A microbiological profile of unexposed and exposed pulp space of primary endodontic infections by checkerboard DNA-DNA hybridization

Journal of Endodontics

Volumn 38, Issue 7, 2012, Pages 889-893

  Sassone, Luciana M ^a   Fidel, Rivail A ^a   Faveri, Marcelo ^b   Figueiredo, Luciene ^b   Fidel, Sandra R ^a   Feres, Magda ^b  

^a STATE UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b GUARULHOS UNIVERSITY   (Brazil)

Author keywords

Bacteria; clinical; DNA probes; endodontics; symptoms

Indexed keywords

BACTERIAL DNA;

ADOLESCENT; ADULT; AGED; ARTICLE; CHI SQUARE DISTRIBUTION; ENDODONTICS; FEMALE; GENETICS; HUMAN; MALE; METHODOLOGY; MICROBIOLOGY; MIDDLE AGED; MOLECULAR TYPING; NONPARAMETRIC TEST; NUCLEIC ACID HYBRIDIZATION; TOOTH PERIAPICAL DISEASE; TOOTH PULP; TOOTH PULP DISEASE;

ADOLESCENT; ADULT; AGED; CHI-SQUARE DISTRIBUTION; DENTAL PULP CAVITY; DENTAL PULP EXPOSURE; DENTAL PULP NECROSIS; DNA, BACTERIAL; FEMALE; HUMANS; MALE; MIDDLE AGED; MOLECULAR TYPING; NUCLEIC ACID HYBRIDIZATION; PERIAPICAL PERIODONTITIS; STATISTICS, NONPARAMETRIC; YOUNG ADULT;

EID: 84862293770     PISSN: 00992399     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.joen.2012.03.021     Document Type: Article

References (32)

1. W.D. Miller An introduction to the study of the bacterio-pathology of the dental pulp Dental Cosmos 36 1894 505 528
2. S. Kakehashi, H.R. Stanley, and R.J. Fitzgerald The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats Oral Surg Oral Med Oral Pathol 20 1965 340 349
3. G. Sundqvist Bacteriological Studies of Necrotic Dental Pulps (PhD Thesis) 1976 University of Umea Umea, Sweden
4. G. Sundqvist, E. Johansson, and U. Sjögren Prevalence of black-pigmented bacteroides species in root canal infections J Endod 15 1989 13 19
5. J.C. Baumgartner, and W.A. Falkler Bacteria in the apical 5 mm of infected root canals J Endod 17 1991 380 383
6. B.P.F.A. Gomes, J.D. Lilley, and D.B. Drucker Associations of endodontic symptoms and signs with particular combinations of specific bacteria Int Endod J 29 1996 69 75
7. I.Y. Jung, B.K. Choi, and K.Y. Kum Identification of oral spirochetes at the species level and their association with other bacteria in endodontic infections Oral Surg Oral Med Oral Pathol Oral Radiol Endod 92 2001 329 334
8. J.F. Siqueira Jr., I.N. Rôças, R. Souto, M. Uzeda, and A.P. Colombo Checkerboard DNA-DNA hybridization analysis of endodontic infections Oral Surg Oral Med Oral Pathol Oral Radiol Endod 89 2000 744 748
9. J.F. Siqueira Jr., I.N. Rôças, A. Favieri, J.C.M. Oliveira, and K.R.N. Santos Polymerase chain reaction detection of Treponema denticola in endodontic infections within root canal Int Endod J 34 2001 280 284
10. B.P.F.A. Gomes, R.C. Jacinto, and E.T. Pinheiro Molecular analysis of Filifactor alocis, Tannerella forsythia and Treponema denticola associated with primary endodontic infections and failed endodontic treatment J Endod 32 2006 937 940
11. J.F. Siqueira Jr., and I.N. Rôças Treponema socranskii in primary endodontic infections as detected by Nested PCR J Endod 29 2003 244 247
12. L. Sassone, R. Fidel, L. Figueiredo, S. Fidel, M. Faveri, and M. Feres Evaluation of the microbiota of primary endodontic infections using checkerboard DNA-DNA hybridization Oral Microbiol Immunol 22 2007 390 397
13. L. Sassone, R. Fidel, and M. Faveri A microbiological profile of symptomatic teeth with primary endodontic infections J Endod 34 2008 541 545
14. J.F. Siqueira Jr., and I.N. Rôças Bacteroides forsythus in primary endodontic infections as detected by Nested PCR J Endod 29 2003 390 393
15. C.A.S. Souza, R.P. Teles, R. Souto, M.A.E. Chaves, and A.P. Colombo Endodontic therapy associated with calcium hydroxide as na intracanal dressing: microbiological evaluation by the checkerboard DNA-DNA hybridization technique J Endod 31 2005 79 83
16. I.N. Rôças, F.R.F. Alves, A.L. Santos, A.S. Rosado, and J.F. Siqueira Jr. Apical root canal microbiota as determined by reverse-capture checkerboard analysis of cryogenically ground root samples from teeth with apical periodontitis J Endod 36 2010 1617 1621
17. C. Sedgley, A. Nagel, G. Dahlén, C. Reit, and A. Molander Real-time quantitative polymerase chain reaction and culture analyses of Enterococcus faecalis in root canals J Endod 32 2006 173 177
18. J.F. Siqueira Jr., and I.N. Rôças Detection of Filifactor alocis in endodontic infections associated with different forms of perirradicular diseases Oral Microbiol Immunol 18 2003 263 265
19. S.S. Socransky, C. Smith, L. Martin, B.J. Paster, F.E. Dewhirst, and A.E. Levin "Checkerboard" DNA-DNA hybridization Biotechniques 17 1994 788 792
20. G. Sundqvist Taxonomy, ecology, and pathogenicity of root canal flora Oral Surg Oral Med Oral Pathol 78 1994 522 530
21. G. Sundqvist Ecology of the root canal flora J Endod 18 1992 427 430
22. F.C.S. Chu, C.S.P. Tsang, T.W. Chow, and L.P. Samaranayake Identification of cultivable microorganisms from primary endodontic infections with exposed and unexposed pulp space J Endod 31 2005 424 429
23. A.D. Haffajee, M.A. Cugini, S. Dibart, C. Smith, R.L. Kent Jr., and S.S. Socransky Clinical and microbiological features of subjects with adult periodontitis who responded poorly to scaling and root planning J Clin Periodontol 24 1997 767 776
24. A.P. Feinberg, and B. Vogelstein A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity Anal Biochem 132 1983 6 13
25. M. Sakamoto, I.N. Rôças, J.F. Siqueira Jr., and Y. Benno Molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections Oral Microbiol Immunol 21 2006 112 122
26. J.F. Siqueira Jr., and I.N. Rôças Diversity of endodontic microbiota revisited J Dent Res 88 2009 969 981
27. A.C. Ribeiro, F. Matarazzo, M. Faveri, D.M. Zezell, and M.P.A. Mayer Exploring bacterial diversity of endodontic microbiota by cloning and sequencing 16S rRNA J Endod 37 2011 922 926
28. F. Montagner, B.P.F.A. Gomes, and P.S. Kumar Molecular fingerprinting reveals the presence of unique communities associated with paired samples of root canals and acute apical abscesses J Endod 36 2010 1475 1479
29. I.N. Rôças, J.F. Siqueira Jr., and G.J. Debelian Analysis of symptomatic and asymptomatic primary root canal infections in adult Norwegian patients J Endod 37 2011 1206 1212
30. L.R. Brown Jr., and C.E. Rodolph Jr. Isolation and identification of microorganism from exposed canals of pulp-involved teeth Oral Surg Oral Med Oral Pathol 10 1957 1094 1099
31. J.F. Siqueira Jr., I.N. Rôças, and A.S. Rosado Investigation of bacterial communities associated with asymptomatic and symptomatic endodontic infections by denaturing gradient gel electrophoresis fingerprinting approach Oral Microbiol Immunol 19 2004 363 370
32. J.F. Siqueira Jr., and I.N. Rôças Exploiting molecular methods to explore endodontic infections: part 1 - current molecular technologies for microbial diagnosis J Endod 31 2005 411 423