Centrifugation assay for measuring adhesion of serially passaged bovine chondrocytes to polystyrene surfaces

Tissue Engineering - Part C: Methods

Volumn 18, Issue 7, 2012, Pages 537-544

  Kaplan, David S ^a   Hitchins, Victoria M ^a   Vegella, Thomas J ^a   Malinauskas, Richard A ^a   Ferlin, Kimberly M ^a,b   Fisher, John P ^b   Frondoza, Carmelita G ^c,d  

^a CENTER FOR DEVICES AND RADIOLOGICAL HEALTH   (United States)

^b UNIVERSITY OF MARYLAND   (United States)

^c NUTRAMAX LABORATORIES INC   (United States)

^d JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESION; BODY FLUIDS; CELL ADHESION; CELLS; CENTRIFUGATION; MAMMALS; MONOLAYERS; MORPHOLOGY; POLYSTYRENES;

ADHERENT CELLS; ADHESION PROPERTIES; BOVINE CHONDROCYTES; CARTILAGE REPAIR; CELL MORPHOLOGY; CELL NUMBER; CELL-ADHESION ASSAYS; CELLULAR ADHESION; CHONDROCYTES; COLLAGEN I; FIBROBLAST CELLS; FIBROBLASTIC CELLS; MONOLAYER CULTURE; MRNA EXPRESSION; PHENOTYPIC SHIFTS;

CELL CULTURE;

BOVINAE;

COLLAGEN TYPE 2; MESSENGER RNA; POLYSTYRENE DERIVATIVE;

ANIMAL; ARTICLE; CARTILAGE CELL; CATTLE; CELL ADHESION; CELL CULTURE; CELL DIFFERENTIATION; CELL PROLIFERATION; CENTRIFUGATION; CULTURE TECHNIQUE; CYTOLOGY; ENZYME IMMUNOASSAY; FIBROBLAST; GENETICS; METABOLISM; MOUSE; PHENOTYPE; PHYSIOLOGY; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS CULTURE; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CATTLE; CELL ADHESION; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL PROLIFERATION; CELLS, CULTURED; CENTRIFUGATION; CHONDROCYTES; COLLAGEN TYPE II; FIBROBLASTS; IMMUNOENZYME TECHNIQUES; MICE; PHENOTYPE; POLYSTYRENES; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SERIAL PASSAGE;

EID: 84862849704     PISSN: 19373384     EISSN: 19373392     Source Type: Journal    
DOI: 10.1089/ten.tec.2011.0043     Document Type: Article

References (40)

1. Mankin, H.J., et al. Chapter 9: Articular cartilage and osteoarthritis. In: Einhorn, T.A., O'Keefe, R.J., and Buckwalter, J.A., eds. Orthopaedic Basic Science: Foundations of Clinical Practice, third ed. Rosemont, IL: American Academy of Orthopaedic Surgeons, 2007, pp. 161-174.
2. Maroudas, A. Tissue composition and organization. In: Maroudas, A., and Kuettner, K., eds. Methods in Cartilage Research. London: Academic Press, 1990, pp. 209-239.
3. Gooch, K.J. et al. Chondrocytes: cartilage structure and function. In: Gooch, K.J., and Tennant, C.J., eds. Mechanical Forces: Their Effects on Cells and Tissues. Georgetown, TX: Landes Bioscience, 1997, pp. 79-100.
4. Mankin, H.J., et al. Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips: II. Correlation of morphology with biochemical and metabolic data. J Bone Joint Surg 53-A, 524, 1971.
5. Hungerford, D.S., et al. The rationale for cementless total hip replacement. Orthop Clin North Am 24, 617, 1993.
6. Lahiji, K., et al. Cyclic strain stimulates proliferative capacity, A2 integrin by human articular chondrocytes from osteoarthritic knee joints. UPOJ 15, 75, 2002.
7. Loeser, R.F. Molecular mechanisms of cartilage destruction: mechanics, inflammatory mediators and aging collide. Arthritis Rheum 54, 1357, 2006.
8. Peterson, L. Articular cartilage injuries treated with autologous chondrocyte transplantation in the human knee. Acta Orthop Belg 62(Suppl 1), 196, 1996.
9. Minas, T., and Peterson, L. Chondrocyte transplantation. Oper Tech Orthop 197, 323, 1997.
10. Brittberg, M., Lindahl, A., Nilsson, A., et al. Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. NEJM 331, 889, 1994.
11. Benya, P.D., and Shaffer, J.D. Dedifferentiated chondrocytes reexpress the differentiated phenotype when cultured in agarose. Cell 20, 215, 1982.
12. Ballock, R.T., et al. Thyroxine is the serum factor that regulates morphogenesis of columnar cartilage from isolated chondrocytes in chemically defined medium. J Cell Biol 126, 1311, 1994.
13. Hauselmann, H.J., et al. Phenotypic stability of bovine articular chondrocytes after long-term culture in alginate beads. J Cell Sci 107, 17, 1994.
14. Redini, F., et al. Differential expression of membraneanchored proteoglycans in rabbit articular chondrocytes cultured in monolayers and in alginate beads. Effect of transforming growth factor-beta 1. Biochim Biophys Acta 1355, 20, 1997.
15. Ishaug-Riley, S.L., et al. Human articular chondrocyte adhesion and proliferation on synthetic biodegradable polymer films. Biomaterials 20, 2245, 1999.
16. Mauck, R.L., et al. Chondrogenic differentiation and functional maturation of bovine mesenchymal stem cells in long-term agarose culture. Osteoarthritis Cartilage 2, 179, 2000.
17. Mercier, N.R., et al. Cartilage matrix assembly by chondrocytes seeded onto plg microspheres. Trans Ann Meet Orthop Res Soc 48(Section 1), 470, 2002.
18. Malda, J., et al. Expansion of bovine chondrocytes on microcarriers enhances redifferentiation. Tissue Eng 9, 939, 2003.
19. Schnabel, M., et al. Dedifferentiation-associated changes in morphology and gene expression in primary human articular chondrocytes in cell culture. Osteoarthritis Cartilage 10, 62, 2002.
20. Zlabinger, G.J., Menzel, J.E., and Steffen, C. Change in collagen synthesis of human chondrocyte culture. I. Development of a human model, demonstration of collagen type conversion by immunofluorescence. Rheumatol Int 6, 63, 1986.
21. Zlabinger, G.J., Menzel, J.E., and Steffen, C. Demonstration of collagen type conversion in culture supernatants from human chondrocytes by an inhibition ELISA. Clin Exp Rheumatol 6, 361, 1988.
22. Marlovits, S., Hombauer, M., Truppe, M., Vecsei, V., and Schleget, W. Changes in the ratio of type-1 and type-1II collagen expression during monolayer culture of human chondrocytes. J Bone Joint Sur (Br) 86-B, 286, 2004.
23. Yates, K., Allemann, F., and Glowacki, J. Phenotypic analysis of bovine chondrocytes cultured in 3D collagen sponges: effect of serum substitutes. Cell Tissue Bank 6, 45, 2005.
24. Reyes, C., and Garcia, A. A centrifugation cell adhesion assay for high-throughput screening of biomaterial surfaces. J. Biomed Mater Res 67A, 328, 2003.
25. Yoon, D.M., et al. Addition of hyaluronic acid to alginate embedded chondrocytes interferes with IGF-1 signaling in vitro and in vivo. Tissue Eng 15, 3449, 2009.
26. Gao, C., et al. CDK5 regulates cell adhesion and migration in corneal epithelial cells. Mol Cancer Res 1, 12, 2002.
27. Kurtis, M.S., et al. Integrin-mediated adhesion of human articular chondrocytes to cartilage. Arthritis Rheum 48, 110, 2003.
28. Jones, D.A., et al. P-Selectin mediates neutrophil rolling on histamine-stimulated endothelial cells. Biophys J 65, 1560, 1993.
29. Loeser, R.F., et al. Integrin expression by primary and immortalized human chondrocytes: evidence of a differential role for a1b1and a2b1 integrins in mediating chondrocyte adhesion to types II and VI collagen. Osteoarthritis Cartilage 8, 96, 2000.
30. Garcia, A.J., and Gallant, N.D. Stick and grip: measurement systems and quantitative analyses of integrin-mediated cell adhesion strength. Cell Biochem and Biophys 39, 61, 2003.
31. Garcia, A.J., et al. Integrin-fibronectin interactions at the cellmaterial interface: initial integrin binding and signaling. Biomaterials 20, 2427, 1999.
32. Garcia, A.J., et al. Modulation of cell proliferation and differentiation through substrate-dependent changes in fibronectin conformation. Mol Biol Cell 10, 785, 1999.
33. Gallant, N.D., et al. Micropatterned surfaces to engineer focal adhesions for analysis of cell adhesion strengthening. Langmuir 18, 5579, 2002.
34. Velegol, D., and Lanni, F. Cell traction forces on soft biomaterials. Microrheology of type I collagen gels. Biophys J 81, 1786, 2001.
35. Kino-Oka, M., et al. Morphological regulation of rabbit chondrocytes on glucose-displayed surface. Biomaterials 28, 1680, 2007.
36. Blaise, R., et al. Involvement of the notch pathway in the regulation of matrix metalloproteinase 13 and the dedifferentiation of articular chondrocytes in murine cartilage. Arthritis Rheum 60, 428, 2009.
37. Hoshiba, T., et al. Nuclear deformation and expression change of cartilaginous genes during in vitro expansion of chondrocytes. Biochem Biophy Res Commun 374, 688, 2008.
38. Kang, S.W., Yoo, S.P., and Kim, B.S. Effect of chondrocyte passage number on histological aspects of tissue-engineered cartilage. Biomed Mater Eng 17, 269-276, 2007. (Pubitemid 47357151)
39. Cheng, T., et al. Comparison of gene expression patterns in articular cartilage and dedifferentiated articular chondrocytes. J Orthop Res 30, 234, 2012.
40. McClay, D., and Hetzler, P. Quantitative measurement of cell adhesion using centrifugal force. Curr Protoc Cell Biol 9.2.1, 1998.