Evaluation of the cytopathicity (fusion/hemifusion) of patient-derived HIV-1 envelope glycoproteins comparing two effector cell lines

Journal of Biomolecular Screening

Volumn 17, Issue 6, 2012, Pages 727-737

  Cunyat, Francesc ^a   Curriu, Marta ^a   Marfil, Silvia ^a   García, Elisabet ^a   Clotet, Bonaventura ^a,b   Blanco, Julià ^a   Cabrera, Cecilia ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b HOSPITAL UNIVERSITARI GERMANS TRIAS I PUJOL   (Spain)

Author keywords

apoptosis; cytopathicity; envelope; fusion; hemifusion; HIV

Indexed keywords

VIRUS ENVELOPE PROTEIN;

ARTICLE; CELL ASSAY; CELL DEATH; CELL FUSION; CELL LOSS; CONTROLLED STUDY; CYTOPATHOLOGY; EFFECTOR CELL; HELA CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANTIBODIES, MONOCLONAL; ANTIGENS, CD4; CD4-POSITIVE T-LYMPHOCYTES; CELL FUSION; CYTOPATHOGENIC EFFECT, VIRAL; FLUORESCENT DYES; HEK293 CELLS; HELA CELLS; HIV ENVELOPE PROTEIN GP120; HIV ENVELOPE PROTEIN GP41; HIV-1; HUMANS; LUMINESCENT MEASUREMENTS; MEMBRANE FUSION; RECEPTORS, CXCR4;

EID: 84862206013     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057112439890     Document Type: Article

References (35)

1. + T Cells Go Out of Stock. Nat. Immunol. 2000 ; 1: 285-289
2. Douek D. C., Roederer M., Koup R. A.. Emerging Concepts in the Immunopathogenesis of AIDS. Annu. Rev. Med. 2009 ; 60: 471-484
3. Perfettini J. L., Castedo M., Roumier T., Andreau K., Nardacci R., Piacentini M., Kroemer G.. Mechanisms of Apoptosis Induction by the HIV-1 Envelope 2005. Cell Death Differ 12. (Suppl 1). 916-923
4. Gougeon M. L., Montagnier L.. Apoptosis in AIDS. Science. 1993 ; 260: 1269-1270
5. Doitsh G., Cavrois M., Lassen K. G., Zepeda O., Yang Z., Santiago M. L., Hebbeler A. M., Greene W. C.. Abortive HIV Infection Mediates CD4 T Cell Depletion and Inflammation in Human Lymphoid Tissue. Cell. 2010 ; 143: 789-801
6. Finkel T. H., Tudor-Williams G., Banda N. K., Cotton M. F., Curiel T., Monks C., Baba T. W., Ruprecht R. M., Kupfer A.. Apoptosis Occurs Predominantly in Bystander Cells and Not in Productively Infected Cells of HIV- and SIV-Infected Lymph Nodes. Nat. Med. 1995 ; 1: 129-134
7. Lifson J. D., Feinberg M. B., Reyes G. R., Rabin L., Banapour B., Chakrabarti S., Moss B., Wong-Staal F., Steimer K. S., Engleman E. G.. Induction of CD4-Dependent Cell Fusion by the HTLV-III/LAV Envelope Glycoprotein. Nature. 1986 ; 323: 725-728
8. Ferri K. F., Jacotot E., Blanco J., Esté J. A., Zamzami N., Susin S. A., Xie Z., Brothers G., Reed J. C., Penninger J. M., et al. Apoptosis Control in Syncytia Induced by the HIV Type 1-Envelope Glycoprotein Complex: Role of Mitochondria and Caspases. J. Exp. Med. 2000 ; 192: 1081-1092
9. Denizot M., Varbanov M., Espert L., Robert-Hebmann V., Sagnier S., Garcia E., Curriu M., Mamoun R., Blanco J., Biard-Piechaczyk M.. HIV-1 gp41 Fusogenic Function Triggers Autophagy in Uninfected Cells. Autophagy. 2008 ; 4: 998-1008
10. Blanco J., Barretina J., Clotet B., Esté J. A.. R5 HIV gp120-Mediated Cellular Contacts Induce the Death of Single CCR5-Expressing CD4 T Cells by a gp41-Dependent Mechanism. J. Leukoc. Biol. 2004 ; 76: 804-811
11. Blanco J., Barretina J., Ferri K. F., Jacotot E., Gutierrez A., Armand-Ugón M., Cabrera C., Kroemer G., Clotet B., Esté J. A.. Cell-Surface-Expressed HIV-1 Envelope Induces the Death of CD4 T Cells during GP41-Mediated Hemifusion-Like Events. Virology. 2003 ; 305: 318-329
12. Garg H., Joshi A., Freed E. O., Blumenthal R.. Site-Specific Mutations in HIV-1 gp41 Reveal a Correlation between HIV-1-Mediated Bystander Apoptosis and Fusion/Hemifusion. J. Biol. Chem. 2007 ; 282: 16899-16906
13. Kemble G. W., Danieli T., White J. M.. Lipid-Anchored Influenza Hemagglutinin Promotes Hemifusion, Not Complete Fusion. Cell. 1994 ; 76: 383-391
14. Bar S., Alizon M.. Role of the Ectodomain of the gp41 Transmembrane Envelope Protein of Human Immunodeficiency Virus Type 1 in Late Steps of the Membrane Fusion Process. J. Virol. 2004 ; 78: 811-820
15. Nussbaum O., Broder C. C., Berger E. A.. Fusogenic Mechanisms of Enveloped-Virus Glycoproteins Analyzed by a Novel Recombinant Vaccinia Virus-Based Assay Quantitating Cell Fusion-Dependent Reporter Gene Activation. J. Virol. 1994 ; 68: 5411-5422
16. Cheng D. C., Zhong G. C., Su J. X., Liu Y. H., Li Y., Wang J. Y., Hattori T., Ling H., Zhang F. M.. A Sensitive HIV-1 Envelope Induced Fusion Assay Identifies Fusion Enhancement of Thrombin. Biochem. Biophys. Res. Commun. 2011 ; 391: 1780-1784
17. Ji C., Zhang J., Cammack N., Sankuratri S.. Development of a Novel Dual CCR5-Dependent and CXCR4-Dependent Cell-Cell Fusion Assay System with Inducible gp160 Expression. J. Biomol. Screen. 2006 ; 11: 65-74
18. Sterjovski J., Churchill M. J., Ellett A., Gray L. R., Roche M. J., Dunfee R. L., Purcell D. F., Saksena N., Wang B., Sonza S.. Asn 362 in gp120 Contributes to Enhanced Fusogenicity by CCR5-Restricted HIV-1 Envelope Glycoprotein Variants from Patients with AIDS. Retrovirology. 2007 ; 4: 89
19. Gallo S. A., Sackett K., Rawat S. S., Shai Y., Blumenthal R.. The Stability of the Intact Envelope Glycoproteins Is a Major Determinant of Sensitivity of HIV/SIV to Peptidic Fusion Inhibitors. J. Mol. Biol. 2004 ; 340: 9-14
20. De Clercq E., Yamamoto N., Pauwels R., Balzarini J., Witvrouw M., De Vreese K., Debyser Z., Rosenwirth B., Peichl P., Datema R., et al. Highly Potent and Selective Inhibition of Human Immunodeficiency Virus by the Bicyclam Derivative JM3100. Antimicrob. Agents Chemother. 1994 ; 38: 668-674
21. Burton D. R., Pyati J., Koduri R., Sharp S. J., Thornton G. B., Parren P. W., Sawyer L. S., Hendry R. M., Dunlop N., Nara P. L., et al. Efficient Neutralization of Primary Isolates of HIV-1 by a Recombinant Human Monoclonal Antibody. Science. 1994 ; 266: 1024-1027
22. Malim M. H., Hauber J., Fenrick R., Cullen B. R.. Immunodeficiency Virus rev Trans-Activator Modulates the Expression of the Viral Regulatory Genes. Nature. 1988 ; 335: 181-183
23. Freed E. O., Delwart E. L., Buchschacher G. L., Panganiban A. T.. A Mutation in the Human Immunodeficiency Virus Type 1 Transmembrane Glycoprotein gp41 Dominantly Interferes with Fusion and Infectivity. Proc. Natl. Acad. Sci. U. S. A. 1992 ; 89: 70-74
24. Garg H., Joshi A., Blumenthal R.. Altered Bystander Apoptosis Induction and Pathogenesis of Enfuvirtide-Resistant HIV Type 1 Env Mutants. AIDS Res. Hum. Retroviruses. 2009 ; 25: 811-817
25. López-Balderas N., Huerta L., Villarreal C., Rivera-Toledo E., Sandoval G., Larralde C., Lamoyi E.. In Vitro Cell Fusion between CD4(+) and HIV-1 Env(+) T Cells Generates a Diversity of Syncytia Varying in Total Number, Size and Cellular Content. Virus Res. 2007 ; 123: 138-146
26. Huerta L., Lamoyi E., Baez-Saldana A., Larralde C.. Human Immunodeficiency Virus Envelope-Dependent Cell-Cell Fusion: A Quantitative Fluorescence Cytometric Assay. Cytometry. 2002 ; 47: 100-106
27. Garg H., Blumenthal R.. HIV gp41-Induced Apoptosis Is Mediated by Caspase-3-Dependent Mitochondrial Depolarization, Which Is Inhibited by HIV Protease Inhibitor Nelfinavir. J. Leukoc. Biol. 2006 ; 79: 351-362
28. Etemad-Moghadam B., Rhone D., Steenbeke T., Sun Y., Manola J., Gelman R., Fanton J. W., Racz P., Tenner-Racz K., Axthelm M. K., et al. Membrane-Fusing Capacity of the Human Immunodeficiency Virus Envelope Proteins Determines the Efficiency of CD+ T-Cell Depletion in Macaques Infected by a Simian-Human Immunodeficiency Virus. J. Virol. 2001 ; 75: 5646-5655
29. LaBonte J. A., Patel T., Hofmann W., Sodroski J.. Importance of Membrane Fusion Mediated by Human Immunodeficiency Virus Envelope Glycoproteins for Lysis of Primary CD4-Positive T Cells. J. Virol. 2000 ; 74: 10690-10698
30. +, CXCR4+ Phenotype. Immunol. Lett. 1999 ; 70: 1-3
31. Blanco J., Barretina J., Henson G., Bridger G., De Clercq E., Clotet B., Esté J. A.. The CXCR4 Antagonist AMD3100 Efficiently Inhibits Cell-Surface-Expressed Human Immunodeficiency Virus Type 1 Envelope-Induced Apoptosis. Antimicrob. Agents Chemother. 2000 ; 44: 51-56
32. Kasson P. M., Pande V. S.. Control of Membrane Fusion Mechanism by Lipid Composition: Predictions from Ensemble Molecular Dynamics. PLoS Comput. Biol. 2007 ; 3: e220
33. Rawat S. S., Viard M., Gallo S. A., Rein A., Blumenthal R., Puri A.. Modulation of Entry of Enveloped Viruses by Cholesterol and Sphingolipids [Review]. Mol. Membr. Biol. 2003 ; 20: 243-254
34. Chernomordik L. V., Kozlov M.. M: Protein-Lipid Interplay in Fusion and Fission of Biological Membranes. Annu. Rev. Biochem. 2003 ; 72: 175-207
35. Steffens C. M., Hope T. J.. Mobility of the Human Immunodeficiency Virus (HIV) Receptor CD4 and Coreceptor CCR5 in Living Cells: Implications for HIV Fusion and Entry Events. J. Virol. 2004 ; 78: 9573-9578