Establishment and optimization of two dimensional electrophoresis system for analyzing protein profiles in stylo roots

Zhiwu Shengli Xuebao/Plant Physiology Journal

Volumn 47, Issue 2, 2011, Pages 199-204

  Chen, Zhi Jian ^a   Yan, Wei ^a,b   Sun, Li Li ^a,b   Liu, Guo Dao ^b   Liao, Hong ^a   Tian, Jiang ^a  

^a SOUTH CHINA AGRICULTURAL UNIVERSITY   (China)

^b INSTITUTE OF TROPICAL BIOSCIENCE AND BIOTECHNOLOGY   (China)

Author keywords

2 DE; Protein extraction; Stylo

Indexed keywords

STYLOSANTHES GUIANENSIS;

EID: 84862087408     PISSN: 20951108     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. 2002. 22 (2): 11-23
2. 2006. 23 (9): 41-44
3. Bona E, Marsano F, Cavaletto M, Berta G (2007). Proteomic characterization of copper stress response in Cannabis sativa roots. Proteomics, 7: 1121-1130
4. Carpentier S C, Witters E, Laukens K, Deckers P, Swennen R, Panis B (2005). Preparation of protein extracts from recalcitrant plant tissues: an evaluation of different methods for two dimensional gel electrophoresis analysis. Proteomics, 5: 2497-2507
5. Chang S, Puryear J, Cairney J (1993). A simple efficient method for isolating RNA from pine trees. Plant Mol Biol Rep, 11: 113-116
6. Chen S, Harmon A C (2006). Advances in plant proteomics. Proteomics, 6: 5504-5516
7. Cheng Y J, Guo W W, Yi H L, Pang X M, Deng X X (2003). An efficient protocol for genomic DNA extraction from Citrus species. Plant Mol Biol Rep, 21:177a-177g
8. Fang G, Hammar S, Grumet R (1992). A quick and inexpensive method for removing polysaccharides from plant genomic DNA. Biotechniques, 13: 52-54, 56
9. Giavalisco P, Nordhoff E, Lehrach H, Gobom J, Klose J (2003). Extraction of proteins from plant tissues for two-dimensional electrophoresis analysis. Electrophoresis, 24: 207-216
10. Gorg A, Weiss W, Dunn M J (2004). Current two dimensional electrophoresis technology for proteomics. Proteomics, 4: 3665-3685
11. Hille J M, Freed A L, Watzig H (2001). Possibilities to improve automation, speed and precision of proteome analysis: a comparison of two-dimensional elect rophoresis and alternatives. Electrophoresis, 22: 4035-4052
12. Hurkman W J, Tanaka C K (1986). Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis. Plant Physiol, 81: 802-806
13. Lewinsohn E, Steele C L, Croteau R (1994). Simple isolation of functional RNA from woody stems of gymnosperms. Plant Mol Biol Rep, 12: 20-25
14. Li K, Xu C, Zhang K, Yang A, Zhang J (2007). Proteomic analysis of roots growth and metabolic changes under phosphorus deficit in maize (Zea mays L.) plants. Proteomics, 7: 1501-1512
15. Liao H, Wan H Y, Shaff J, Wang X R, Yan X L, Kochian L V (2006). Phosphorus and aluminum interactions in soybean in relation to Al tolerance: exudation of specific organic acids from different regions of the intact root system. Plant Physiol, 72: 19-23
16. Liu G D, Phaikaew C, Stur W W (1997). Status of Stylosanthes development in other countries. II. Stylosanthes development and utilization in China and south-east Asia. Tropic Grasslands, 31: 460-466
17. Nagai K, Yotsukura N, Lkegami H, Kimura H, Morimoto K (2008). Protein extraction for 2-DE from the lamina of Ecklonia kurome (laminariales): recalcitrant tissue containing high levels of viscous polysaccharides. Electrophoresis, 29: 672-681
18. Patterson S D, Aebersold R H (2003). Proteomics: the first decade and beyond. Nat Genet, 33: 311-323
19. Rabilloud T (1998). Use of thiourea to increase the solubility of membrane proteins in two-dimensional electrophoresis. Electrophoresis, 19: 758-760
20. Salekdeh G H, Siopongco J, Wade L J, Ghareyazie B, Bennett J (2002). A proteomic approach to analyzing drought- and salt-responsiveness in rice. Field Crops Res, 76: 199-219
21. Sauer M, Jakob A, Nordheim A, Hochholdinger F (2006). Proteomic analysis of shoot borne root initiation in maize (Zea mays L.). Proteomics, 6: 2530-2541
22. Shaw M M, Riederer B M (2003). Sample preparation for two dimensional gel electrophoresis. Proteomics, 3: 1408-1417
23. Tesniere C, Vayda M E (1991). Method for the isolation of highquality RNA from grape berry tissues without contaminating tannins or carbohydrates. Plant Mol Biol Rep, 9: 242-251
24. Tsugita A, Kamo M (1999). 2-D Electrophoresis of plant proteins. Methods Mol Biol, 112: 95-97
25. Witzel K, Surabhi G K, Jyothsnakumari G, Sudhakar C, Matros A, Mock P (2007). Quantitative proteome analysis of barley seeds using ruthenium (II)-tris-(bathophenanthrolinedisulphonate) staining. J Proteome Res, 6: 1325-1333
26. Xie H, Pan S, Liu S, Ye K, Huo K (2007). A novel method of protein extraction from perennial Bupleurum root for 2-DE. Electrophoresis, 28: 871-875
27. Xu C, Sullivan J H, Garrett W M, Caperna T J, Natarajan S (2008). Impact of solar ultraviolet-B on the proteome in soybean lines differing in flavonoid contents. Phytochemistry, 69: 38-48
28. Yan S P, Zhang Q Y, Tang Z C, Su W A, Sun W N (2006). Comparative proteomic analysis provides new insights into chilling stress responses in rice. Mol Cell Proteomics, 5: 484-496
29. Yin L, Tao Y, Zhao K, Shao J, Li X, Liu G, Liu S, Zhu L (2007). Proteomic and transcriptomic analysis of rice mature seed derived callus differentiation. Proteomics, 7: 755-768