Radiotolerance of phosphatases of a Serratia sp.: Potential for the use of this organism in the biomineralization of wastes containing radionuclides

Biotechnology and Bioengineering

Volumn 109, Issue 8, 2012, Pages 1937-1946

  Paterson Beedle, M ^a   Jeong, B C ^b   Lee, C H ^c   Jee, K Y ^c   Kim, W H ^c   Renshaw, J C ^a   Macaskie, L E ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b Myong Ji University   (South Korea)

^c Korea Atomic Energy Research Institute   (South Korea)

Author keywords

Phosphatase; Radionuclides; Radiotoxicity; Serratia

Indexed keywords

ACTIVE SPECIES; BACTERIAL CELLS; CELL VIABILITY; COCRYSTALLIZATION; CONTAMINATED SOLUTIONS; CULTURABILITY; INITIAL ACTIVITY; MERCAPTOETHANOL; PHOSPHATASE ACTIVITY; RADIOTOXICITY; SERRATIA; SIMULATED WASTES; WHOLE CELL;

BIOMINERALIZATION; CELLS; CESIUM; CYTOLOGY; ENZYME ACTIVITY; FISSION PRODUCTS; HYDROGEN; NUCLEAR FUEL REPROCESSING; RADIOACTIVE WASTES; RADIOISOTOPES; SODIUM; URANIUM;

PHOSPHATASES;

CESIUM 137; COBALT 60; HYDROGEN URANYL PHOSPHATE; MERCAPTOETHANOL; NITRATE; PHOSPHATASE; PHOSPHATE; SODIUM ION; STRONTIUM 85; UNCLASSIFIED DRUG; URANIUM;

ARTICLE; BACTERIAL CELL; BIOFILM; BIOMINERALIZATION; BIOREACTOR; CELL VIABILITY; CONTROLLED STUDY; CRYSTALLIZATION; ENZYME ACTIVITY; GAMMA RADIATION; NONHUMAN; NUCLEAR FUEL REPROCESSING; RADIATION DOSE; RADIOACTIVE WASTE; RADIOACTIVE WASTE PROCESSING; RADIOSENSITIVITY; SERRATIA; WASTE COMPONENT REMOVAL; X RAY POWDER DIFFRACTION;

CRYSTALLIZATION; MERCAPTOETHANOL; MICROBIAL VIABILITY; PHOSPHORIC MONOESTER HYDROLASES; RADIATION-PROTECTIVE AGENTS; RADIOACTIVE WASTE; RADIOISOTOPES; SERRATIA; TIME FACTORS; WASTE DISPOSAL, FLUID; WASTE MANAGEMENT;

BACTERIA (MICROORGANISMS); SERRATIA; SERRATIA SP.;

EID: 84862814590     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24467     Document Type: Article

References (50)

1. Basnakova, G, Macaskie, LE. 1999. Accumulation of zirconium and nickel by CItrobacter sp. J. Chem. Technol. Biotechnol. 74: 509- 514.
2. Basnakova G, Stephens ER, Thaller MC, Rossolini GM, Macaskie LE. 1998. The use of E coli bearing a phoN gene for the removal of uranium and nickel from aqueous flows. Appl Microbiol Biotechnol 50: 266- 272.
3. Betti M, Aldave de las Heras L, Janssens A, Henrich E, Hunter G, Gerchikov M, Dutton M, van Weers AW, Nielsen S, Simmons J, Bexon A, Sazykina T. 2004. Results of the European Commission MARINA II study: Part I-General information and effects of discharges by the nuclear industry. J Environ Radioact 74: 243- 254.
4. Bradford MM. 1976. A rapid and sensitive method for the quantitation of micrograms quantities of protein using the principle of protein-dye binding. Anal Biochem 72: 248.
5. Choppin GR, Wong PJ. 1998. The chemistry of actinide behavior in marine systems. Aquatic Geochem 4: 77- 101.
6. Choppin GR, Bond AH, Hromadka PM. 1997. Redox speciation of plutonium. J Radioanal Nucl Chem 219: 203- 219.
7. Coggle JE. 1983. Biological effects of radiation. London: Taylor and Francis Ltd.
8. Deplanche K, Woods RD, Mikheenko IP, Sockett RE, Macaskie LE. 2008. Manufacture of stable palladium and gold nanoparticles on native and genetically engineered flagella scaffolds. Biotechnol Bioeng 101: 873- 880.
9. Finlay JA, Allan VJM, Conner A, Callow ME, Basnakova G, Macaskie LE. 1999. Phosphate release and heavy metal accumulation by biofilm-immobilized and chemically-coupled cells of a Citrobacter sp. pre-grown in continuous culture. Biotechnol Bioeng 63: 87- 97.
10. Gerchikov MY, van Weers A, Lepicard S, Dutton LMC, Bexon A, Buckley M. 2003. Civil nuclear discharges into North European waters. MARINA II, Annex A, European Commission.
11. Huey F, Hargis LG. 1967. Spectrophotometric determination of cesium using molybdophosphoric acid. Anal Chem 39: 125- 127.
12. Jeong BC. 1992. Studies on the atypical phosphatase of a metal accumulating Citrobacter sp. PhD thesis, University of Oxford, UK.
13. Jeong, BC, Macaskie, LE. 1999. Production of two phosphatases by a Citrobacter sp. grown in batch and continuous culture. Enzyme Microbial Technol. 24: 218- 224.
14. Jeong BC, Poole PS, Willis A, Macaskie LE. 1998. Purification and characterisation of acid-type phosphatases of a Citrobacter sp. Arch Microbiol 169: 166- 173.
15. Kelly H. 2002. FAS Testimony on terrorist nuclear threat. March 6, 2002. Available at
16. Kitayama S, Shih-Chen S, Matsuyama A. 1982. Effects of protein or polysaccharides on radiosensitization with NaCl. Agric Biol Chem 46: 571.
17. L'Annunziata MF. 1998. Handbook of Radioactivity Analysis. San Diego, CA: Academic Press.
18. Lindahl P, Roos P, Holm E, Dahlgaard H. 2005. Studies of Np and Pu in the marine environment of Swedish-Danish waters and the North Atlantic Ocean. J Environ Radioact 82: 285- 301.
19. Livens FR, Marsden OJ, Keith-Roach MJ, Morris K, Fifield LK, Day JP, Goodall PS. 2002. Geochemistry of artificial actinide isotopes in west Cumbria sediments. J Nucl Sci Technol Suppl 3: 939- 942.
20. Livesey JE, Reed DJ. 1987. Chemical protection against ionizing radiation. In: Lett JT, Ehmann UK, Cox AB, editors. Advances in radiation biology. New York: Academic Press. p 285- 340.
21. Lloyd JR, Cole JA, Macaskie LE. 1997. Reduction and removal of heptavalent technetium from solution by Escherichia coli J Bacteriol 179: 2014- 2021.
22. Lloyd JR, Ridley J, Khizniak T, Lyalikova NN, Macaskie LE. 1999a. Reduction of technetium by Desulfovibrio desulfuricans: Biocatalyst characterisation and use in a flow-through bioreactor. Appl Environ Microbiol 65: 2691- 2696.
23. Lloyd JR, Thomas GH, Finlay JA, Cole JA, Macaskie LE. 1999b. Microbial reduction of technetium by Escherichia coli and Desulfovibrio desulfuricans: Enhancement via the use of high activity strains and effect of process parameters. Biotechnol Bioeng 66: 122- 130.
24. Lloyd JR, Yong P, Macaskie LE. 2000. Biological reduction and removal of Np(V) by two microorganisms. Environ Sci Technol 34: 1297- 1301.
25. Lloyd JR, Pearce CI, Coker VS, Pattrick RAD, VanderLaan G, Cutting R, Vaughan DJ, Paterson-Beedle M, Mikheenko IP, Yong P, Macaskie LE. 2008. Biomineralization: Linking the fossil record to the production of high value functional materials. Geobiology 6: 285- 297.
26. Lowry OH, Rosenbrough HN, Farr AL, Randall RT. 1951. Protein measurement with the folin phenol reagent. J Biol Chem 193: 265.
27. Macaskie, LE. 1991. The application of biotechnology to the treatment of wastes produced from the Nuclear Fuel Cycle - biodegradation and bioaccumulation as a means of treating radionuclide-containing streams. Crit. Rev. Biotechnol. 11: 41- 112.
28. Macaskie LE, Basnakova G. 1998. Microbially-enhanced chemisorption of heavy metals (MECHM): A method for the bioremediation of solutions containing long-lived isotopes of neptunium and plutonium. Environ Sci Technol 32: 184- 187.
29. Macaskie LE, Dean ACR. 1985. Strontium accumulation by immobilized cells of a Citrobacter sp. Biotechnol Lett 7: 627- 630.
30. Macaskie LE, Lloyd JR. 2002. In: Keith-Roach MJ, Livens FR, editors Microbial interactions with radioactive wastes and potential applications. Interactions of microorganisms with radionuclides. Amsterdam: Elsevier Science. p 343- 381.
31. Macaskie LE, Jeong BC, Tolley MR. 1994. Enzymatically accelerated biomineralization of heavy metals-Application to the removal of americium and plutonium from aqueous flows. FEMS Microbiol Rev 14: 351- 367.
32. Macaskie LE, Lloyd JR, Thomas RAP, Tolley MR. 1996. The use of micro-organisms for the remediation of solutions contaminated with actinide elements, other radionuclides and organic contaminants generated by nuclear fuel cycle activities. Nucl Energy 35: 257- 271.
33. Macaskie LE, Yong P, Doyle TZ, Roig MG, Diaz M, Manzano T. 1997. Bioremediation of uranium-bearing wastewater: Biochemical and chemical factors affecting bioprocess application. Biotechnol Bioeng 53: 100- 109.
34. Medelia J. 2004. Nuclear terrorism: A brief review of threats and responses CRS Report for Congress code RL32595. Congressional Research Service, the Library of Congress, USA.
35. Mennan C, Paterson-Beedle M, Macaskie LE. 2010. Accumulation of zirconium phosphate by a Serratia sp.: A benign system for the removal of radionuclides from aqueous flows. Biotechnol Lett 32: 1419- 1427.
36. Michaylova V, Kouleva N. 1974. Spectrophotometric study of the reactions of arsenazo III with alkaline-earth metals. Talanta 21: 523- 532.
37. 99Tc concentrations in Fucus vesiculosus from the north-east Irish Sea. J Environ Radioact 77: 159- 173.
38. Nott PK, Heese F, Hall LD, Macaskie LE, Paterson-Beedle M. 2005. Measurement of flow field through biofilm reactors by three-dimensional magnetic resonance imaging. Am Inst Chem Eng 51: 3072- 3079.
39. Okada S. 1957. Inactivation of deoxyribonuclease by X-rays. II. The kinetics of inactivation in aqueous solution. Arch Biochem Biophys 67: 102.
40. Onishi H. 1986. Photometric determination of traces of metals. Part IIA: Individual metals, aluminium to lithium, 4th edition. John Wiley. New York: p 454- 459.
41. OSPAR Commission. 2004. Radioactive substances series: Liquid discharges from nuclear installations in 2002. ISBN 1-904426-42-5, p. 30.
42. Paridaens J, Vanmarcke H. 2001. Radium contamination of the banks of the river Laak as a consequence of the phosphate industry in Belgium. J Environ Radioact 54: 53- 60.
43. Paterson-Beedle M, Macaskie LE, Lee CH, Hriljac JA, Jee KY, Kim WH. 2006. Utilisation of a hydrogen uranyl phosphate based ion exchanger supported on a biofilm for the removal of cobalt, strontium and caesium from aqueous solutions. Hydrometallurgy 83: 141- 145.
44. Paterson-Beedle M, Macaskie LE, Readman JE, Hriljac JA. 2010. Biorecovery of uranium from minewaters into pure mineral product at the expense of phytic acid. Hydrometallurgy 194:524-528. 194: 524- 528.
45. Shih-Chen SJ, Kitayama S, Arai S, Masuda T, Matsuyama A. 1986. Mechanism of radiosensitizing effect of chloride on E. coli Int J Radiat Biol 49: 799- 808.
46. Stather JW, Smith H. 2002. Biological effects of ionising radiation. In: Martin CJ, Sutton DG, editors. Practical radiation protection in healthcare. Oxford: Oxford University Press. p 27- 42.
47. Yong P, Macaskie LE. 1997. The effect of substrate concentration and nitrate inhibition on phosphate release and heavy metal removal by a Citrobacter sp. Biotechnol Bioeng 55: 821- 830.
48. Yong P, Macaskie LE. 1999. The role of sulfate as a competitive inhibitor of enzymatically-mediated heavy metal uptake by Citrobacter sp.: Implications in the bioremediation of acid mine drainage water using biogenic phosphate precipitant. J Chem Technol Biotechnol 74: 1149- 1156.
49. Yong P, Eccles H, Macaskie LE. 1996. Determination of uranium, thorium and lanthanum in mixed solutions using simultaneous spectrophotometry. Anal Chim Acta 329: 173- 179.
50. Yong P, Macaskie LE, Sammons RL, Marquis PM. 2004. Synthesis of nanophase hydroxyapatite by a Serratia sp. from wastewater containing inorganic phosphate. Biotechnol Lett 26: 1723- 1730.