Secretory expression and characterization of a soluble laccase from the Ganoderma lucidum strain 7071-9 in Pichia pastoris

Molecular Biology Reports

Volumn 39, Issue 4, 2012, Pages 3807-3814

  Sun, Jing ^a,b   Peng, Ri He ^b   Xiong, Ai Sheng ^b   Tian, Yongsheng ^b   Zhao, Wei ^b   Xu, Hu ^a   Liu, Da Tong ^a   Chen, Jian Min ^a   Yao, Quan Hong ^b  

^a YANGZHOU UNIVERSITY   (China)

^b INSTITUTE OF EDIBLE FUNGI   (China)

Author keywords

Decolorization; Ganoderma lucidum; Laccase; Protein purification; Secretory expression

Indexed keywords

ALANINE; ALCOHOL OXIDASE; ARGININE; ASPARTIC ACID; COPPER ION; FERRIC ION; FERROUS ION; GLYCINE; HISTIDINE; LACCASE; MANNITOL; METHYL ORANGE; PHENYLALANINE; POTASSIUM ION; SODIUM AZIDE; SODIUM DITHIONITE; SODIUM ION; AZO COMPOUND; INORGANIC COMPOUND; ION; METAL; ORGANIC COMPOUND; RECOMBINANT PROTEIN;

ARTICLE; CODON; CODON USAGE; CONTROLLED STUDY; CULTURE MEDIUM; DECOLORIZATION; DEGRADATION; DNA SYNTHESIS; ENZYME ACTIVITY; ENZYME SUBSTRATE; FUNGAL GENE; FUNGAL STRAIN; GANODERMA LUCIDUM; GENE OVEREXPRESSION; LCCI GENE; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN EXPRESSION; TEMPERATURE; BIOREMEDIATION; COLOR; DRUG EFFECT; ENZYME STABILITY; ENZYMOLOGY; GENETIC SCREENING; GENETIC TRANSFORMATION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; PH; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; SECRETION; SOLUBILITY; TIME;

FUNGI; GANODERMA LUCIDUM; PICHIA PASTORIS;

AZO COMPOUNDS; BIODEGRADATION, ENVIRONMENTAL; CODON; COLOR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; GENES, FUNGAL; GENETIC TESTING; HYDROGEN-ION CONCENTRATION; INORGANIC CHEMICALS; IONS; KINETICS; LACCASE; METALS; ORGANIC CHEMICALS; PICHIA; RECOMBINANT PROTEINS; REISHI; SOLUBILITY; TEMPERATURE; TIME FACTORS; TRANSFORMATION, GENETIC;

EID: 84862989898     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-011-1158-7     Document Type: Article

References (28)

1. Soden DM, O'Callaghan J, Dobson AD (2002) Molecular cloning of a laccase isozyme gene from Pleurotus sajor-caju and expression in the heterologous Pichia pastoris host. Microbiology 148(Pt 12):4003-4014
2. Thurston CF (1994) The structure and function of fungal laccase. Microbiology 140:19-26
3. Prillinger H, Esser K (1975) The phenoloxidases of the ascomycete Podospora anserina XIII. Action and interaction of genes controlling the formation of laccase. Mol Gen Genet 156(3):333-345
4. Childs RE, Bardsley WG (1975) The steady-state kinetics of peroxidase with 2,20-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid) as chromogen. Biochem J 145(1):93-103
5. Murugesan K (2006) Decolorization of reactive dyes by a thermostable laccase produced by Ganoderma lucidum in solid state culture. Enzyme Microb Technol 40(2007):1662-1672
6. Jonsson LJ, Saloheimo M, Penttila M (1997) Laccase from the white-rot fungus Trametes versicolor: CDNA cloning of lcc1 and expression in Pichia pastoris. Curr Genet 32(6):425-430
7. Wang HX, Ng TB (2006) Purification of a laccase from fruiting bodies of the mushroom Pleurotus eryngii. Appl Microbiol Biotechnol 69(5):521-525. doi:10.1007/s00253-005-0086-7
8. Baldrian P (2006) Fungal laccases-occurrence and properties. FEMS Microbiol Rev 30(2):215-242. doi:10.1111/j.1574-4976. 2005.00010.x
9. Paterson RR (2006) Ganode-A therapeutic fungal biofactory. Phytochemistry 67(18):1985-2001. doi:10.1016/j.phytochem.2006. 07.004
10. Gao Y, Gao H, Chan E, Tang W, Xu A, Yang H, Huang M, Lan J, Li X, Duan W, Xu C, Zhou S (2005) Antitumor activity and underlying mechanisms of ganopoly, the refined polysaccharides extracted from Ganoderma lucidum, in mice. Immunol Invest 34(2):171-198
11. Hseu RS, Wang HH, Wang HF, Moncalvo JM (1996) Differentiation and grouping of isolates of the Ganoderma lucidum complex by random amplified polymorphic DNA-PCR compared with grouping on the basis of internal transcribed spacer sequences. Appl Environ Microbiol 62(4):1354-1363
12. Joo SS, Ryu IW, Park JK, Yoo YM, Lee DH, Hwang KW, Choi HT, Lim CJ, Lee do I, Kim K (2008) Molecular cloning and expression of a laccase from Ganoderma lucidum, and its antioxidative properties. Mol Cells 25(1):112-118
13. Ko EM, Leem YE, Choi HT (2001) Purification and characterization of laccase isozymes from the white-rot basidiomycete Ganoderma lucidum. Appl Microbiol Biotechnol 57(1-2):98-102
14. Cereghino JL, Cregg JM (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24(1):45-66
15. Xiong AS, Yao QH, Peng RH, Li X, Fan HQ, Cheng ZM, Li Y (2004) A simple, rapid, high-fidelity and cost-effective PCRbased two-step DNA synthesis method for long gene sequences. Nucleic Acids Res 32(12):e98. doi:10.1093/nar/gnh09432/ 12/e98
16. Nagai M, Sato T, Watanabe H, Saito K, Kawata M, Enei H (2002) Purification and characterization of an extracellular laccase from the edible mushroom Lentinula edodes, and decolorization of chemically different dyes. Appl Microbiol Biotechnol 60(3):327-335. doi:10.1007/s00253-002-1109-2
17. Biro JC (2008) Does codon bias have an evolutionary origin? Theor Biol Med Model 5:1-15
18. Kliman RM, Irving N, Santiago M (2003) Selection conflicts, gene expression, and codon usage trends in yeast. J Mol Evol 57(1):98-109. doi:10.1007/s00239-003-2459-9
19. Fu XY, Zhao W, Xiong AS, Tian YS, Peng RH (2010) High expression of recombinant Streptomyces sp. S38 xylanase in Pichia pastoris by codon optimization and analysis of its biochemical properties. Mol Biol Rep. doi:10.1007/s11033-010-0644-7
20. Cereghino GP, Cregg JM (1999) Applications of yeast in biotechnology: Protein production and genetic analysis. Curr Opin Biotechnol 10(5):422-427. doi:S0958-1669(99)00004-X
21. Peter MG, Wollenberger U (1997) Phenol-oxidizing enzymes: Mechanisms and applications in biosensors. EXS 80:63-82
22. Ullrich R, Huong le M, Dung NL, Hofrichter M (2005) Laccase from the medicinal mushroom Agaricus blazei: Production, purification and characterization. Appl Microbiol Biotechnol 67(3):357-363. doi:10.1007/s00253- 004-1861-6
23. Wang HX, Ng TB (2004) A novel laccase with fair thermostability from the edible wild mushroom (Albatrella dispansus). Biochem Biophys Res Commun 319(2):381-385. doi:10.1016/j. bbrc.2004.05.011
24. Wang HX, Ng TB (2004) A new laccase from dried fruiting bodies of the monkey head mushroom Hericium erinaceum. Biochem Biophys Res Commun 322(1):17-21. doi:10.1016/j.bbrc.2004. 07.075
25. Eggert C, Temp U, Eriksson KE (1996) The ligninolytic system of the white rot fungus Pycnoporus cinnabarinus: Purification and characterization of the laccase. Appl Environ Microbiol 62(4):1151-1158
26. Murugesan K, Kim YM, Jeon JR, Chang YS (2009) Effect of metal ions on reactive dye decolorization by laccase from Ganoderma lucidum. J Hazard Mater 168(1):523-529. doi: 10.1016/j.jhazmat.2009.02.075
27. Kiiskinen LL, Viikari L, Kruus K (2002) Purification and characterisation of a novel laccase from the ascomycete Melanocarpus albomyces. Appl Microbiol Biotechnol 59(2-3):198-204. doi:10.1007/s00253-002-1012-x
28. O'Callaghan J, O'Brien MM, McClean K, Dobson AD (2002) Optimisation of the expression of a Trametes versicolor laccase gene in Pichia pastoris. J Ind Microbiol Biotechnol 29(2):55-59. doi:10.1038/sj.jim.7000268