Large, but not small sustained tensile strains stimulate adipogenesis in culture

Annals of Biomedical Engineering

Volumn 40, Issue 5, 2012, Pages 1052-1060

  Levy, Ayelet ^a   Enzer, Sarit ^a   Shoham, Naama ^a   Zaretsky, Uri ^a   Gefen, Amit ^a  

^a TEL AVIV UNIVERSITY   (Israel)

Author keywords

3T3 L1 preadipocytes; Differentiation; Lipid droplets; Mechanotrasduction; Strecth deformation

Indexed keywords

ADIPOCYTES; ADIPOGENESIS; ADIPOGENIC; CONTROL CULTURES; EXPERIMENTAL DATA; LIPID DROPLETS; LONG-TERM EFFECTS; MECHANICAL STIMULUS; MECHANOTRASDUCTION; MEDICAL CONDITIONS; MITOGEN-ACTIVATED PROTEIN KINASE KINASE; NON DESTRUCTIVE; PHYSIOLOGICAL RANGE; PREADIPOCYTES; SIGNALING PATHWAYS; STRAIN THRESHOLD;

DIFFERENTIATION (CALCULUS); DROP BREAKUP; ENZYMES; PHYSIOLOGY;

TENSILE STRAIN;

ADIPOGENESIS; ANIMAL; ARTICLE; CELL STRAIN 3T3; CULTURE TECHNIQUE; METHODOLOGY; MOUSE; PHYSIOLOGICAL STRESS; PHYSIOLOGY; TIME;

3T3-L1 CELLS; ADIPOGENESIS; ANIMALS; CELL CULTURE TECHNIQUES; MICE; STRESS, PHYSIOLOGICAL; TIME FACTORS;

EID: 84862017920     PISSN: 00906964     EISSN: 15739686     Source Type: Journal    
DOI: 10.1007/s10439-011-0496-x     Document Type: Article

References (35)

1. Arathuzik, G. G., and A. E. Goebel-Fabbri. Nutrition therapy and the management of obesity and diabetes: an update. Curr. Diab. Rep. 11:106-110, 2011.
2. Arner, P., and K. L. Spalding. Fat cell turnover in humans. Biochem. Biophys. Res. Commun. 396:101-104, 2010.
3. Azagury, D. E., and D. B. Lautz. Obesity overview: epidemiology, health and financial impact, and guidelines for qualification for surgical therapy. Gastrointest. Endosc. Clin. N. Am. 21:189-201, 2011.
4. Burger, E. H., and J. Klein-Nulend. Mechanotransduction in bone-role of the lacunocanalicular network. FASEB J. 13:S101-S112, 1999.
5. Crossno, J. T., S. M. Majka, T. Grazia, R. G. Gill, and D. J. Klemm. Rosiglitazone promotes development of a novel adipocyte population from bone marrow-derived circulating progenitor cells. J. Clin. Invest. 116:3220-3228, 2006. (Pubitemid 44878636)
6. Furukawa, N., P. Ongusaha, W. J. Jahng, K. Araki, C. S. Choi, H. J. Kim, Y. H. Lee, K. Kaibuchi, B. B. Kahn, H. Masuzaki, J. K. Kim, S. W. Lee, and Y. B. Kim. Role of Rho-kinase in regulation of insulin action and glucose homeostasis. Cell Metab. 2:119-129, 2005.
7. Geddes, D. M., and R. S. Cardill, II. An in vitro model of neural trauma: device characterization and calcium response to mechanical stretch. J. Biomech. Eng. 123:247-255, 2001. (Pubitemid 32643202)
8. Gefen, A., and M. Be'ery-Lipperman. Contribution of muscular weakness on osteoporosis: computational and animal models. Clin. Biomech. 20:984-997, 2005. (Pubitemid 41376517)
9. Grandolfo, M., A. Calabrese, and P. D'Andrea. Mechanism of mechanically induced intercellular calcium waves in rabbit articular chondrocytes and in HIG-82 synovial cells. J. Bone Miner. Res. 13:443-453, 1998. (Pubitemid 28146227)
10. Gregoire, F. M., C. M. Smas, and H. S. Sook. Understanding adipocytes differentiation. Physiol. Rev. 78(3): 783-809, 1998.
11. Hara, Y., S. Wakino, Y. Tanabe, M. Saito, H. Tokuyama, N. Washida, S. Tatematsu, K. Yoshioka, K. Homma, K. Hasegawa, H. Minakuchi, K. Fujimura, K. Hosoya, K. Hayashi, K. Nakayama, and H. Itoh. Rho and Rho-kinase activity in adipocytes contributes to a vicious cycle in obesity that may involve mechanical stretch. Sci. Signal. 4:157 ra3, 2011.
12. Hossain, M. G., T. Iwata, N. Mizusawa, S. W. Shima, T. Okutsu, K. Ishimoto, and K. Ypshimoto. Compressive force inhibits adipogenesis through COX-2-mediated down-regulation of PPARgamma2 and C/EBPalpha. J. Biosci. Bioeng. 109:297-303, 2010.
13. Huang, S. C., T. C. Wu, H. C. Yu, M. R. Chen, C. M. Liu, and W. S. Chiang. Mechanical strain modulates age-related changes in the proliferation and differentiation of mouse adipose-derived stromal cells. BMC Cell Biol. 10:11-18, 2010.
14. Jaalouk, D. E., and J. Lammerding. Mechanotransduction gone awry. Nat. Rev. Mol. Cell Biol. 10:63-73, 2009.
15. Jacobs, C. R., S. Temiyasathit, and A. B. Castillo. Osteocyte mechanobiology and pericellular mechanics. Annu. Rev. Biomed. Eng. 12:369-400, 2010.
16. Kato, H., H. Suga, H. Eto, J. Araki, N. Aoi, K. Doi, T. Iida, Y. Tabata, and K. Yoshimura. Reversible adipose tissue enlargement induced by external tissue suspension: possible contribution of basic fibroblast growth factor in the preservation of enlarged tissue. Tissue Eng. Part A 16(6):2029-2040, 2010.
17. Lee, A. A., T. Delhaas, L. K. Waldman, D. A. Mack Kenna, F. J. Villarreal, and A. D. McCulloch. An equibiaxial strain system for cultured cells. Am. J. Physiol. Cell Physiol. 271:1400-1408, 1996.
18. Linder-Ganz, E., N. Shabshin, Y. Itzchak, and A. Gefen. Assessment of mechanical conditions in sub-dermal tissues during sitting: a combined experimental-MRI and finite element approach. J. Biomech. 40:1443-1454, 2007. (Pubitemid 46601441)
19. Linder-Ganz, E., N. Shabshin, Y. Itzchak, Z. Yizhar, I. Siev-Ner, and A. Gefen. Strains and stresses in sub-dermal tissues of the buttocks are greater in paraplegics than in healthy during sitting. J. Biomech. 41:567-580, 2008. (Pubitemid 351179714)
20. Or-Tzadikario, S., R. Sopher, and A. Gefen. Quantitative monitoring of lipid accumulation over time in cultured adipocytes as function of culture conditions: toward controlled adipose tissue engineering. Tissue Eng. 16:1167-1181, 2010.
21. Prusty, D., B. Park, K. E. Davis, and S. R. Farmer. Activation of MEK/ERK signaling promotes adipogenesis by enhancing peroxisome proliferator-activated receptor # (PPAR#) and C/EBPa gene expression during the differentiation of 3 T3-L1 preadipocytes. J. Biol. Chem. 277: 46226-46232, 2002. (Pubitemid 35417614)
22. Ramirez-Zacarias, J., F. Castro-Munozledo, and W. Kuri- Harcuch. Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry 97:493-497, 1992.
23. Rubin, C. T., E. Capilla, Y. K. Luu, B. Busa, H. Crawford, D. J. Nolan, V. Mittal, C. J. Rosen, J. E. Pessin, and S. Judex. Adipogenesis is inhibited by brief, daily exposure to high-frequency, extremely low-magnitude mechanical signals. Proc. Natl Acad. Sci. USA 104:17879-17884, 2010. (Pubitemid 350210928)
24. Sadoshima, J., and S. Izumo. The cellular and molecular response of cardiac myocytes to mechanical stress. Annu. Rev. Physiol. 59:551-571, 1997. (Pubitemid 27142455)
25. Shabshin, N., V. Ougortsin, G. Zoizner, and A. Gefen. Evaluation of the effect of trunk tilt on compressive soft tissue deformations under the ischial tuberosities using weight-bearing MRI. Clin. Biomech. 25:402-408, 2010.
26. Shabshin, N., G. Zoizner, A. Herman, V. Ougortsin, and A. Gefen. Use of weight-bearing MRIfor evaluating wheelchair cushions based on internal soft-tissue deformations under ischial tuberosities. J. Rehabil. Res. Dev. 47:31-42, 2010.
27. Shoham, N., and A. Gefen. Mechanotransduction in adipocytes. J. Biomech. doi:10.1016/j.jbiomech.2011.10.023.
28. Shoham, N., R. Gottlieb, O. Sharabani-Yosef, U. Zaretsky, D. Benayahu, and A. Gefen. Static mechanical stretching accelerates lipid production in 3 T3-L1 adipocytes by activating the MEK signaling pathway. Am. J. Physiol. Cell Physiol. doi:10.1152/ajpcell.00167.2011.
29. Slomka, N., and A. Gefen. Relationship between strain levels and permeability of the plasma membrane in statically stretched myoblasts. Ann. Biomed. Eng., 2011. doi: 10.1007/s10439-011-0423-1.
30. Slomka, N., S. Or-Tzadikario, D. Sassun, and A. Gefen. Membrane-stretch-induced cell death in deep tissue injury: computer model studies. Cell Mol. Bioeng. 2:118-132, 2009.
31. Sopher, R., J. Nixon, C. Gorecki, and A. Gefen. Effects of intramuscular fat infiltration, scarring, and spasticity on the risk for sitting-acquired deep tissue injury in spinal cord injury patients. J. Biomech. Eng. 133:021011, 2011.
32. Tanabe, Y., M. Koga, M. Saito, Y. Matsunaga, and K. Nakayama. Inhibition of adipocyte differentiation by mechanical stretching through ERK-mediated downregulation of PPARgamma2. J. Cell Sci. 117:3605-3614, 2004. (Pubitemid 39206400)
33. Tanabe, Y., Y. Matsunaga, M. Saito, and K. Nakayama. Involvement of cyclooxygenase-2 in synergistic effect of cyclic stretching and eicosapentaenoic acid on adipocyte differentiation. J. Pharmacol. Sci. 106:478-484, 2008. (Pubitemid 351468601)
34. Turner, N. J., H. S. Jones, J. E. Davies, and A. E. Canfield. Cyclic stretch-induced TGFb1/Smad signaling inhibits adipogenesis in umbilical cord progenitor cells. Biochem. Biophys. Res. Commun. 377:1147-1151, 2008.
35. Wang, J. H., and B. P. Thampatty. An introductory review of cell mechanobiology. Biomech. Model. Mechanobiol. 5:1-16, 2006. (Pubitemid 43261226)