Knockdown of Microplitis mediator Odorant Receptor Involved in the Sensitive Detection of Two Chemicals

Journal of Chemical Ecology

Volumn 38, Issue 3, 2012, Pages 287-294

  Li, Ke Ming ^a,b   Ren, Li Yan ^b   Zhang, Yong Jun ^b   Wu, Kong Ming ^b   Guo, Yu Yuan ^b  

^a NORTHWEST A F UNIVERSITY   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Braconidae; Electroantennogram; Hymenoptera; Microplitis mediator; Odorant receptor; Quantitative real time polymerase chain reaction (qPCR); Reverse transcription PCR (RT PCR) analysis; RNA interference (RNAi); Walking flight behavior; Y tube bioassay

Indexed keywords

ALDEHYDE; INSECT PROTEIN; MESSENGER RNA; NONANAL; PHEROMONE; SESQUITERPENE;

ATTRACTANT; BIOASSAY; FLIGHT BEHAVIOR; GENE EXPRESSION; GENETIC ANALYSIS; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; WASP;

ANIMAL; ANIMAL BEHAVIOR; ARTICLE; ELECTROPHYSIOLOGY; FEMALE; FLYING; GENETICS; MALE; METABOLISM; OLFACTORY RECEPTOR; PHYSIOLOGY; REAL TIME POLYMERASE CHAIN REACTION; RNA INTERFERENCE; WALKING; WASP;

ALDEHYDES; ANIMALS; BEHAVIOR, ANIMAL; ELECTROPHYSIOLOGICAL PHENOMENA; FEMALE; FLIGHT, ANIMAL; INSECT PROTEINS; MALE; PHEROMONES; REAL-TIME POLYMERASE CHAIN REACTION; RECEPTORS, ODORANT; RNA INTERFERENCE; RNA, MESSENGER; SESQUITERPENES; WALKING; WASPS;

BRACONIDAE; HEXAPODA; HYMENOPTERA; MICROPLITIS MEDIATOR;

EID: 84862793240     PISSN: 00980331     EISSN: 15731561     Source Type: Journal    
DOI: 10.1007/s10886-012-0085-y     Document Type: Article

References (41)

1. Amdam, G. V., Simo, Z. L., Guidugli, K. R., Norberg, K., and Omholt, S. W. 2003. Disruption of vitellogenin gene function in adult honeybees by intra-abdominal injection of double-stranded RNA. BMC Biotechnol. 3: 1.
2. Arthur, A. P. and Mason, P. G. 1986. Life history and immature stages of the parasitoid Microplitis mediator (Hymenoptera: Braconidae), reared on the bertha armyworm, Mamestra configurata (Lepidoptera: Noctuidae). Can. Entomol. 118: 487-491.
3. Benton, R., Saches, S., Michnick, S. W., and Vosshall, L. B. 2006. Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. PloS Biol. 4: 240-257.
4. Bettencourt, R., Terenius, O., and Faye, I. 2002. Hemolin gene silencing by ds-RNA injected into Cecropia pupae is lethal to next generation embryos. Insect Mol. Biol. 11: 267-271.
5. Boisson, B., Jacques, J. C., Choumet, V., Mattin, E., Xu, J., Vernick, K., and Bourgouin, C. 2006. Gene silencing in mosquito salivary glands by RNAi. FEBS Lett. 580: 1988-1992.
6. Bucher, G., Scholten, J., and Klingler, M. 2002. Parental RNAi in Tribolium (Coleoptera). Curr. Biol. 12: R85-R86.
7. Carthew, R. W. 2001. Gene silencing by double-stranded RNA. Curr. Opin. Cell Biol. 13: 244-248.
8. Clyne, P. J., Warr, C. G., Freeman, M. R., Lessing, D., and Kim, J. 1999. A novel family of divergent seven-transmembrane proteins: Candidate odorant receptors in Drosophila. Neuron 22: 327-338.
9. Dykxhoorn, D. M., Novina, C. D., and Sharp, P. A. 2003. Killing the messenger: short RNAs that silence gene expression. Nat. Rev. Mol. Cell Bio. 4: 457-467.
10. Eleftherianos, I., Marokhzal, J., Millichap, P. J., Hodgkinson, A. J., Sriboonlert, A., and Reynolds, S. E. 2006. Prior infection of Manduca sexta with non-pathogenic Escherichia coli elicits immunity to pathogenic Photo-rhabdus luminescens: roles of immune-related proteins shown by RNA interference. Insect Biochem. Mol. Biol. 36: 517-525.
11. Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., and Mello, C. C. 1998. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391: 806-811.
12. Gatehouse, H. S., Gatehouse, L. N., Malone, L. A., Hodges, S., Tregida, E., and Todd, J. 2004. Amylase activity in honey bee hypopharyngeal glands reduced by RNA interference. J. Apicult. Res. 43: 9-13.
13. Ghanim, M., Kontsedalov, S., and Czosnek, H. 2007. Tissue-specific gene silencing by RNA interference in the whitefly Bemisia tabaci (Gennadius). Insect Biochem. Mol. Biol. 37: 732-738.
14. Jones, W. D., Nguyen, T. A., Kloss, B., Lee, K. J., and Vosshall, L. B. 2005. Functional conservation of an insect odorant receptor gene across 250 million years of evolution. Curr. Biol. 15: R119-R121.
15. Kennerdell, J. R. and Carthew, R. W. 1998. Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway. Cell 95: 1017-1026.
16. Krieger, J., Grobe-Wilde, E., Gohl, T., and Breer, H. 2005. Candidate pheromone receptors of the silkmoth Bombyx mori. Eur. J. of Neurosci. 21: 2167-2176.
17. Krieger, J., Klink, O., Mohl, C., Raming, K., and Breer, H. 2003. A candidate olfactory receptor subtype highly conserved across different insect orders. J. Comp. Physiol. 189: 519-526.
18. Larsson, M. C., Domingos, A. I., Jones, W. D., Chiappe, M. E., and Amrein, H. 2004. Or83b encodes a broadly expressed odorant receptor essential for Drosophila olfaction. Neuron 43: 703-714.
19. Liu, S. -H., Ding, Z. -P., Zhang, C. -W., Yang, B. -J., and Liu, Z. -W. 2010. Gene knockdown by intro-thoracic injection of double-stranded RNA in the brown planthopper, Nilaparvata lugens. Insect Mol. Biol. 40: 666-671.
20. -ΔΔC Method. Methods. 25: 402-408.
21. Maleszka, J., Fore, S., Saint, R., and Maleszka, R. 2007. RNAi-induced phenotypes suggest a novel role for a chemosensory protein CSP5 in the development of embryonic integument in the honeybee (Apis mellifera). Dev. Genes Evol. 217: 189-196.
22. Meister, G. and Tuschl, T. 2004. Mechanisms of gene silencing by double-stranded RNA. Nature 431: 343-349.
23. Melo, A. C. A., Rutzler, M., Pitts, R. J., and Zwiebel, L. J. 2004. Identification of a chemosensory receptor from the yellow fever mosquito, Aedes aegypti, that is highly conserved and expressed in olfactory and gustatory organs. Chem. Senses 29: 403-410.
24. Misquitta, L. and Paterson, B. M. 1999. Targeted disruption of gene function in Drosophila by RNA interference (RNA-i): a role for nautilus in embryonic somatic muscle formation. Proc. Natl. Acad. Sci. USA. 96: 1451-1456.
25. Narasimhan, S., Montgomery, R. R., Deponte, K., Tschudi, C., Marcantonil, N., Anderson, J. F., Sauer, J. R., Cappello, M., Kantor, F. S., and Fikrig, E. 2004. Disruption of Ixodes scapularis anticoagulation by using RNA interference. Proc. Natl. Acad. Sci. USA. 101: 1141-1146.
26. Nichols, A. S. and Luetje, C. W. 2010. Transmembrane segment 3 of Drosophila melanogaster odorant receptor subunit 85b contributes to ligand-receptor interactions. J. Biol. Chem. 285: 11854-11862.
27. Pitts, R. J., Fox, A. N., and Zwiebel, L. J. 2004. A highly conserved candidate chemoreceptor expressed in both olfactory and gustatory tissues in the malaria vector Anopheles gambiae. Proc. Natl. Acad. Sci. USA. 101: 5058-5063.
28. Rajagopal, R., Sivakumar, S., Agrawal, N., Malhotra, P., and Bhatnagar, R. K. 2002. Silencing of midgut aminopeptidase N of Spodoptera litura by double-stranded RNA establishes its role as Bacillus thuringiensis toxin receptor. J. Biol. Chem. 277: 46849-46851.
29. Sato, K., Pellegrino, M., Nakagawa, T., Vosshall, L. B., and Touhara, K. 2008. Insect olfactory receptors are heteromeric ligand-gated ion channels. Nature 452: 1002-1006.
30. Shenefelt, R. D. 1973. Braconidae: Macrogasterinae and Ichneutinae, pp. 750-751, in V. V. Jones and R. D. Shenefelt (eds.). Hymenopterum Catalogus Nova Edito. W. Junk Press, Gravenhage, Holland.
31. Smart, R., Kiely, A., Beale, M., Vargas, E., and Carraher, C. 2008. Drosophila odorant receptors are novel seven transmembrane domain proteins that can signal independently of heterotrimeric G proteins. Insect Biochem. Molec. 38: 770-780.
32. Tomoyasu, Y. and Denell, R. E. 2004. Larval RNAi in Tribolium (Coleoptera) for analyzing adult development. Dev. Genes Evol. 214: 575-578.
33. Turner, C. T., Davy, M. W., Macdiarmid, R. M., Plummer, K. M., Brich, N. P., and Newcomb, R. D. 2006. RNA interference in the light brown apple moth, Epiphyas postvittana (Walker) induced by double-stranded RNA feeding. Insect Mol. Biol. 15: 383-391.
34. Vlachou, D., Schlegelmilch, T., Christophides, G. K., and Kafatos, F. C. 2005. Functional genomic analysis of midgut epithelial responses in Anopheles during Plasmodium invasion. Curr. Biol. 15: 1185-1195.
35. Vosshall, L. B., Amrein, H., Morozov, P. S., Rzhetsky, A., and Axel, R. 1999. A spatial map of olfactory receptor expression in the Drosophila antenna. Cell 96: 725-736.
36. Vosshall, L. B., Wong, A. M., and Axel, R. 2000. An olfactory sensory map in the fly brain. Cell 102: 147-159.
37. Wicher, D., Schafer, R., Bauernfeind, R., Stensmyr, M. C., and Heller, R. 2008. Drosophila odorant receptors are both ligand-gated and cyclic-nucleotide-activated cation channels. Nature 452: 1007-1011.
38. Yu, H. -L., Zhang, Y. -J., Kris, A. -G., Wu, K. -M., Gao, X. -W., and Guo, Y. -Y. 2010. Electrophysiological and behavioral responses of Microplitis mediator (Hymenoptera: Braconidae) to caterpillar-induced volatiles from cotton. Environ. Entomol. 39: 600-609.
39. Syed, Z., Kopp, A., Kimbrell, D. A., and Leal, W. S. 2010. Bombykol receptors in the silkworm moth and the fruit fly. Proc. Natl. Acad. Sci. USA. 107: 9436-9439.
40. Zhang, S., Zhang, Y. -J., Su, H. -H., Gao, X. -W., and Guo, Y. -Y. 2009. Gene cloning and tissue-specific expression of an olfactory receptor in Microplitis mediator (Hymenoptera: Braconidae). Scientia Agricultura Sinica 42: 1639-1645 (in Chinese).
41. Zhao, Y. -Y., Liu, F., Yang, G., and You, M. -S. 2010. PsOr1, a potential target for RNA interference-based pest management. Insect Mol. Biol. 20: 97-104.