Detection of testosterone and epitestosterone in human hair using liquid chromatography-tandem mass spectrometry

Journal of Pharmaceutical and Biomedical Analysis

Volumn 67-68, Issue , 2012, Pages 154-158

  Deshmukh, Nawed I K ^a   Barker, James ^a   Petroczi, Andrea ^a   Naughton, Declan P ^a  

^a KINGSTON UNIVERSITY   (United Kingdom)

Author keywords

Doping; Epitestosterone; Human hair; Liquid chromatography tandem mass spectrometry; Testosterone

Indexed keywords

EPITESTOSTERONE; PENTANE; TESTOSTERONE;

ACCURACY; ARTICLE; ASSAY; FEMALE; HAIR; HAIR LEVEL; HUMAN; HUMAN EXPERIMENT; LIMIT OF DETECTION; LIMIT OF QUANTITATION; LINEAR SYSTEM; LIQUID CHROMATOGRAPHY; MALE; NORMAL HUMAN; PRIORITY JOURNAL; TANDEM MASS SPECTROMETRY;

CHROMATOGRAPHY, LIQUID; EPITESTOSTERONE; FEMALE; HAIR; HUMANS; MALE; REFERENCE STANDARDS; TANDEM MASS SPECTROMETRY; TESTOSTERONE;

EID: 84862126716     PISSN: 07317085     EISSN: 1873264X     Source Type: Journal    
DOI: 10.1016/j.jpba.2012.04.011     Document Type: Article

References (28)

1. Kintz P., Cirimele V., Sachs H., Jeanneau T., Ludes B. Testing for anabolic steroids in hair from two bodybuilders. Forensic Sci. Int. 1999, 101:209-216.
2. Deshmukh N., Hussain I., Barker J., Petroczi A., Naughton D.P. Analysis of anabolic steroids in human hair using LC-MS/MS. Steroids 2010, 75:710-714.
3. Bahrke M.S., Yesalis C.E. Abuse of anabolic androgenic steroids and related substances in sport and exercise. Curr. Opin. Pharmacol. 2004, 4:614-620.
4. Basaria S. Androgen abuse in athletes: detection and consequences. J. Clin. Endocrinol. Metab. 2010, 95:1533-1543.
5. World Anti-Doping Agency (WADA), the 2011 prohibited list - international standard. (accessed 05.01.12). http://www.wada-ama.org/Documents/World_Anti-Doping_Program/WADP-Prohibited-list/To_be_effective/WADA_Prohibited_List_2011_EN.pdf.
6. World Anti-Doping Agency (WADA), adverse analytical findings and atypical findings reported by accredited laboratories. (accessed 05.01.12). http://www.wada-ama.org/Documents/Science_Medicine/Anti-Doping_Laboratories/Lab_Statistics/WADA_2009_LaboratoryStatisticsReport_Final.pdf.
7. Sjöqvist F., Garle M., Rane A. Use of doping agents, particularly anabolic steroids, in sports and society. Lancet 2008, 371:1872-1882.
8. Fitch K.D. Androgenic-anabolic steroids and the Olympic games. Asian J. Androl. 2008, 10:384-390.
9. World Anti-Doping Agency (WADA), technical document - TD2004EAAS. (accessed 05.01.12). http://www.wada-ama.org/rtecontent/document/end_steroids_aug_04.pdf.
10. Schulze J.J., Lundmark J., Garle M., Ekström L., Sottas P.E., Rane A. Substantial advantage of a combined Bayesian and genotyping approach in testosterone doping tests. Steroids 2009, 74:365-368.
11. He C., Li S., Liu H., Li K., Liu F. Extraction of testosterone and epitestosterone in human urine using aqueous two-phase systems of ionic liquid and salt. J. Chromatogr. A 2005, 1082:143-149.
12. Sten T., Bichlmaier I., Kuuranne T., Leinonen A., Yli-Kauhaluoma J., Finel M. UDP-glucuronosyltransferases (UGTs) 2B7 and UGT2B17 display converse specificity in testosterone and epitestosterone glucuronidation, whereas UGT2A1 conjugates both androgens similarly. Drug Metab. Dispos. 2009, 37:417-423.
13. Aguilera R., Hatton C.K., Catlin D.H. Detection of epitestosterone doping by isotope ratio mass spectrometry. Clin. Chem. 2002, 48:629-636.
14. Schulze J.J., Lundmark J., Garle M., Skilving I., Ekström L., Rane A. Doping test results dependent on genotype of uridine diphospho-glucuronosyl transferase 2B17, the major enzyme for testosterone glucuronidation. J. Clin. Endocrinol. Metab. 2008, 93:2500-2506.
15. Deshmukh N., Petróczi A., Barker J., Székely A.D., Hussain I., Naughton D.P. Potentially harmful advantage to athletes: a putative connection between UGT2B17 gene deletion polymorphism and renal disorders with prolonged use of anabolic androgenic steroids. Subst. Abuse Treat. Prev. Policy 2010, 5:7. 10.1186/1747-597X-5-7.
16. Strahm E., Sottas P.E., Schweizer C., Saugy M., Dvorak J., Saudan C. Steroid profiles of professional soccer players: an international comparative study. Br. J. Sports Med. 2009, 43:1126-1130.
17. Bowers L.D. Testosterone doping: dealing with genetic differences in metabolism and excretion. J. Clin. Endocrinol. Metab. 2008, 93:2469-2471.
18. Gaillard Y., Pepin G. Testing hair for pharmaceuticals. J. Chromatogr. B 1999, 733:231-246.
19. Kintz P. Value of hair analysis in postmortem toxicology. Forensic Sci. Int. 2004, 142:127-134.
20. Min S., Ping X., Baohua S., Jun B., Mengye W. Physiological concentrations of anabolic steroids in human hair. Forensic Sci. Int. 2009, 184:32-36.
21. Deshmukh N., Hussain I., Barker J., Petroczi A., Naughton D.P. Detection of testosterone in human hair using liquid chromatography-tandem mass spectrometry. J. Pharm. Pharmacol. 2010, 62:1205-1206.
22. Scherer C., Wachter U., Wudy S.A. Determination of testosterone in human hair by gas chromatography-selected ion monitoring mass spectrometry. Analyst 1998, 123:2661-2663.
23. Wheeler M.J., Zhong Y.B., Kicman A.T., Coutts S.B. The measurement of testosterone in hair. J. Endocrinol. 1998, 159:5-8.
24. Petroczi A., Nepusz T., Cross P., Taft H., Shah S., Deshmukh N., Schaffer J., Shane M., Adesanwo C., Barker J., Naughton D.P. New non-randomised model to assess the prevalence of discriminating behaviour: a pilot study on mephedrone. Subst. Abuse Treat. Prev. Policy 2011, 6:20. 10.1186/1747-597X-6-20.
25. Petróczi A., Aidman E.V., Hussain I., Deshmukh N., Nepusz T., Uvacsek M., Tóth M., Barker J., Naughton D.P. Virtue or pretense? Looking behind self-declared innocence in doping. PLoS One 2010, 5:e10457. 10.1371/journal.pone.0010457.
26. S.A.B. Shah, N. Deshmukh, J. Barker, A. Petróczi, P. Cross, R. Archer, D.P. Naughton, Quantitative analysis of mephedrone using liquid chromatography tandem mass spectroscopy: application to human hair, J. Pharm. Biomed. Anal., doi:10.1016/j.jpba.2011.11.026.
27. Guidance for Industry Bioanalytical Method Validation. 2001 (accessed 16.03.12). http://www.fda.gov/downloads/Drugs/GuidanceComplianceRegulatoryInformation/Guidances/ucm070107.pdf.
28. Agilent, low-volume columns operational guidelines. (accessed 03.01.12). http://www.chem.agilent.com/Library/packageinsert/Public/820650-001e.pdf.