Phosphorylation of GIT1 tyrosine 321 is required for association with FAK at focal adhesions and for PDGF-activated migration of osteoblasts

Molecular and Cellular Biochemistry

Volumn 365, Issue 1-2, 2012, Pages 109-118

  Ren, Yongxin ^a   Yu, Lipeng ^a   Fan, Jin ^a   Rui, Ze ^a   Hua, Zhengzhe ^a   Zhang, Zitao ^a   Zhang, Ning ^a   Yin, Guoyong ^a  

^a NANJING MEDICAL UNIVERSITY   (China)

Author keywords

FAK; GIT1; Tyrosine phosphorylation

Indexed keywords

4 AMINO 7 TERT BUTYL 5 (4 CHLOROPHENYL)PYRAZOLO[3,4 D]PYRIMIDINE; FOCAL ADHESION KINASE; G PROTEIN COUPLED RECEPTOR KINASE; G PROTEIN COUPLED RECEPTOR KINASE INTERACTING PROTEIN 1; PLATELET DERIVED GROWTH FACTOR; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BONE MASS; CELL MIGRATION; CELL MOTILITY; CELL PROLIFERATION; FOCAL ADHESION; FRACTURE HEALING; MOUSE; MUTAGENESIS; NONHUMAN; OSTEOBLAST; PROTEIN PHOSPHORYLATION;

ANIMALS; CELL CYCLE PROTEINS; CELL MOVEMENT; CELL SIZE; CELLS, CULTURED; ENZYME ACTIVATION; FOCAL ADHESION KINASE 1; FOCAL ADHESIONS; GTPASE-ACTIVATING PROTEINS; MICE; MUTAGENESIS, SITE-DIRECTED; OSTEOBLASTS; PHOSPHORYLATION; PLATELET-DERIVED GROWTH FACTOR; PROTEIN BINDING; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN PROCESSING, POST-TRANSLATIONAL; SRC-FAMILY KINASES; TYROSINE;

EID: 84862811320     PISSN: 03008177     EISSN: 15734919     Source Type: Journal    
DOI: 10.1007/s11010-012-1249-3     Document Type: Article

References (28)

1. E Tsiridis N Upadhyay P Giannoudis 2007 Molecular aspects of fracture healing: which are the important molecular? Injury 38 suppl 1 S11 S25 17383481 10.1016/j.injury.2007.02.006
2. T Roelofsen R Akkers W Beumer M Apotheker I Steeghs J Van de Ven C Gelderblom A Garritsen K Dechering 2008 Sphingosine-1-phosphate acts as a developmental stage specific inhibitor of PDGF-induced chemotaxis of osteoblasts J Cell Biochem 105 1128 1138 18819098 10.1002/jcb.21915 1:CAS:528: DC%2BD1cXhtl2hsLfF
3. LC Gerstenfeld DM Cullinane GL Barnes, et al. 2003 Fracture healing as a post-natal developmental process: molecular, spatial, and temporal aspects of its regulation J Cell Biochem 88 873 884 12616527 10.1002/jcb.10435 1:CAS:528:DC%2BD3sXisVKktLs%3D
4. M Mehrotra SM Krane K Walters C Pilbeam 2004 Differential regulation of platelet-derived growth factor stimulated migration and proliferation in osteoblastic cells J Cell Biochem 93 741 752 15660418 10.1002/jcb.20138 1:CAS:528:DC%2BD2cXpvVCgtbY%3D
5. L Saldana N Vilaboa 2010 Effect of micrometric titanium particles on osteoblast attachment and cytoskeleton architecture Acta Biomater 6 1649 1660 19861182 10.1016/j.actbio.2009.10.033 1:CAS:528:DC%2BC3cXkvVOqtr8%3D
6. CM Bertolucci CD Guibao JJ Zheng 2008 Phosphorylation of paxillin LD4 destabilizes helix formation and inhibits binding to focal adhesion kinase Biochemistry 147 548 554 10.1021/bi702103n
7. R Hoefen B Berk 2006 The multifunctional GIT family of proteins J Cell Sci 119 1469 1475 16598076 10.1242/jcs.02925 1:CAS:528:DC%2BD28XlsFelsrk%3D
8. G Yin J Haendeler C Yan BC Berk 2004 GIT1 functions as a scaffold for MEK1-extracellular signal-regulated kinase 1 and 2 activation by angiotensin II and epidermal growth factor Mol Cell Biol 24 875 885 14701758 10.1128/MCB.24.2.875-885.2004 1:CAS:528:DC%2BD2cXktVyrsA%3D%3D
9. J Pang R Hoefen G Pryhuber J Wang G Yin R White X Xu M O'Dell A Mohan H Michaloski M Massett C Yan B Berk 2009 G-protein-coupled receptor kinase interacting protein-1 is required for pulmonary vascular development Circulation 119 1524 1532 19273721 10.1161/CIRCULATIONAHA.108.823997 1:CAS:528: DC%2BD1MXjt1SmsLg%3D
10. G Yin QL Zheng Chen Yan CBerk Bradford 2005 GIT1 is a scaffold for ERK1/2 activation in focal adhesion J Biol Chem 280 27705 27712 15923189 10.1074/jbc.M502271200 1:CAS:528:DC%2BD2MXmsVyqtL0%3D
11. P Menon G Yin EM Smolock MJ Zuscik C Yan BC Berk 2010 GPCR kinase 2 interacting protein 1 (GIT1) regulates osteoclast function and bone mass J Cell Physiol 225 77 85 10.1002/jcp.22282
12. Z Hu N Zhang G Yin 2007 Src activation required fir pERK1/2 activation in focal adhesions in osteoblasts induced by platelet-derived growth factor Zhongguo Xiu Fu chong Jian Wai Ke Za Zhi 21 1179 1183 18069470 1:CAS:528:DC%2BD1cXislOisbg%3D
13. D Ilic Y Furuta S Kanazawa N Take K Sobue N Nakatsuji, et al. 1995 Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice Nature 377 539 544 7566154 10.1038/377539a0 1:CAS:528:DyaK2MXoslWltrw%3D
14. T Sotobori T Ueda A Myoui K Yoshioka M Nakasaki H Yoshikawa K Itoh 2006 BMP promotes the hyptotactic migration of murine osteoblastic and osteosarcoma cells by enhancing incorporation of integrin beta 1 into lipid rafts Exp Cell Res 312 27 38 10.1016/j.yexcr.2006.08.024
15. GP Van Nieuw Amerongen K Natarajan G Yin 2004 GIT1 mediates thrombin signaling in endothelial cells: role in turnover of RhoA-type focal adhesion Cir Res 94 1041 1049 10.1161/01.RES.0000125627.77235.0C
16. Zeinab Daher Josette Noel Audrey Claing 2008 Endothelin-1 promotes migration of endothelial cells through the activation of Arf6 and the regulation of FAK activity Cell Signal 20 56 65 10.1016/j.cellsig.2008.08.021
17. MD Schaller JD Hildebrand JD Shannon JW Fox RR Vines JT Parsons 1994 Autophosphorylation of the focal adhesion kinase, pp 125FAK, directs SH2-dependent binding of pp60src Mol Cell Biol 14 1680 1688 7509446 1:CAS:528:DyaK2cXitl2jurg%3D
18. DD Schlaepfer SK Hanks T Hunter P van der Geer 1994 Integrin-mediated signal transduction linked to Ras pathway by GRB2 binding to focal adhesion kinase Nature 372 786 791 7997267 1:CAS:528:DyaK2MXivVSjsrg%3D
19. MC Brown LA Cary JS Jamieson JA Cooper CE Turner 2005 Src and FAK kinases cooperate to phosphorylate paxillin kinase linker, stimulate its focal adhesion localization, and regulate cell spreading and protrusiveness Mol Biol Cell 16 4316 4328 16000375 10.1091/mbc.E05-02-0131 1:CAS:528:DC%2BD2MXpvFKltr8%3D
20. Hu Zhang Ning YinGuoyong Zhiyi 2007 Mechanism of G protein coupled receptor kinase interacting protein 1 RNA hairpin inhibiting osteoblasts migration Chin J Reparative Reconstr Surg 21 1 5 1:STN:280: DC%2BD2s7gsFelug%3D%3D
21. G Tiscornia O Singer IM Verma, et al. 2006 Production and purification of lentiviral vectors J Nat Protoc 1 241 245 10.1038/nprot.2006.37 1:CAS:528:DC%2BD28XhtFOitbvP
22. C Walter A Pabst T Ziebart, et al. 2011 Bisphosphonates affect migration ability and cell viability of HUVEC, fibroblasts and osteoblasts in vitro Oral Dis 17 194 199 20796232 10.1111/j.1601-0825.2010.01720.x 1:STN:280: DC%2BC3M7ptVGhtg%3D%3D
23. RA Arkowitz N Lowe 1997 A small conserved domain in the yeast Spa2p is necessary and sufficient for its polarized localization J Cell Biol 138 17 36 9214378 10.1083/jcb.138.1.17 1:CAS:528:DyaK2sXksF2htbk%3D
24. DJ Webb MW Mayhew M Kovalenko MJ Schroeder ED Jeffery L Whitmore J Shabanowitz DF Hunt AF Horwitz 2006 Identification of phosphorylation sites in GIT1 J Cell Sci 15 2847 2850 10.1242/jcs03044
25. M Nakamura A Nagai Y Tanaka Y Sekijima K Yamashita 2010 Polarized hydroxyapatite promotes spread and motility of osteoblastic cells J Biomed Mater Res A 92 783 790 19274714
26. M Saura C Tarin C Zaragoza 2010 Recent insights into the implication of nitric oxide in osteoblast differentiation and proliferation during bone development Sci W J 13 624 632 10.1100/tsw.2010.58
27. J-B Kim P Leucht A Cynthia, et al. 2007 Reconciling the role of FAK in osteoblast differentiation, osteoclast remodeling, and bone regeneration Bone 41 39 51 17459803 10.1016/j.bone.2007.01.024 1:CAS:528:DC%2BD2sXmsFKku7o%3D
28. LB Smilenov A Mikhailov EE Marcantonio 1999 Focal adhesion motility revealed in stationary fibroblasts Science 286 1172 1174 10550057 10.1126/science.286.5442.1172 1:CAS:528:DyaK1MXnt1Krsbc%3D