Systematic prevention of bubble formation and accumulation for long-term culture of pancreatic islet cells in microfluidic device

Biomedical Microdevices

Volumn 14, Issue 2, 2012, Pages 419-426

  Wang, Yong ^a,b   Lee, Dongyoung ^a,b   Zhang, Lisa ^a   Jeon, Hyojin ^a   Mendoza Elias, Joshua E ^a,b   Harvat, Tricia A ^a   Hassan, Sarah Z ^a   Zhou, Amanda ^a   Eddington, David T ^b   Oberholzer, José ^a,b  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

^b University of Illinois at Chicago   (United States)

Author keywords

cells; Air bubble; Bubble trap; Cell culture; Islets of langerhans; Microfluidics

Indexed keywords

AIR BUBBLE FORMATION; AIR BUBBLES; BUBBLE REMOVAL; BUBBLE-TRAP; CELL VIABILITY; HYDROPHILIC SURFACES; INSULIN SECRETION; INTRACELLULAR CALCIUM; ISLETS OF LANGERHANS; LONG-TERM CULTURE; MICRO FLUIDIC SYSTEM; MICRO-FLUIDIC DEVICES; PANCREATIC ISLET; PANCREATIC ISLET CELLS;

BUBBLE FORMATION; FLUIDIC DEVICES; GLUCOSE; MICROFLUIDICS; SILICONES;

CELL CULTURE;

DIMETICONE; GLUCOSE; INSULIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL VIABILITY; HYDROPHILICITY; INSULIN RELEASE; MICROBUBBLE; MICROFLUIDIC ANALYSIS; MICROFLUIDICS; MITOCHONDRIAL MEMBRANE POTENTIAL; MORPHOLOGY; MOUSE; NONHUMAN; PANCREAS ISLET CELL; PRIORITY JOURNAL; RELIABILITY; VACUUM;

ANIMALS; CELL CULTURE TECHNIQUES; CELL SURVIVAL; DIMETHYLPOLYSILOXANES; EQUIPMENT DESIGN; GLUCOSE; INSULIN; ISLETS OF LANGERHANS; MICE; MICE, INBRED C57BL; MICROFLUIDIC ANALYTICAL TECHNIQUES; MICROFLUIDICS;

EID: 84862811310     PISSN: 13872176     EISSN: 15728781     Source Type: Journal    
DOI: 10.1007/s10544-011-9618-3     Document Type: Article

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