Leukocyte counting from a small amount of whole blood using a size-controlled microcavity array

Biotechnology and Bioengineering

Volumn 109, Issue 8, 2012, Pages 2017-2024

  Hosokawa, Masahito ^a   Asami, Marie ^a   Nakamura, Seita ^a   Yoshino, Tomoko ^a   Tsujimura, Noriyuki ^a   Takahashi, Masayuki ^b   Nakasono, Satoshi ^b   Tanaka, Tsuyoshi ^a   Matsunaga, Tadashi ^a  

^a TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

^b CENTRAL RESEARCH INSTITUTE OF ELECTRIC POWER INDUSTRY   (Japan)

Author keywords

CD4 CD8; Cell separation; Electroforming; Leukocyte; Microfluidic device; Whole blood

Indexed keywords

CD4/CD8; CELL SEPARATION; LEUKOCYTE; MICRO-FLUIDIC DEVICES; WHOLE BLOOD;

CENTRIFUGATION; DATA FLOW ANALYSIS; ELECTROFORMING; FLUIDIC DEVICES; MICROCAVITIES;

BLOOD;

ARTICLE; BLOOD; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CELL SEPARATION; DENSITY GRADIENT CENTRIFUGATION; HEMOLYSIS; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; LEUKOCYTE COUNT; MICROFLUIDICS; MONOCYTE; NEUTROPHIL;

CLINICAL LABORATORY TECHNIQUES; HUMANS; IMMUNOPHENOTYPING; LEUKOCYTE COUNT; MICROFLUIDIC ANALYTICAL TECHNIQUES; POINT-OF-CARE SYSTEMS;

EID: 84862824414     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24471     Document Type: Article

References (22)

1. Bruil A, Beugeling T, Feijen J, van Aken WG. 1995. The mechanisms of leukocyte removal by filtration. Transfus Med Rev 9: 145- 166.
2. Cheng X, Gupta A, Chen C, Tompkins RG, Rodriguez W, Toner M. 2009. Enhancing the performance of a point-of-care CD4+ T-cell counting microchip through monocyte depletion for HIV/AIDS diagnostics. Lab Chip 9: 1357- 1364.
3. Cook AM, Evans SA, Lane IF, Jones JG. 1998. Leucocyte filterability: Comparing diluted with undiluted blood. Br J Haematol 102: 952- 956.
4. George-Gay B, Parker K. 2003. Understanding the complete blood count with differential. J Perianesth Nurs 18: 96- 114.
5. Holmes D, Pettigrew D, Reccius CH, Gwyer JD, van Berkel C, Holloway J, Davies DE, Morgan H. 2009. Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry. Lab Chip 9: 2881- 2889.
6. Hosokawa M, Arakaki A, Takahashi M, Mori T, Takeyama H, Matsunaga T. 2009. High-density microcavity array for cell detection: Single-cell analysis of hematopoietic stem cells in peripheral blood mononuclear cells. Anal Chem 81: 5308- 5313.
7. Hosokawa M, Hayata T, Fukuda Y, Arakaki A, Yoshino T, Tanaka T, Matsunaga T. 2010. Size-selective microcavity array for rapid and efficient detection of circulating tumor cells. Anal Chem 82: 6629- 6635.
8. Ji HM, Samper V, Chen Y, Heng CK, Lim TM, Yobas L. 2008. Silicon-based microfilters for whole blood cell separation. Biomed Microdevices 10: 251- 257.
9. Matsunaga T, Hosokawa M, Arakaki A, Taguchi T, Mori T, Tanaka T, Takeyama H. 2008. High-efficiency single-cell entrapment and fluorescence in situ hybridization analysis using a poly(dimethylsiloxane) microfluidic device integrated with a black poly(ethylene terephthalate) micromesh. Anal Chem 80: 5139- 5145.
10. Mishra NN, Retterer S, Zieziulewicz TJ, Isaacson M, Szarowski D, Mousseau DE, Lawrence DA, Turner JN. 2005. On-chip micro-biosensor for the detection of human CD4(+) cells based on AC impedance and optical analysis. Biosens Bioelectron 21: 696- 704.
11. Panaro NJ, Lou XJ, Fortina P, Kricka LJ, Wilding P. 2005. Micropillar array chip for integrated white blood cell isolation and PCR. Biomol Eng 21: 157- 162.
12. Rodriguez WR, Christodoulides N, Floriano PN, Graham S, Mohanty S, Dixon M, Hsiang M, Peter T, Zavahir S, Thior I, Romanovicz D, Bernard B, Goodey AP, Walker BD, McDevitt JT. 2005. A microchip CD4 counting method for HIV monitoring in resource-poor settings. PLoS Med 2: e182.
13. Sethu P, Moldawer LL, Mindrinos MN, Scumpia PO, Tannahill CL, Wilhelmy J, Efron PA, Brownstein BH, Tompkins RG, Toner M. 2006. Microfluidic isolation of leukocytes from whole blood for phenotype and gene expression analysis. Anal Chem 78: 5453- 5461.
14. Shapiro HM, Schildkraut ER, Curbelo R, Laird CW, Turner B, Hirschfeld T. 1976. Combined blood cell counting and classification with fluorochrome stains and flow instrumentation. J Histochem Cytochem 24: 396- 401.
15. Stybayeva G, Mudanyali O, Seo S, Silangcruz J, Macal M, Ramanculov E, Dandekar S, Erlinger A, Ozcan A, Revzin A. 2010. Lensfree holographic imaging of antibody microarrays for high-throughput detection of leukocyte numbers and function. Anal Chem 82: 3736- 3744.
16. Toner M, Irimia D. 2005. Blood-on-a-chip. Annu Rev Biomed Eng 7: 77- 103.
17. VanDelinder V, Groisman A. 2007. Perfusion in microfluidic cross-flow: Separation of white blood cells from whole blood and exchange of medium in a continuous flow. Anal Chem 79: 2023- 2030.
18. Wang XB, Yang J, Huang Y, Vykoukal J, Becker FF, Gascoyne PR. 2000. Cell separation by dielectrophoretic field-flow-fractionation. Anal Chem 72: 832- 839.
19. Wang Z, Chin SY, Chin CD, Sarik J, Harper M, Justman J, Sia SK. 2010. Microfluidic CD4+ T-cell counting device using chemiluminescence-based detection. Anal Chem 82: 36- 40.
20. Wilding P, Kricka LJ, Cheng J, Hvichia G, Shoffner MA, Fortina P. 1998. Integrated cell isolation and polymerase chain reaction analysis using silicon microfilter chambers. Anal Biochem 257: 95- 100.
21. Wintrobe MM, Greer JP. 2009. Wintrobe's clinical hematology. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins.
22. Yager P, Edwards T, Fu E, Helton K, Nelson K, Tam MR, Weigl BH. 2006. Microfluidic diagnostic technologies for global public health. Nature 442: 412- 418.