Optimising the self-assembly of siRNA loaded PEG-PCL-lPEI nano-carriers employing different preparation techniques

Journal of Controlled Release

Volumn 160, Issue 3, 2012, Pages 583-591

  Endres, Thomas ^a   Zheng, Mengyao ^a   Beck Broichsitter, Moritz ^a   Samsonova, Olga ^a   Debus, Heiko ^a   Kissel, Thomas ^a  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

Cationic triblock copolymer; Complexation; Microfluidic; Self assembly; siRNA

Indexed keywords

AMPHIPHILICS; BULK MIXING; CAPROLACTONE; CATIONIC POLYMERS; CENTRAL COMPOSITE DESIGNS; CHARGE RATIO; COLLOIDAL STABILITY; CONSTANT RATIO; GENE DELIVERY; IN-VITRO; MICROFLUIDIC MIXING; MIXING PROCESS; NANO-SIZED PARTICULATES; ORGANIC PHASE; POLYETHYLENE IMINES; POLYMER DESIGNS; PREPARATION TECHNIQUE; SIRNA; SURFACE COATINGS; TRANSFECTION EFFICIENCY; UNIFORM CHARGE DISTRIBUTION; VECTOR SYSTEMS;

BLOCK COPOLYMERS; COMPLEXATION; EFFICIENCY; GENE TRANSFER; MICROFLUIDICS; MIXING; POLYETHYLENE GLYCOLS; RNA; SELF ASSEMBLY;

LOADING;

NANOCARRIER; NANOCOMPOSITE; POLYCAPROLACTONE; POLYETHYLENEIMINE; SMALL INTERFERING RNA;

AQUEOUS SOLUTION; ARTICLE; COLLOID; COMPLEX FORMATION; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; DRUG FORMULATION; GENETIC TRANSFECTION; INTERMETHOD COMPARISON; LIGHT SCATTERING; MICROFLUIDICS; MOLECULAR STABILITY; NANOPHARMACEUTICS; NONVIRAL GENE DELIVERY SYSTEM; PARTICLE SIZE; PRIORITY JOURNAL; PROCESS OPTIMIZATION; RESPONSE SURFACE METHOD; TRANSMISSION ELECTRON MICROSCOPY; ZETA POTENTIAL;

CELL LINE, TUMOR; GENE EXPRESSION; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (PHOSPHORYLATING); HEPARIN; HUMANS; IMINES; PARTICLE SIZE; POLYESTERS; POLYETHYLENE GLYCOLS; POLYETHYLENES; RIBONUCLEASES; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 84861981982     PISSN: 01683659     EISSN: 18734995     Source Type: Journal    
DOI: 10.1016/j.jconrel.2012.04.013     Document Type: Article

References (39)

1. M. Ferrari, Experimental therapies: vectoring siRNA therapeutics into the clinic, Nat. Rev. Clin. Oncol. 7 (2010) 485-486.
2. D.J. Gary, N. Puri, Y.Y. Won, Polymer-based siRNA delivery: perspectives on the fundamental and phenomenological distinctions from polymer-based DNA delivery, J. Control. Release 121 (2007) 64-73. (Pubitemid 47126065)
3. C.Q. Mao, J.Z. Du, T.M. Sun, Y.D. Yao, P.Z. Zhang, E.W. Song, J. Wang, A biodegradable amphiphilic and cationic triblock copolymer for the delivery of siRNA targeting the acid ceramidase gene for cancer therapy, Biomaterials 32 (2011) 3124-3133.
4. Y. Wang, S. Gao, W.H. Ye, H.S. Yoon, Y.Y. Yang, Co-delivery of drugs and DNA from cationic core-shell nanoparticles self-assembled from a biodegradable copolymer, Nat. Mater. 5 (2006) 791-796. (Pubitemid 44570860)
5. X. Shuai, T. Merdan, F. Unger, M. Wittmar, T. Kissel, Novel biodegradable ternary copolymers hy-PEI-g-PCL-b-PEG, Macromolecules 36 (2003) 5751-5759 (Washington, DC, United States).
6. T.K. Endres, M. Beck-Broichsitter, O. Samsonova, T. Renette, T.H. Kissel, Self-assembled biodegradable amphiphilic PEG-PCL-lPEI triblock copolymers at the borderline between micelles and nanoparticles designed for drug and gene delivery, Biomaterials 32 (2011) 7721-7731.
7. J. Jeong, T. Park, S. Kim, Self-assembled and nanostructured siRNA delivery systems, Pharm. Res. 28 (2011) 2072-2085.
8. C. Zhu, S. Jung, S. Luo, F. Meng, X. Zhu, T.G. Park, Z. Zhong, Co-delivery of siRNA and paclitaxel into cancer cells by biodegradable cationic micelles based on PDMAEMA-PCL-PDMAEMA triblock copolymers, Biomaterials 31 (2010) 2408-2416.
9. J.C. Kasper, D. Schaffert, M. Ogris, E. Wagner, W. Friess, The establishment of an up-scaled micro-mixer method allows the standardized and reproducible preparation of well-defined plasmid/LPEI polyplexes, Eur. J. Pharm. Biopharm. 77 (2011) 182-185.
10. F. Bordi, C. Cametti, M. Diociaiuti, D. Gaudino, T. Gili, S. Sennato, Complexation of anionic polyelectrolytes with cationic liposomes: evidence of reentrant condensation and lipoplex formation, Langmuir 20 (2004) 5214-5222.
11. Y. Ding, X. Bian, W. Yao, R. Li, D. Ding, Y. Hu, X. Jiang, Y. Hu, Surface-potential-regulated transmembrane and cytotoxicity of chitosan/gold hybrid nanospheres, ACS Appl. Mater. Interfaces 2 (2010) 1456-1465.
12. P.R. Leroueil, S. Hong, A. Mecke, J.R. Baker, B.G. Orr, M.M.B. Holl, Nanoparticle interaction with biological membranes: does nanotechnology present a Janus face? Acc. Chem. Res. 40 (2007) 335-342.
13. D. Fischer, Y. Li, B. Ahlemeyer, J. Krieglstein, T. Kissel, In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis, Biomaterials 24 (2003) 1121-1131. (Pubitemid 36069121)
14. T.M. Sun, J.Z. Du, L.F. Yan, H.Q. Maod, J. Wang, Self-assembled biodegradable micellar nanoparticles of amphiphilic and cationic block copolymer for siRNA delivery, Biomaterials 29 (2008) 4348-4355.
15. J. Zhu, A. Tang, L.P. Law, M. Feng, K.M. Ho, D.K.L. Lee, F.W. Harris, P. Li, Amphiphilic core-shell nanoparticles with poly(ethylenimine) shells as potential gene delivery carriers, Bioconjugate Chem. 16 (2004) 139-146. (Pubitemid 40129687)
16. Y. Wang, L.S. Wang, S.H. Goh, Y.Y. Yang, Synthesis and characterization of cationic micelles self-assembled from a biodegradable copolymer for gene delivery, Biomacromolecules 8 (2007) 1028-1037. (Pubitemid 46488488)
17. L.Y. Qiu, Y.H. Bae, Self-assembled polyethylenimine-graft-poly(ε- caprolactone) micelles as potential dual carriers of genes and anticancer drugs, Biomaterials 28 (2007) 4132-4142. (Pubitemid 47048375)
18. O. Zelphati, C. Nguyen, M. Ferrari, J. Felgner, Y. Tsai, P.L. Felgner, Stable and monodisperse lipoplex formulations for gene delivery, Gene Ther. 5 (1998) 1272-1282. (Pubitemid 28448437)
19. J. Kim, I. Hwang, D. Britain, T.D. Chung, Y. Sun, D.H. Kim, Microfluidic approaches for gene delivery and gene therapy, Lab Chip 11 (2011) 3941-3948.
20. Y.P. Ho, C.L. Grigsby, F. Zhao, K.W. Leong, Tuning physical properties of nanocomplexes through microfluidics-assisted confinement, Nano Lett. 11 (2011) 2178-2182.
21. J. Clement, K. Kiefer, A. Kimpfler, P. Garidel, R. Peschka-Süss, Large-scale production of lipoplexes with long shelf-life, Eur. J. Pharm. Biopharm. 59 (2005) 35-43. (Pubitemid 39573434)
22. C.G. Koh, X. Kang, Y. Xie, Z. Fei, J. Guan, B. Yu, X. Zhang, L.J. Lee, Delivery of polyethylenimine/DNA complexes assembled in a microfluidics device, Mol. Pharmaceutics 6 (2009) 1333-1342.
23. C.G. Koh, X. Zhang, S. Liu, S. Golan, B. Yu, X. Yang, J. Guan, Y. Jin, Y. Talmon, N. Muthusamy, K.K. Chan, J.C. Byrd, R.J. Lee, G. Marcucci, L.J. Lee, Delivery of antisense oligodeoxyribonucleotide lipopolyplex nanoparticles assembled bymicrofluidic hydrodynamic focusing, J. Control. Release 141 (2010) 62-69.
24. S. Hou, N. Ziebacz, S.A. Wieczorek, E. Kalwarczyk, V. Sashuk, T. Kalwarczyk, T.S. Kaminski, R. Holyst, Formation and structure of PEI/DNA complexes: quantitative analysis, Soft Matter 7 (2011) 6967-6972.
25. T.D. Perrine, W.R. Landis, Analysis of polyethylenimine by spectrophotometry of its copper chelate, J. Polym. Sci., Part A-1: Polym. Chem. 5 (1967) 1993-2003.
26. M. Beck-Broichsitter, E. Rytting, T. Lebhardt, X. Wang, T. Kissel, Preparation of nanoparticles by solvent displacement for drug delivery: a shift in the "ouzo region" upon drug loading, Eur. J. Pharm. Sci. 41 (2010) 244-253.
27. M. Chen, X. Liu, A. Fahr, Skin penetration and deposition of carboxyfluorescein and temoporfin from different lipid vesicular systems: in vitro study with finite and infinite dosage application, Int. J. Pharm. 408 (2011) 223-234.
28. O.M. Merkel, M.A. Mintzer, D. Librizzi, O. Samsonova, T. Dicke, B. Sproat, H. Garn, P.J. Barth, E.E. Simanek, T. Kissel, Triazine dendrimers as nonviral vectors for in vitro and in vivo RNAi: the effects of peripheral groups and core structure on biological activity, Mol. Pharmaceutics 7 (2010) 969-983.
29. Y. Wang, K. Kimura, P.L. Dubin, W. Jaeger, Polyelectrolyte-micelle coacervation: effects of micelle surface charge density, polymer molecular weight, and polymer/surfactant ratio, Macromolecules 33 (2000) 3324-3331 (Washington, DC, United States).
30. F. Bordi, C. Cametti, S. Sennato, M. Diociaiuti, Direct evidence of multicompartment aggregates in polyelectrolyte-charged liposome complexes, Biophys. J. 91 (2006) 1513-1520. (Pubitemid 44174268)
31. V. Milkova, K. Kamburova, I. Petkanchin, T. Radeva, Complexation of ferric oxide particles with pectins of different charge density, Langmuir 24 (2008) 9495-9499.
32. D. Truzzolillo, F. Bordi, F. Sciortino, S. Sennato, Interaction between like-charged polyelectrolyte-colloid complexes in electrolyte solutions: a Monte Carlo simulation study in the Debye-Hückel approximation, J. Chem. Phys. 133 (2010) 024901.
33. Y. Liu, O. Samsonova, B. Sproat, O. Merkel, T. Kissel, Biophysical characterization of hyper-branched polyethylenimine-graft- polycaprolactone- block-mono-methoxyl-poly(ethylene glycol) copolymers (hy-PEI-PCL-mPEG) for siRNA delivery, J. Control. Release 153 (2011) 262-268.
34. T. Riley, T. Govender, S. Stolnik, C.D. Xiong, M.C. Garnett, L. Illum, S.S. Davis, Colloidal stability and drug incorporation aspects of micellar-like PLA-PEG nanoparticles, Colloids Surf., B 16 (1999) 147-159.
35. M. Benfer, R. Reul, T. Betz, T. Kissel, Folic acid-decorated nanocomposites prepared by a simple solvent displacement method, Macromol. Biosci. 12 (2012) 438-445.
36. O.M. Merkel, M. Zheng, M.A. Mintzer, G.M. Pavan, D. Librizzi, M. Maly, H. Höffken, A. Danani, E.E. Simanek, T. Kissel, Molecular modeling and in vivo imaging can identify successful flexible triazine dendrimer-based siRNA delivery systems, J. Control. Release 153 (2011) 23-33.
37. M. Ogris, P. Steinlein, M. Kursa, K. Mechtler, R. Kircheis, E. Wagner, The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells, Gene Ther. 5 (1998) 1425-1433. (Pubitemid 28482671)
38. S.W. Choi, I.W. Cheong, J.H. Kim, Y. Xia, Preparation of uniform microspheres using a simple fluidic device and their crystallization into close-packed lattices, Small 5 (2009) 454-459.
39. H. Kuramoto, Y.S. Park, N. Kaji, M. Tokeshi, K. Kogure, Y. Shinohara, H. Harashima, Y. Baba, On-chip fabrication of multifunctional envelope-type nanodevices for gene delivery, Anal. Bioanal. Chem. 391 (2008) 2729-2733.