Development of a one-step real-time RT-PCR assay using a minor-groove-binding probe for the detection of duck Tembusu virus

Journal of Virological Methods

Volumn 181, Issue 2, 2012, Pages 148-154

  Yun, Tao ^a   Ni, Zheng ^a   Hua, Jionggang ^a   Ye, Weicheng ^a   Chen, Liu ^a   Zhang, Shuai ^a,b   Zhang, Yan ^a   Zhang, Cun ^a  

^a INSTITUTE OF PLANT PROTECTION AND MICROBIOLOGY   (China)

^b SHANDONG AGRICULTURAL UNIVERSITY   (China)

Author keywords

Diagnostic assay; Duck Tembusu virus; Minor groove binding probe; Real time RT PCR

Indexed keywords

VIRUS RNA;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; ASSAY; CONTROLLED STUDY; DUCK; FLAVIVIRUS; MINOR GROOVE BINDING PROBE; MOLECULAR PROBE; NONHUMAN; PRIORITY JOURNAL; REAL TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SENSITIVITY AND SPECIFICITY; TEMBUSU VIRUS; VIRUS DETECTION; VIRUS GENOME; VIRUS LOAD;

ANIMALS; BIRD DISEASES; DUCKS; FLAVIVIRUS; FLAVIVIRUS INFECTIONS; OLIGONUCLEOTIDE PROBES; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TIME FACTORS; VIROLOGY;

TEMBUSU VIRUS;

EID: 84862779750     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2012.01.019     Document Type: Article

References (21)

1. Afonina I.A., Reed M.W., Lusby E., Shishkina I.G., Belousov Y.S. Minor groove binder-conjugated DNA probes for quantitative DNA detection by hybridization triggered fluorescence. Biotechniques 2002, 32:940-944.
2. Ala E.L., Russell E.B., John M., Leigh B.C., Penelope S., Susan A.N. Sensitive and specific detection of bovine immunodeficiency virus and bovine syncytial virus by 5' Taq nuclease assays with fluorescent 3' minor groove binder-DNA probes. J. Virol. Methods 2004, 115:167-175.
3. Barnard B.J., Du Preez J.H., Greyling S.P., Venter H.J. Turkey meningo encephalitis in South Africa. Onderstepoort J. Vet. Res. 1950, 47:89-94.
4. Belousov Y.S., Welch R.A., Sanders S., Mills A., Kulchenko A., Dempcy R., Afonina I.A., Walburger D.K., Glaser C.L., Yadavalli S., Vermeulen N.M., Mahoney W. Single nucleotide polymorphism genotyping by two color melting curve analysis using the MGB Eclipse Probe System in challenging sequence environment. Hum. Genomics 2004, 1:209-217.
5. Cao Z.Z., Zhang C., Liu Y.H., Ye W.C., Han J.W., Ma G.M., Zhang D.D., Xu F., Gao X.H., Tang Y., Shi S.H., Wan C.H., Zhang C., He B., Yang M.J., Lu X.H., Huang Y., Diao Y.X., Ma X.J., Zhang D.B. Emergence of Tembusu virus in ducks, China. Emerg. Infect. Dis. 2011, 17:1873-1875.
6. Caroline F., David B., Chaysavanh M., Nicole M., Henri A., Agnès G.D. Quantitation of HHV-7 genome by real-time polymerase chain reaction assay using MGB probe technology. J. Virol. Methods 2002, 106:11-16.
7. Fronhoffs S., Totzke G., Stier S., Wernert N., Rothe M., Bruning T., Koch B., Sachinidis A., Vetter H., Ko Y. A method for the rapid construction of cRNA standard curves in quantitative real-time reverse transcription polymerase chain reaction. Mol. Cell. Probes 2002, 16:99-110.
8. Islam A., Harrison B., Cheetham B.F., Mahony T.J., Young P.L., Walkden-Brown S.W. Differential amplification and quantitation of Marek's disease viruses using real-time polymerase chain reaction. J. Virol. Methods 2004, 119:103-113.
9. John M., Michael M., Bernt H., Francis M., Åse U., Carmina G., Brian A., Gordon A. Sensitive detection of African swine fever virus using real-time PCR with a 5' conjugated minor groove binder probe. J. Virol. Methods 2010, 168:141-146.
10. Kono Y., Tsukamoto K., Abd Hamid M., Darus A., Lian T.C., Sam L.S., Yok C.N., Di K.B., Lim K.T., Yamaguchi S., Narita M. Encephalitis and retarded growth of chicks caused by Sitiawan virus, a new isolate belong to the genus Flavivirus. Am. J. Trop. Med. Hyg. 2000, 63:94-101.
11. Kuno G., Chang G.J.J., Tsuchiya K.R., Karabatsos N., Cropp C.B. Phylogeny of the genus Flavivirus. J. Virol. 1998, 72:73-83.
12. Kutyavin I.V., Afonina I.A., Mills A., Gorn V., Lukhtanov E.A., Belousov E.S., Singer M.J., Walburger D.K., Lokhov S.G., Gall A.A., Dempcy R., Reed M.W., Meyer R.B., Hedgpeth J. 3'-Minor groove binder-DNA probes increase sequence specificity at PCR. Extension temperatures. Nucleic Acids Res. 2000, 28:655-661.
13. Liu L.H., Xia H.Y., Beláka S., Widén F. Development of a primer-probe energy transfer real-time PCR assay for improved detection of classical swine fever virus. J. Virol. Methods 2009, 160:69-73.
14. Liu S., Chen S., Li H., Kong X. Molecular characterization of the herpes simplex virus 1 (HSV-1) homologues, UL25 to UL30, in duck enteritis virus (DEV). Gene 2007, 401:88-96.
15. McMenamy M.J., McKillen J., Reid S.M., Hjertner B., King D.P., Adair B., Allan G. Development of a minor groove binder assay for real-time one-step RT-PCR detection of swine vesicular disease virus. J. Virol. Methods 2011, 171:219-224.
16. Mohamed F.A., Bruce D.H., Eva N., Leach R.R., Babak S., Spencer J.L., Shayan S. Development of a real-time PCR assay using SYBR Green chemistry for monitoring Marek's disease virus genome load in feather tips. J. Virol. Methods 2006, 133:34-40.
17. Sun Y.J., Yue Z.Q., Liu H., Zhao Y.R., Liang C.Z., Li Y., Shi X.J., Wu B., Xu B., Deng M.J., Zhu L.H., Wang Z. Development and evaluation of a sensitive and quantitative assay for hirame rhabdovirus based on quantitative RT-PCR. J. Virol. Methods 2010, 169:391-395.
18. Tang K.F.J., Wang J., Lightner D.V. Quantitation of Taura syndrome virus by real-time RT-PCR with a Taqman assay. J. Virol. Methods 2004, 115:109-114.
19. Yang M., Cheng A.C., Wang M.S., Xing H.Y. Development and application of a one-step real-time Taqman RT-PCR assay for detection of Duck hepatitis virus type1. J. Virol. Methods 2008, 153:55-60.
20. Yun T., Ye W.C., Ni Z., Zhang D.B., Zhang C. Identification and molecular characterization of a novel flavivirus isolated from Pekin ducklings in China. Vet. Microbiol. 2012, 10.1016/j.vetmic.2012.01.013.
21. Yun T., Zhang D.B., Ma X.J., Cao Z.Z., Chen L., Ni Z., Ye W.C., Yu B., Hua J.G., Zhang Y., Zhang C. Complete genome sequence of a novel Flavivirus, duck Tembusu virus, isolated from ducks and geese in China. J. Virol. 2012, 86(6). 10.1128/JVI.07132-11.