Performance of the roche lightcycler real-time PCR assay for diagnosing extrapulmonary tuberculosis

Journal of Clinical Microbiology

Volumn 50, Issue 6, 2012, Pages 2100-2103

  Gous, Natasha ^a   Scott, L E ^a   Wong, E ^b   Omar, T ^c   Venter, W D F ^b   Stevens, W ^a,d  

^a UNIVERSITY OF THE WITWATERSRAND   (South Africa)

^b UNIVERSITY OF THE WITWATERSRAND   (South Africa)

^c UNIVERSITY OF THE WITWATERSRAND   (South Africa)

^d NATIONAL HEALTH LABORATORY SERVICE   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BONE MARROW BIOPSY; CEREBROSPINAL FLUID; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC KIT; DIAGNOSTIC TEST ACCURACY STUDY; EXTRAPULMONARY TUBERCULOSIS; HUMAN; HUMAN TISSUE; INTERMETHOD COMPARISON; LYMPH NODE BIOPSY; MYCOBACTERIUM AVIUM; MYCOBACTERIUM KANSASII; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; SAFETY; SENSITIVITY AND SPECIFICITY;

ADULT; AGED; AGED, 80 AND OVER; BACTERIOLOGICAL TECHNIQUES; BONE MARROW; CEREBROSPINAL FLUID; FEMALE; HUMANS; LIVER; LUNG; LYMPH NODES; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; MYCOBACTERIUM TUBERCULOSIS; REAL-TIME POLYMERASE CHAIN REACTION; TUBERCULOSIS;

MYCOBACTERIUM; MYCOBACTERIUM AVIUM; MYCOBACTERIUM KANSASII; MYCOBACTERIUM TUBERCULOSIS;

EID: 84862080206     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00252-12     Document Type: Article

References (36)

1. Ansari NA, et al. 2002. Pathology and causes of death in a group of 128 predominantly HIV-positive patients in Botswana, 1997-1998. Int. J. Tuberc. Lung Dis. 6:55- 63.
2. Armand S, Vanhuls P, Delcroix G, Courcol R, Lemaitre N. 2011. Comparison of the Xpert MTB/RIF test with an IS6110-TaqMan real-time PCR assay for direct detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens. J. Clin. Microbiol. 49:1772-1776.
3. Azov AG, Koch J, Hamilton-Dutoit SJ. 2005. Improved diagnosis of mycobacterial infections in formalin-fixed and paraffin-embedded sections with nested polymerase chain reaction. APMIS 113:586-593. (Pubitemid 41577154)
4. Bowles EC, et al. 2011. Xpert MTB/RIF(R), a novel automated polymerase chain reaction-based tool for the diagnosis of tuberculosis. Int. J. Tuberc. Lung Dis. 15:988-989.
5. Brown G. 1978. An introduction to histotechnology: a manual for the student, practicing technologist, and resident-in-pathology. Appleton-Century-Crofts, New York, NY.
6. Chawla K, Gupta S, Mukhopadhyay C, Rao PS, Bhat SS. 2009. PCR for M. tuberculosis in tissue samples. J. Infect. Dev. Ctries. 3:83-87.
7. .Chosamata B, Scott L, Mahlangu J. 2010. Abstr. 23rd Int. Symp. Technol. Innov. Lab. Haematol., abstr 107, p 75. International Society for Laboratory Haematology, Brighton, United Kingdom.
8. de Wit D, Maartens G, Steyn L. 1992. A comparative study of the polymerase chain reaction and conventional procedures for the diagnosis of tuberculous pleural effusion. Tuber. Lung Dis. 73:262-267.
9. Guillerm M, Usdin M, Arkinstall J. 2006. Tuberculosis diagnosis and drug sensitivity testing: an overview of the current diagnostic pipeline. Doctors Without Borders, Geneva, Switzerland.
10. Hillemann D, Galle J, Vollmer E, Richter E. 2006. Real-time PCR assay for improved detection of Mycobacterium tuberculosis complex in paraffin-embedded tissues. Int. J. Tuberc. Lung Dis. 10:340-342.
11. Hillemann D, Ruesch-Gerdes S, Boehme C, Richter E. 2011. Rapid molecular detection of extrapulmonary tuberculosis by automated Gene-Xpert(R) MTB/RIF system. J. Clin. Microbiol. 49:1202-1205.
12. Ligthelm LJ, et al. 2011. Xpert MTB/RIF for rapid diagnosis of tuberculous lymphadenitis from fine-needle-aspiration biopsy specimens. J. Clin. Microbiol. 49:3967-3970.
13. Lucas SB, et al. 1993. The mortality and pathology of HIV infection in a West African city. AIDS 7:1569 -1579.
14. Malbruny B, Le Marrec G, Courageux K, Leclercq R, Cattoir V. 2011. Rapid and efficient detection of Mycobacterium tuberculosis in respiratory and non-respiratory samples. Int. J. Tuberc. Lung Dis. 15:553-555.
15. Mishra PK, et al. 2010. Molecular detection of Mycobacterium tuberculosis in formalin-fixed, paraffin-embedded tissues and biopsies of gastro-intestinal specimens using real-time polymerase chain reaction system. Turk. J. Gastroenterol. 21:129 -134.
16. Monkongdee P, et al. 2009. Yield of acid-fast smear and mycobacterial culture for tuberculosis diagnosis in people with human immunodeficiency virus. Am. J. Respir. Crit. Care Med. 180:903-908.
17. Moure R, et al. 2011. Rapid detection of Mycobacterium tuberculosis complex and rifampin resistance in smear-negative clinical samples by use of an integrated real-time PCR method. J. Clin. Microbiol. 49: 1137-1139.
18. Park JS, et al. 2010. Nested PCR in lung tissue for diagnosis of pulmonary tuberculosis. Eur. Respir. J. 35:851-857.
19. Perosio PM, Frank TS. 1993. Detection and species identification of mycobacteria in paraffin sections of lung biopsy specimens by the polymerase chain reaction. Am. J. Clin. Pathol. 100:643-647. (Pubitemid 24004120)
20. Polesky A, Grove W, Bhatia G. 2005. Peripheral tuberculous lymphadenitis: epidemiology, diagnosis, treatment, and outcome. Medicine (Baltimore) 84:350 -362.
21. Prophet EB, Mills B, Arrington J, Sobin L. 1992. Laboratory methods in histotechnology. Armed Forces Institute of Pathology, Washington, DC.
22. Rana FS, et al. 2000. Autopsy study of HIV-1-positive and HIV-1-negative adult medical patients in Nairobi, Kenya. J. Acquir. Immune Defic. Syndr. 24:23-29. (Pubitemid 30447802)
23. Ritis K, et al. 2005. Diagnostic usefulness of bone marrow aspiration material for the amplification of IS6110 insertion element in extrapulmonary tuberculosis: comparison of two PCR techniques. Int. J. Tuberc. Lung Dis. 9:455- 460.
24. Ritis K, et al. 2000. Amplification of IS6110 sequence for detection of Mycobacterium tuberculosis complex in HIV-negative patients with fever of unknown origin (FUO) and evidence of extrapulmonary disease. J. Intern. Med. 248:415- 424.
25. Roche Diagnostics GmbH. 2009. LightCycler mycobacterium detection kit manual. Roche Diagnostics GmbH, Mannheim, Germany.
26. Roche Diagnostics GmbH. 2008. MagNA Pure LC DNA isolation kit III (bacteria, fungi) manual. Roche Diagnostics GmbH, Mannheim, Germany.
27. Salian NV, Rish JA, Eisenach KD, Cave MD, Bates JH. 1998. Polymerase chain reaction to detect Mycobacterium tuberculosis in histologic specimens. Am. J. Respir. Crit. Care Med. 158:1150-1155. (Pubitemid 28489015)
28. Scott LE, et al. 2011. Comparison of Xpert MTB/RIF with other nucleic acid technologies for diagnosing pulmonary tuberculosis in a high HIV prevalence setting: a prospective study. PLoS Med. 8:e1001061. doi: 10.1371/journal.pmed. 1001061.
29. Sharma SK, Mohan A. 2004. Extrapulmonary tuberculosis. Indian J. Med. Res. 120:316-353. (Pubitemid 39504471)
30. Shrivastava R, Punde RP, Pandey H, Samarth RM, Maudar KK. 2010. Evolutionary tools in the molecular diagnosis of Mycobacterium tuberculosis: a review. J. Med. Sci. 10:124 -129.
31. Soini H, Musser JM. 2001. Molecular diagnosis of mycobacteria. Clin. Chem. 47:809-814. (Pubitemid 32398030)
32. Vadwai V, et al. 2011. Xpert MTB/RIF: a new pillar in diagnosis of extrapulmonary tuberculosis? J. Clin. Microbiol. 49:2540 -2545.
33. Wilson D, Nachega JB, Chaisson RE, Maartens G. 2005. Diagnostic yield of peripheral lymph node needle-core biopsies in HIV-infected adults with suspected smear-negative tuberculosis. Int. J. Tuberc. Lung Dis. 9:220 -222.
34. World Health Organization. 2009. Tuberculosis country profile: South Africa 2009. World Health Organization, Geneva, Switzerland.
35. World Health Organization. 2007. Improving the diagnosis and treatment of smear-negative pulmonary and extrapulmonary tuberculosis among adults and adolescents: Recommendations for HIV-prevalent and resource-constrained settings. Stop TB Department and Department of HIV/AIDS, World Health Organization, Geneva, Switzerland.
36. Zwang J, Garenne M, Kahn K, Collinson M, Tollman S. 2007. Trends in mortality from pulmonary tuberculosis and HIV/AIDS co-infection in rural South Africa (Agincourt). Trans. R. Soc. Trop. Med. Hyg. 101:893-898. (Pubitemid 47091461)