:1354-1360
10. Zhu JK (2001) Plant salt tolerance. Trends Plant Sci 6:66-71
11. Wang ZL, Li PH, Fredricksen M, Gong ZZ, Kim CS, Zhang CQ, Bohnert HJ, Zhu JK, Bressan RA, Hasegawa PM, Zhao YX, Zhang H (2004) Expressed sequence tags from Thellungiella halophila, a new model to study plant salt-tolerance. Plant Sci 166:609-616
12. Zhang YY, Lai JB, Sun SH, Yin Li, Liu YY, Liang LM, Chen MS, Xie Q (2008) Comparison analysis of transcripts from the halophyte Thellungiella halophila. J Integr Plant Biol 50(10):1327-1335
13. Quintero FJ, Garciadeblas B, Rodriguez-Navarro A (1996) The SAL1 gene of Arabidopsis, encoding an enzyme with 30(20),50-bisphosphate nucleotidase and inositol polyphosphate 1-phosphatase activities, increases salt tolerance in yeast. Plant Cell 8:529-537
14. Banuelos MA, Sychrova H, Bleykasten-Grosshans C, Souciet JL, Potier S (1998) The Nha1 antiporter of Saccharomyces cerevisiae mediates sodium and potassium efflux. Microbiology 144(10): 2749-2758
15. Amtmann A (2009) Learning from evolution: Thellungiella generates new knowledge on essential and critical components of abiotic stress tolerance in plants. Mol Plant 2(1):3-12
16. Shi H, Lee BH, Wu SJ, Zhu JK (2003) Overexpression of a plasma membrane Na+/H+ antiporter gene improves salt tolerance in Arabidopsis thaliana. Nat Biotechnol 21:81-85
17. Soni R, Murray A (1994) Isolation of intact DNA and RNA from plant tissue. Anal Biochem 218:474-476
18. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673-4680
19. Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM (2000) pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol Biol 42:819-832
20. Bent AF, Clough SJ (1998) Agrobacterium germ-line transformation: transformation of Arabidopsis without tissue culture. In: Gelvin SB, Schilperoot RA (eds) Plant molecular biology manual, 2nd edn. Kluwer Academic Publishers, Dordrecht, pp 1-14
21. Wong CE, Li Y, Whitty BR, Diaz-Camino C, Akhter SR, Brandle JE, Golding GB, Weretilnyk EA, Moffatt BA, Griffith M (2005) Expressed sequence tags from the Yukon ecotype of Thellungiella reveal that gene expression in response to cold, drought and salinity shows little overlap. Plant Mol Biol 58:561-574
22. Galloway GL, Malmberg RL, Price RA (1998) Phylogenetic utility of the nuclear gene arginine decarboxylase: an example from Brassicaceae. Mol Biol Evol 15:1312-1320
23. Battaglia M, Olvera-Carrillo Y, Garciarrubio A, Campos F, Covarrubias AA (2008) The enigmatic LEA proteins and other hydrophilins. Plant Physiol 148:6-24
24. Bies-Etheve N, Gaubier-Comella P, Debures A, Lasserre E, Raynal M, Jobet E, Cooke R, Delseny M (2008) Inventory, evolution and expression profiling diversity of the LEA (late embryogenesis abundant) protein gene family in Arabidopsis thaliana. Plant Mol Biol 67:107-124
25. Ingram J, Bartels D (1996) The molecular basis of dehydration tolerance in plants. Annu Rev Plant Physiol Plant Mol Biol 47:377-403
26. Shin D, Koo YD, Lee J, Lee HJ, Baek D, Lee S, Cheon CI, Kwak SS, Lee SY, Yun DJ (2004) Athb-12, a homeobox-leucine zipper domain protein from Arabidopsis thaliana, increases salt tolerance in yeast by regulating sodium exclusion. Biochem Biophys Res Commun 323:534-540
27. Chen AP, Wang GL, Qu ZL, Lu CX, Liu N, Wang F, Xia GX (2007) Ectopic expression of ThCYP1, a stress-responsive cyclophilin gene from Thellungiella halophila, confers salt tolerance in fission yeast and tobacco cells. Plant Cell Rep 26:237-245
28. Liu N, Chen AP, Zhong NQ, Wang F, Wang HY, Xia GX (2007) Functional screening of salt stress-related genes from Thellungiella halophila using fission yeast system. Physiol Plant 129:671-678
29. Xu D, Duan X, Wang B, Hong B, Ho T, Wu R (1996) Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiol 110:249-257
30. Mowla SB, Cuypers A, Driscoll SP, Kiddle G, Thomson J, Foyer CH, Theodoulou FL (2006) Yeast complementation reveals a role for an Arabidopsis thaliana late embryogenesis abundant (LEA)-like protein in oxidative stress tolerance. Plant J 48:743-756
31. Gilles GJ, Hines KM, Manfre AJ, Marcotte WR Jr. (2007) A predicted N-terminal helical domain of a Group 1 LEA protein is required for protection of enzyme activity from drying. Plant Physiol Biochem 45:389-399
32. Dalal M, Tayal D, Chinnusamy V, Bansal KC (2009) Abiotic stress and ABA-inducible Group 4 LEA from Brassica napus plays a key role in salt and drought tolerance. J Biotechnol 139:137-145
33. Olvera-Carrillo Y, Campos F, Reyes JL, Garciarrubio A, Covarrubias AA (2010) Functional analysis of the Group 4 late embryogenesis abundant proteins reveals their relevance in the adaptive response during water deficit in Arabidopsis. Plant Physiol 154:373-390