Calcium imaging of multiple neurons in freely behaving C. elegans

Journal of Neuroscience Methods

Volumn 206, Issue 1, 2012, Pages 78-82

  Zheng, Maohua ^a   Cao, Pengxiu ^a   Yang, Jiong ^b   Xu, Shawn X Z ^d,e   Feng, Zhaoyang ^a,b,c  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b CASE WESTERN RESERVE UNIVERSITY   (United States)

^c XI'AN JIAOTONG UNIVERSITY   (China)

^d UNIVERSITY OF MICHIGAN   (United States)

^e UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

Behavior; C.elegans; Neuron

Indexed keywords

CALCIUM;

ACTION POTENTIAL; ANIMAL CELL; ARTICLE; BEHAVIOR; CAENORHABDITIS ELEGANS; CALCIUM RATIOMETRIC IMAGING OF BEHAVING NEMATODES VERSION II SYSTEM; CONTROLLED STUDY; DISSECTION; LOCOMOTION; NERVE CELL; NERVE CELL INHIBITION; NERVE CELL NETWORK; NEUROIMAGING; NONHUMAN; PRIORITY JOURNAL; SYSTEM ANALYSIS;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CAENORHABDITIS ELEGANS; CALCIUM SIGNALING; MOTOR ACTIVITY; NERVE NET; NEURONS; RANDOM ALLOCATION;

EID: 84862820669     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2012.01.002     Document Type: Article

References (19)

1. Alkema M.J., Hunter-Ensor M., Ringstad N., Horvitz H.R. Tyramine functions independently of octopamine in the Caenorhabditis elegans nervous system. Neuron 2005, 46:247-260.
2. Barr M.M. Super models. Physiol Genomics 2003, 13:15-24.
3. Ben Arous J., Tanizawa Y., Rabinowitch I., Chatenay D., Schafer W.R. Automated imaging of neuronal activity in freely behaving Caenorhabditis elegans. J Neurosci Methods 2010, 187:229-234.
4. Cao P., Yuan Y., Pehek E.A., Moise A.R., Huang Y., Palczewski K., et al. Alpha-synuclein disrupted dopamine homeostasis leads to dopaminergic neuron degeneration in Caenorhabditis elegans. PLoS One 2010, 5:e9312.
5. Chalasani S.H., Chronis N., Tsunozaki M., Gray J.M., Ramot D., Goodman M.B., et al. Dissecting a circuit for olfactory behaviour in Caenorhabditis elegans. Nature 2007, 450:63-70.
6. Chalfie M., Sulston J.E., White J.G., Southgate E., Thomson J.N., Brenner S. The neural circuit for touch sensitivity in Caenorhabditis elegans. J Neurosci 1985, 5:956-964.
7. Faumont S., Rondeau G., Thiele T.R., Lawton K.J., McCormick K.E., Sottile M., et al. An image-free opto-mechanical system for creating virtual environments and imaging neuronal activity in freely moving Caenorhabditis elegans. PLoS One 2011, 6:e24666.
8. Feng Z., Li W., Ward A., Piggott B.J., Larkspur E., Sternberg P.W., et al. elegans model of nicotine-dependent behavior: regulation by TRP family channels. Cell 2006, 127:621-633.
9. Gray J.M., Hill J.J., Bargmann C.I. A circuit for navigation in Caenorhabditis elegans. Proc Natl Acad Sci USA 2005, 102:3184-3191.
10. Kawano T., Po M.D., Gao S., Leung G., Ryu W.S., Zhen M. An imbalancing act: gap junctions reduce the backward motor circuit activity to bias C. elegans for forward locomotion. Neuron 2011, 72:572-586.
11. Kerr R., Lev-Ram V., Baird G., Vincent P., Tsien R.Y., Schafer W.R. Optical imaging of calcium transients in neurons and pharyngeal muscle of C. elegans. Neuron 2000, 26:583-594.
12. Liu J., Ward A., Gao J., Dong Y., Nishio N., Inada H., et al. C. elegans phototransduction requires a G protein-dependent cGMP pathway and a taste receptor homolog. Nat Neurosci 2010, 13:715-722.
13. Mori I., Okumura M., Kuhara A. Molecular neurogenetics of sensory behaviors in the hematode C. elgans. Nihon Shinkei Seishin Yakurigaku Zasshi 2004, 24:239-241.
14. Piggott B.J., Liu J., Feng Z., Wescott S.A., Xu X.Z.S. The neural circuits and synaptic mechanisms underlying motor initiation in C. elegans. Cell 2011, 147:922-933.
15. Ward A., Liu J., Feng Z., Xu X.Z. Light-sensitive neurons and channels mediate phototaxis in C. elegans. Nat Neurosci 2008, 11:916-922.
16. White J.G., Southgate E., Thomson J.N., Brenner S. The structure of the nervous system of the nematode Caenorhabditis elegans. Philos Trans R Soc Lond B 1986, 314:1-340.
17. Whittaker A.J., Sternberg P.W. Sensory processing by neural circuits in Caenorhabditis elegans. Curr Opin Neurobiol 2004, 14:450-456.
18. Zhang S., Jin W., Huang Y., Su W., Yang J., Feng Z. Profiling a Caenorhabditis elegans behavioral parametric dataset with a supervised K-means clustering algorithm identifies genetic networks regulating locomotion. J Neurosci Methods 2011, 197:315-323.
19. Zheng Y., Brockie P.J., Mellem J.E., Madsen D.M., Maricq A.V. Neuronal control of locomotion in C. elegans is modified by a dominant mutation in the GLR-1 ionotropic glutamate receptor. Neuron 1999, 24:347-361.