Novel standards in the measurement of rat insulin granules combining electron microscopy, high-content image analysis and in silico modelling

Diabetologia

Volumn 55, Issue 4, 2012, Pages 1013-1023

  Fava, E ^a,e   Dehghany, J ^b   Ouwendijk, J ^c,f   Muller A ^c   Niederlein, A ^a   Verkade, P ^a,f   Meyer Hermann, M ^b,d   Solimena, M ^a,c  

^a MAX PLANCK INSTITUTE OF MOLECULAR CELL BIOLOGY AND GENETICS   (Germany)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^c DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^d TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^e GERMAN CENTER FOR NEURODEGENERATIVE DISEASES DZNE   (Germany)

^f UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

Beta cell; Diabetes; High content analysis; High pressure freezing; In silico model; Insulin secretory granule; Transmission electron microscopy

Indexed keywords

INSULIN;

ANIMAL CELL; ARTICLE; CELL FUNCTION; CELL SIZE; CELL STRUCTURE; CELL VOLUME; COMPUTER MODEL; CONTROLLED STUDY; ELECTRON MICROSCOPY; EXOCYTOSIS; FEMALE; FREEZING; GRANULE DISTRIBUTION; GRANULE NUMBER; GRANULE SIZE; HIGH CONTENT IMAGE ANALYSIS; IMAGE ANALYSIS; INSULIN SECRETORY GRANULE; MEASUREMENT; NONHUMAN; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RAT; REPRODUCIBILITY; SECRETORY GRANULE; STANDARD; THREE DIMENSIONAL IMAGING;

ANIMALS; CELLS, CULTURED; CYTOPLASMIC GRANULES; FEMALE; INSULIN; INSULIN-SECRETING CELLS; MICROSCOPY, ELECTRON, TRANSMISSION; RATS; RATS, WISTAR; SECRETORY VESICLES;

EID: 84862576004     PISSN: 0012186X     EISSN: 14320428     Source Type: Journal    
DOI: 10.1007/s00125-011-2438-4     Document Type: Article

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