Detection of dengue virus genome in urine by real-time reverse transcriptase PCR: A laboratory diagnostic method useful after disappearance of the genome in serum

Journal of Clinical Microbiology

Volumn 50, Issue 6, 2012, Pages 2047-2052

  Hirayama, Takanori ^a,b,d   Mizuno, Yasutaka ^c,e   Takeshita, Nozomi ^c   Kotaki, Akira ^a   Tajima, Shigeru ^a   Omatsu, Tsutomu ^a   Sano, Kouichi ^d   Kurane, Ichiro ^a   Takasaki, Tomohiko ^a  

^a NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

^b Japan Advanced Institute of Science and Technology   (Japan)

^c NATIONAL CENTER FOR GLOBAL HEALTH AND MEDICINE   (Japan)

^d OSAKA MEDICAL COLLEGE   (Japan)

^e TOKYO MEDICAL UNIVERSITY HOSPITAL   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN M ANTIBODY;

ADOLESCENT; ADULT; AGED; ARTICLE; BLOOD ANALYSIS; CHILD; CONTROLLED STUDY; DENGUE; DENGUE VIRUS; DISEASE COURSE; DISEASE DURATION; GENETIC IDENTIFICATION; HUMAN; INTERMETHOD COMPARISON; LABORATORY DIAGNOSIS; MAJOR CLINICAL STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCHOOL CHILD; SERUM; URINALYSIS; VIREMIA; VIRUS GENOME; VIRUS ISOLATION;

ADOLESCENT; ADULT; AGED; CHILD; DENGUE; DENGUE VIRUS; FEMALE; HUMANS; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SERUM; TIME FACTORS; URINE; VIROLOGY; YOUNG ADULT;

DENGUE VIRUS;

EID: 84862068642     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.06557-11     Document Type: Article

References (16)

1. Amarilla AA, et al. 2009. Genetic diversity of the E protein of dengue type 3 virus. Virol. J. 6:113.
2. Basílio-de-Oliveira CA, Aquiar GR, Baldanza MS, Birth OM, Eyer Silva WA, Paes MV. 2005. Pathologic study of fatal case of dengue-3 virus infection in Rio de Janeiro, Brazil. Braz. J. Infect. Dis. 9:341-347. (Pubitemid 43141340)
3. Callahan JD, et al. 2001. Development and evaluation of serotype- and group-specific fluorogenic reverse transcriptase PCR (TaqMan) assays for dengue virus. J. Clin. Microbiol. 39:4119-4124. (Pubitemid 33029792)
4. Halstead SB. 2007. Dengue. Lancet 370:1644-1652. (Pubitemid 350052290)
5. Ito M, et al. 2004. Development and evaluation of fluorogenic TaqMan reverse transcriptase PCR assays for detection of dengue virus type 1 to 4. J. Clin. Microbiol. 42:5935-5937. (Pubitemid 39664400)
6. Itoda I, et al. 2006. Clinical features of 62 imported cases of dengue fever in Japan. Am. J. Trop. Med. Hyg. 75:470-474. (Pubitemid 46941307)
7. Jessie K, Fong MY, Devi S, Law SK, Wong KT. 2004. Localization of dengue virus in naturally infected human tissues, by immunohistochemistry and in situ hybridization. J. Infect. Dis. 189:1411-1418. (Pubitemid 38543590)
8. Johnson BW, Russell BJ, Lanciotti RS. 2005. Serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase PCR assay. J. Clin. Microbiol. 10:4977-4983. (Pubitemid 41483993)
9. Karen LR, Kenneth LT. 2008. Viral encephalitis, p 2629-2633. In Fauci A et al. (ed), Harrison's principles of internal medicine, 17th ed. McGraw- Hill, New York, NY.
10. Kumarasamy V et al. 2007. Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection. J. Virol. Methods 140:75-79. (Pubitemid 46201707)
11. Mizuno Y, et al. 2007. Confirmation of dengue virus infection by detection of dengue virus type 1 genome in urine and saliva but not in plasma. Trans. R. Soc. Trop. Med. Hyg. 101:738-739. (Pubitemid 46706148)
12. Poloni TR, et al. 2010. Detection of dengue virus in saliva and urine by real time RT-PCR. Virol. J. 7:22.
13. Tonry JH, et al. 2005. West Nile virus detection in urine. Emerg. Infect. Dis. 11:1294-1296. (Pubitemid 41045342)
14. Vaughn DW, et al. 1997. Dengue in the early febrile phase: viremia and antibody responses. J. Infect. Dis. 176:322-330. (Pubitemid 27328753)
15. Vázquez S, et al. 2007. Kinetics of antibodies in sera, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infections. Int. J. Infect. Dis. 11:256-262. (Pubitemid 46629037)
16. World Health Organization. 2009. Dengue: guidelines for diagnosis, treatment, prevention and control. WHO, Geneva, Switzerland.