BDNF and NT-3 expression by using glucocorticoid-induced bicistronic expression vector pGC-BDNF-IRES-NT3 protects apoptotic cells in a cellular injury model

Brain Research

Volumn 1448, Issue , 2012, Pages 137-143

  Wang, Yongxiang ^a   Gu, Jiaxiang ^a   Wang, Jingcheng ^a   Feng, Xingmin ^a   Tao, Yuping ^a   Jiang, Baichuan ^a   He, Jinshan ^a   Wang, Qiang ^a   Yang, Jiandong ^a   Zhang, Shenfei ^a   Cai, Jun ^a   Sun, Yu ^a  

^a YANGZHOU UNIVERSITY   (China)

Author keywords

Apoptosis; BDNF; Bicistronic expression vector; Neurotrophin 3; Spinal cord injury

Indexed keywords

BRAIN DERIVED NEUROTROPHIC FACTOR; GLUCOCORTICOID; NEUROTROPHIN 3;

ANIMAL EXPERIMENT; ANIMAL MODEL; APOPTOSIS; ARTICLE; CELL DAMAGE; CELL PROTECTION; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; NERVE REGENERATION; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SPINAL CORD INJURY; WESTERN BLOTTING;

ANIMALS; APOPTOSIS; BLOTTING, WESTERN; BRAIN-DERIVED NEUROTROPHIC FACTOR; CELL LINE; CELLS, CULTURED; CYTOMEGALOVIRUS; DNA PRIMERS; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENETIC VECTORS; GLUCOCORTICOIDS; GREEN FLUORESCENT PROTEINS; INDICATORS AND REAGENTS; MICROSCOPY, FLUORESCENCE; MUTANT CHIMERIC PROTEINS; NEURONS; NEUROTROPHIN 3; PLASMIDS; RATS; RESPONSE ELEMENTS; SPINAL CORD; TRANSFECTION;

CYTOMEGALOVIRUS; EUKARYOTA; RATTUS;

EID: 84862796050     PISSN: 00068993     EISSN: 18726240     Source Type: Journal    
DOI: 10.1016/j.brainres.2012.02.007     Document Type: Article

References (13)

1. Allera-Moreau, C., Delluc-Clavieres, A., Castano, C., Van den Berghe, L., Golzio, M., Moreau, M., Teissie, J., Arnal, J.F., Prats, A.C., 2007. Long term expression of bicistronic vector driven by the FGF-1 IRES in mouse muscle. BMC Biotechnol. 7, 74.
2. Beato, M., Herrlich, P., Schutz, G., 1995. Steroid hormone receptors: many actors in search of a plot. Cell 83, 851-857.
3. Griesbeck, O., Parsadanian, A.S., Sendtner, M., Thoenen, H., 1995. Expression of neurotrophins in skeletal muscle: quantitative comparison and significance for motoneuron survival and maintenance of function. J. Neurosci. Res. 42, 21-33.
4. Hiebert, G.W., Khodarahmi, K., McGraw, J., Steeves, J.D., Tetzlaff, W., 2002. Brain-derived neurotrophic factor applied to the motor cortex promotes sprouting of corticospinal fibers but not regeneration into a peripheral nerve transplant. J. Neurosci. Res. 69, 160-168.
5. Ikeda, O., Murakami, M., Ino, H., Yamazaki, M., Nemoto, T., Koda, M., Nakayama, C., Moriya, H., 2001. Acute up-regulation of brain-derived neurotrophic factor expression resulting from experimentally induced injury in the rat spinal cord. Acta Neuropathol. 102, 239-245.
6. Ikeda, O., Murakami, M., Ino, H., Yamazaki, M., Koda, M., Nakayama, C., Moriya, H., 2002. Effects of brain-derived neurotrophic factor (BDNF) on compression-induced spinal cord injury: BDNF attenuates down-regulation of superoxide dismutase expression and promotes up-regulation of myelin basic protein expression. J. Neuropathol. Exp. Neurol. 61, 142-153.
7. Kraus, J.F., Franti, C.E., Riggins, R.S., Richards, D., Borhani, N.O., 1975. Incidence of traumatic spinal cord lesions. J. Chronic Dis. 28, 471-492.
8. Kwon, B.K., Liu, J., Messerer, C., Kobayashi, N.R., McGraw, J., Oschipok, L., Tetzlaff, W., 2002. Survival and regeneration of rubrospinal neurons 1 year after spinal cord injury. Proc. Natl. Acad. Sci. U. S. A. 99, 3246-3251.
9. Lamballe, F., Klein, R., Barbacid, M., 1991. trkC, a new member of the trk family of tyrosine protein kinases, is a receptor for neurotrophin-3. Cell 66, 967-979.
10. Novikova, L.N., Novikov, L.N., Kellerth, J.O., 2000. Survival effects of BDNF and NT-3 on axotomized rubrospinal neurons depend on the temporal pattern of neurotrophin administration. Eur. J. Neurosci. 12, 776-780.
11. Que, H., Liu, Y., Jia, Y., Liu, S., 2011. Establishment and assessment of a simple and easily reproducible incision model of spinal cord neuron cells in vitro. In Vitro Cell. Dev. Biol. Anim. 47, 558-564.
12. Shinoda, M., Hoffer, B.J., Olson, L., 1996. Interactions of neurotrophic factors GDNF and NT-3, but not BDNF, with the immune system following fetal spinal cord transplantation. Brain Res. 722, 153-167.
13. Tuszynski, M.H., Grill, R., Jones, L.L., Brant, A., Blesch, A., Low, K., Lacroix, S., Lu, P., 2003. NT-3 gene delivery elicits growth of chronically injured corticospinal axons and modestly improves functional deficits after chronic scar resection. Exp. Neurol. 181, 47-56.