Affinity precipitation of active Rho-GEFs using a GST-tagged mutant Rho protein (GST-RhoA(G17A)) from epithelial cell lysates.

Journal of visualized experiments : JoVE

Volumn , Issue 61, 2012, Pages

  Waheed, Faiza ^a   Speight, Pamela ^b   Dan, Qinghong ^b   Garcia Mata, Rafael ^b   Szaszi, Katalin ^b  

^a ST MICHAEL'S HOSPITAL   (Canada)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

GLUTATHIONE TRANSFERASE; GUANINE NUCLEOTIDE EXCHANGE FACTOR; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN;

ARTICLE; CHEMISTRY; EPITHELIUM CELL; ESCHERICHIA COLI; GENETIC TRANSFORMATION; GENETICS; PRECIPITATION;

EPITHELIAL CELLS; ESCHERICHIA COLI; FRACTIONAL PRECIPITATION; GLUTATHIONE TRANSFERASE; GUANINE NUCLEOTIDE EXCHANGE FACTORS; RHOA GTP-BINDING PROTEIN; TRANSFORMATION, GENETIC;

EID: 84862988656     PISSN: None     EISSN: 1940087X     Source Type: Journal    
DOI: 10.3791/3932     Document Type: Article

References (0)