Pyrosequencing-based validation of a simple cell-suspension polymerase chain reaction assay for Campylobacter with application of high-processivity polymerase and novel internal amplification controls for rapid and specific detection

Diagnostic Microbiology and Infectious Disease

Volumn 72, Issue 2, 2012, Pages 131-138

  Oakley, Brian B ^a   Line, J Eric ^a   Berrang, Mark E ^a   Johnson, Jessica M ^b   Buhr, R Jeff ^a   Cox, Nelson A ^a   Hiett, Kelli L ^a   Seal, Bruce S ^a  

^a RUSSELL RESEARCH CENTER   (United States)

^b SOUTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

Campylobacter; Internal amplification control (IAC); Molecular diagnostics; Pyrosequencing

Indexed keywords

GENOMIC DNA; RNA 23S;

ARTICLE; BACTERIUM IDENTIFICATION; CAMPYLOBACTER; CELL SUSPENSION; COLONY FORMING UNIT; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; GENE AMPLIFICATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PYROSEQUENCING; SCREENING TEST; SENSITIVITY AND SPECIFICITY; VALIDATION PROCESS;

CAMPYLOBACTER; DNA-DIRECTED DNA POLYMERASE; PHYLOGENY; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

EID: 84862928533     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2011.11.001     Document Type: Article

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