Identification of human fibroblast cell lines as a feeder layer for human corneal epithelial regeneration

PLoS ONE

Volumn 7, Issue 6, 2012, Pages

  Lu, Rong ^a,b   Bian, Fang ^b,c   Lin, Jing ^b,d   Su, Zhitao ^b   Qu, Yangluowa ^b   Pflugfelder, Stephen C ^b   Li, De Quan ^b  

^a SUN YAT SEN UNIVERSITY   (China)

^b CULLEN EYE INSTITUTE   (United States)

^c HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^d QINGDAO UNIVERSITY MEDICAL COLLEGE   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BETA1 INTEGRIN; CONNEXIN 43; CYTOKERATIN 12; CYTOKERATIN 3; EPIDERMAL GROWTH FACTOR RECEPTOR; PROTEIN P63;

ANIMAL CELL; ARTICLE; CELL EXPANSION; CELL GROWTH; CELL LINE; CELL STRUCTURE; COLONY FORMATION; CONTROLLED STUDY; CORNEA EPITHELIUM; CORNEA LIMBUS; DOWN REGULATION; EMBRYO; EPITHELIUM CELL; EX VIVO STUDY; FEEDER CELL; FETUS; FIBROBLAST; GENE CONTROL; GENE EXPRESSION PROFILING; HUMAN; HUMAN CELL; MALE; MOUSE; NEWBORN; NONHUMAN; NUCLEOTIDE SEQUENCE; PREPUCE; PROTEIN EXPRESSION; TISSUE REGENERATION; UPREGULATION;

3T3 CELLS; ANIMALS; BIOLOGICAL MARKERS; COCULTURE TECHNIQUES; EPITHELIAL CELLS; EPITHELIUM, CORNEAL; FEEDER CELLS; FIBROBLASTS; GENE EXPRESSION PROFILING; HUMANS; MICE; PHENOTYPE; REGENERATION; STROMAL CELLS;

EID: 84862492821     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0038825     Document Type: Article

References (37)

1. Dua HS, Saini JS, Azuara-Blanco A, Gupta P, (2000) Limbal stem cell deficiency: concept, aetiology, clinical presentation, diagnosis and management. Indian J Ophthalmol 48: 83-92.
2. Pellegrini G, Traverso CE, Franzi AT, Zingirian M, Cancedda R, et al. (1997) Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium. Lancet 349: 990-993.
3. Schwab IR, Reyes M, Isseroff RR, (2000) Successful transplantation of bioengineered tissue replacements in patients with ocular surface disease. Cornea 19: 421-426.
4. Tsai RJ, Li LM, Chen JK, (2000) Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells. N Engl J Med 343: 86-93.
5. Koizumi N, Inatomi T, Suzuki T, Sotozono C, Kinoshita S, (2001) Cultivated corneal epithelial stem cell transplantation in ocular surface disorders. Ophthalmology 108: 1569-1574.
6. Rama P, Bonini S, Lambiase A, Golisano O, Paterna P, et al. (2001) Autologous fibrin-cultured limbal stem cells permanently restore the corneal surface of patients with total limbal stem cell deficiency1. Transplantation 72: 1478-1485.
7. Kinoshita S, Koizumi N, Nakamura T, (2004) Transplantable cultivated mucosal epithelial sheet for ocular surface reconstruction. Exp Eye Res 78: 483-491.
8. Slavkin HC, Snead ML, Zeichner-David M, Jaskoll TF, Smith BT, (1984) Concepts of epithelial-mesenchymal interactions during development: tooth and lung organogenesis. J Cell Biochem 26: 117-125.
9. Sharpe PM, Ferguson MW, (1988) Mesenchymal influences on epithelial differentiation in developing systems. J Cell Sci pp. 195-230.
10. Cunha GR, Bigsby RM, Cooke PS, Sugimura Y, (1985) Stromal-epithelial interactions in adult organs. Cell Differ 17: 137-148.
11. Adamson IY, King GM, (1985) Epithelial-mesenchymal interactions in postnatal rat lung growth. Exp Lung Res 8: 261-274.
12. Howlett AR, Hodges GM, Rowlatt C, (1986) Epithelial-stromal interactions in the adult bladder: urothelial growth, differentiation, and maturation on culture facsimiles of bladder stroma. Dev Biol 118: 403-415.
13. Rheinwald JG, Green H, (1975) Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 6: 331-343.
14. Rheinwald JG, Green H, (1975) Formation of a keratinizing epithelium in culture by a cloned cell line derived from a teratoma. Cell 6: 317-330.
15. De Luca M, Cancedda R, (1992) Culture of human epithelium. Burns 18: S5-10.
16. Liu JY, Burg G, (2005) An improved organ culture for regeneration of pure autologous keratinocytes from small split-thickness skin specimens. Dermatology 210: 45-48.
17. van Rossum MM, Schalkwijk J, van de Kerkhof PC, van Erp PE, (2002) Immunofluorescent surface labelling, flow sorting and culturing of putative epidermal stem cells derived from small skin punch biopsies. J Immunol Methods 267: 109-117.
18. Sun T-T, Green H, (1977) Cultured epithelial cells of cornea, conjunctiva and skin: absence of marked intrinsic divergence of their differentiated states. Nature 269: 489-493.
19. Wei Z-G, Wu R-L, Lavker RM, Sun T-T, (1993) In vitro growth and differentiation of rabbit bulbar, fornix, and palpebral conjunctival epithelia. Implication on conjunctival epithelial transdifferentiation and stem cells. Invest Ophthalmol Vis Sci 34: 1814-1828.
20. Lindberg K, Brown ME, Chaves HV, Kenyon KR, Rheinwald JG, (1993) In vitro preparation of human ocular surface epithelial cells for transplantation. Invest Ophthalmol Vis Sci 34: 2672-2679.
21. Germain L, Auger FA, Grandbois E, Guignard R, Giasson M, et al. (1999) Reconstructed human cornea produced in vitro by tissue engineering. Pathobiology 67: 140-147.
22. Kim HS, Song XJ, de Paiva CS, Chen Z, Pflugfelder SC, et al. (2004) Phenotypic characterization of human corneal epithelial cells expanded ex vivo from limbal explant and single cell cultures. Exp Eye Res 79: 41-49.
23. Koizumi N, Cooper LJ, Fullwood NJ, Nakamura T, Inoki K, et al. (2002) An evaluation of cultivated corneal limbal epithelial cells, using cell-suspension culture. Invest Ophthalmol Vis Sci 43: 2114-2121.
24. Amit M, Margulets V, Segev H, Shariki K, Laevsky I, et al. (2003) Human feeder layers for human embryonic stem cells. Biol Reprod 68: 2150-2156.
25. Richards M, Tan S, Fong CY, Biswas A, Chan WK, et al. (2003) Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells. Stem Cells 21: 546-556.
26. Hovatta O, Mikkola M, Gertow K, Stromberg AM, Inzunza J, et al. (2003) A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells. Hum Reprod 18: 1404-1409.
27. Choo AB, Padmanabhan J, Chin AC, Oh SK, (2004) Expansion of pluripotent human embryonic stem cells on human feeders. Biotechnol Bioeng 88: 321-331.
28. Li D-Q, Tseng SC, (1995) Three patterns of cytokine expression potentially involved in epithelial-fibroblast interactions of human ocular surface. J Cell Physiol 163: 61-79.
29. Zipori D, (2004) Mesenchymal stem cells: harnessing cell plasticity to tissue and organ repair. Blood Cells Mol Dis 33: 211-215.
30. Tseng SC, Kruse FE, Merritt J, Li D-Q, (1996) Comparison between serum-free and fibroblast-cocultured single-cell clonal culture systems: evidence showing that epithelial anti-apoptotic activity is present in 3T3 fibroblast-conditioned media. Curr Eye Res 15: 973-984.
31. de Paiva CS, Chen Z, Corrales RM, Pflugfelder SC, Li D-Q, (2005) ABCG2 transporter identifies a population of clonogenic human limbal epithelial cells. Stem Cells 23: 63-73.
32. Tseng SC, Li D-Q, (1996) Comparison of protein kinase C subtype expression between normal and aniridic human ocular surfaces: implications for limbal stem cell dysfunction in aniridia. Cornea 15: 168-178.
33. Chen Z, de Paiva CS, Luo L, Kretzer FL, Pflugfelder SC, et al. (2004) Characterization of putative stem cell phenotype in human limbal epithelia. Stem Cells 22: 355-366.
34. Pellegrini G, Dellambra E, Golisano O, Martinelli E, Fantozzi I, et al. (2001) p63 identifies keratinocyte stem cells. Proc Natl Acad Sci U S A 98: 3156-3161.
35. Chen Z, Evans WH, Pflugfelder SC, Li D-Q, (2006) Gap junction protein connexin 43 serves as a negative marker for a stem cell-containing population of human limbal epithelial cells. Stem Cells 24: 1265-1273.
36. Dahlquist KD, Salomonis N, Vranizan K, Lawlor SC, Conklin BR, (2002) GenMAPP, a new tool for viewing and analyzing microarray data on biological pathways. Nat Genet 31: 19-20.
37. Doniger SW, Salomonis N, Dahlquist KD, Vranizan K, Lawlor SC, et al. (2003) MAPPFinder: using Gene Ontology and GenMAPP to create a global gene-expression profile from microarray data. Genome Biol 4: R7.