Rescue of S/MAR-containing nonviral episomal expression vectors

Cold Spring Harbor Protocols

Volumn 7, Issue 6, 2012, Pages 709-711

  Hagedorn, Claudia ^a   Baiker, Armin ^b   Postberg, Jan ^c   Ehrhardt, Anja ^a   Lipps, Hans J ^a  

^a UNIVERSITY OF WITTEN HERDECKE   (Germany)

^b BAYERISCHES LANDESAMT FÜR GESUNDHEIT UND LEBENSMITTELSICHERHEIT   (Germany)

^c Helios Medical Centre Wuppertal   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; PLASMID DNA; VIRUS VECTOR;

ARTICLE; BACTERIUM COLONY; BACTERIUM TRANSFORMATION; CELL CLONE; DNA ISOLATION; EPISOME; ESCHERICHIA COLI; EXPRESSION VECTOR; MATRIX ATTACHMENT REGION; NONHUMAN; GENE VECTOR; GENETIC ENGINEERING; GENETIC TRANSFORMATION; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; PLASMID;

ESCHERICHIA COLI; GENETIC ENGINEERING; GENETIC VECTORS; PLASMIDS; TRANSFORMATION, BACTERIAL; TRANSFORMATION, GENETIC;

EID: 84862123808     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot069518     Document Type: Article

References (4)

1. Hagedorn C, Baiker A, Postberg J, Ehrhardt A, Lipps HJ. 2012a. Handling S/MAR Vectors. Cold Spring Harb Protoc. doi: 10.1101/pdb.top068262.
2. Hagedorn C, Baiker A, Postberg J, Ehrhardt A, Lipps HJ. 2012b. A colonyforming assay for determining the establishment efficiency of S/MAR- containing nonviral episomal expression vectors. Cold Spring Harb Protoc. doi: 10.1101/pdb.prot069500.
3. Hirt B. 1967. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol 26: 365-369.
4. Sambrook J, Russell DW. 2001. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.