Recombinant Aeromonas hydrophila outer membrane protein 48 (Omp48) induces a protective immune response against Aeromonas hydrophila and Edwardsiella tarda

Research in Microbiology

Volumn 163, Issue 4, 2012, Pages 286-291

  Khushiramani, Rekha Matlani ^a   Maiti, Biswajit ^a   Shekar, Malathi ^a   Girisha, Shivani Kallappa ^a   Akash, Naviledasappa ^a   Deepanjali, Appunni ^a   Karunasagar, Iddya ^a   Karunasagar, Indrani ^a  

^a University   (India)

Author keywords

Aeromonas hydrophila; Edwardsiella tarda; Immune response; Labeo rohita; Omp48; Outer membrane protein

Indexed keywords

AMINO ACID; OUTER MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN 48; PORIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AEROMONAS HYDROPHILA; AEROMONAS SALMONICIDA; AEROMONAS VERONII; ARTICLE; EDWARDSIELLA TARDA; FISH; FISH DISEASE; IMMUNE RESPONSE; IMMUNIZATION; IMMUNOGENICITY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SERUM; SURVIVAL; VIBRIO PARAHAEMOLYTICUS; WESTERN BLOTTING;

AEROMONAS HYDROPHILA; ANIMALS; BACTERIAL OUTER MEMBRANE PROTEINS; CARPS; EDWARDSIELLA TARDA; FISH DISEASES; GRAM-NEGATIVE BACTERIAL INFECTIONS; MOLECULAR SEQUENCE DATA; PHYLOGENY; RABBITS; RECOMBINANT PROTEINS;

AEROMONAS HYDROPHILA; AEROMONAS SALMONICIDA; AEROMONAS SOBRIA; AEROMONAS VERONII; BACTERIA (MICROORGANISMS); EDWARDSIELLA TARDA; LABEO ROHITA; ORYCTOLAGUS CUNICULUS; VIBRIO PARAHAEMOLYTICUS;

EID: 84861934158     PISSN: 09232508     EISSN: 17697123     Source Type: Journal    
DOI: 10.1016/j.resmic.2012.03.001     Document Type: Article

References (45)

1. Abbott S.L., Cheung W.K.W., Janda J.M. The genus Aeromonas: biochemical characteristics, atypical reactions and phenotypic identification schemes. J. Clin. Microbiol. 2003, 41:2348-2357.
2. Altwegg M., Geiss H.K. Aeromonas as a human pathogen. Crit. Rev. Microbiol. 1989, 16:253-286.
3. Amend D.F. Potency testing of fish vaccines. Dev. Biol. Stand. 1981, 49:447-454.
4. Ansary A., Haneef R.M., Torres J.L., Yadav M. Plasmids and antibiotics resistance in Aeromonas hydrophila isolated in Malaysia from healthy and diseased fish. J. Fish Dis. 1992, 15:191-196.
5. American Public Health Association (APHA) Compendium of Methods for the Microbiological Examination of Foods 1984, Washington, DC. second ed.
6. Austin B., Austin D.A. Bacterial Fish Pathogens: Disease in Farmed and Wild Fish 1999, Springer- Verlag, New York.
7. Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology 1995, John Wiley and Sons, New York. third ed. F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, K. Struhl (Eds.).
8. Bakopoulos V., Adams A., Richards R.H. The effect of iron limitation growth conditions on the cell and extra cellular components of the fish pathogen Pasteurella piscicida. J. Fish Dis. 1997, 20:297-305.
9. Dodsworth S., Rowland G., Bennett A., Coleman G. Antigenicity of the Aeromonas salmonicida maltose-inducible outer membrane porin (maltoporin) and a hybrid protein derived from it. Biochem. Soc. Trans. 1995, 21:338S.
10. Engvall E., Perlman P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 1971, 8:871-874.
11. Fang H.M., Ling K.C., Ge R., Sin Y.M. Enhancement of protective immunity in blue gourami, Trichogaster trichopterus (Pallas) against Aeromonas hydrophila and Vibrio anguillarum by A. hydrophila major adhesin. J. Fish Dis. 2000, 23:137-145.
12. Guan R., Xiong J., Huang W., Guo S. Enhancement of protective immunity in European eel (Anguilla anguilla) against Aeromonas hydrophila and Aeromonas sobria by a recombinant Aeromonas outer membrane protein. Acta Biochim. Biophys. Sin. 2011, 43:79-88.
13. Hirst I.D., Ellis A.E. Iron regulated Omps of Aeromonas salmonicida are important protective antigens in Atlantic salmon against furunculosis. Fish Shellfish Immunol. 1994, 4:29-45.
14. Jeanteur D., Gletsu N., Pattus F., Buckley J.T. Purification of Aeromonas hydrophila major outer membrane proteins: N-terminal sequence analysis and channel forming properties. Mol. Microbiol. 1992, 6:3355-3356.
15. Karunasagar I., Rosalind G., Karunasagar I. Immunological response of Indian major carps to Aeromonas hydrophila vaccine. J. Fish Dis. 1991, 14:413-417.
16. Karunasagar I., Pai R., Malathi G.R., Karunasagar I. Mass mortality of Penaeus monodon due to antibiotic resistant Vibrio harvey infection. Aquaculture 1994, 128:203-209.
17. Karunasagar I., Ali A., Otta S.K., Karunasagar I. Immunization with bacterial antigens: infections with motile aeromonads. Dev. Biol. Stand. 1997, 90:135-141.
18. Kawai K., Liu Y., Ohnishi Y.L., Oshima S. A conserved 37 kDa outer membrane protein of Edwardsiella tarda is an effective vaccine candidate. Vaccine 2004, 22:3411-3418.
19. Khushiramani R., Girisha S.K., Karunasagar I., Karunasagar I. Cloning and expression of an outer membrane protein ompTS of Aeromonas hydrophila and study of immunogenicity in fish. Protein Expr. Purif. 2007, 51:303-307.
20. Khushiramani R., Girisha S.K., Karunasagar I., Karunasagar I. Protective efficacy of recombinant OmpTS protein of Aeromonas hydrophila in Indian major carp. Vaccine 2007, 26:157-1158.
21. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
22. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 1951, 193:265-273.
23. Lutwyche P., Exner M.M., Hancock R.E., Trust T.J. A conserved Aeromonas salmonicida porin provides protective immunity to rainbow trout. Infect. Immun. 1995, 63:3137-3142.
24. Maiti B., Raghunath P., Karunasagar I., Karunasagar I. Cloning and expression of an outer membrane protein OmpW of Aeromonas hydrophila and study of its distribution in Aeromonas spp. J. Appl. Microbiol. 2009, 107:1157-1167.
25. Maiti B., Shetty M., Shekar M., Karunasagar I., Karunasagar I. Protective immunity of recombinant outer membrane protein A (OmpA) of Edwardsiella tarda for fish, common carp. Microbiol. Res. 2011, 167:1-7.
26. McIntyre S., Eschbach M.L., Mutschler B. Export incompatibility of N- terminal basic residues in a mature polypeptide of Escherichia coli can be alleviated by optimizing of the signal peptide. Mol. Genet. Genomics 1990, 221:466-474.
27. Newman S.G. Bacterial vaccines for fish. Ann. Rev. Fish Dis. 1993, 3:145-185.
28. Ni X.D., Wang N., Liu Y.J., Lu C.P. Immunoproteomics of extracellular proteins of the Aeromonas hydrophila China vaccine strain J-1 reveal a highly immunoreactive outer membrane protein. FEMS Immunol. Med. Microbiol. 2010, 58:363-373.
29. Nikaido H. Porins and specific channels of bacterial outer membranes. Mol. Microbiol. 1992, 6:435-442.
30. Nogueras M.M., Merino S., Aguilar A., Benedi V.J., Tomas J.M. Cloning, sequencing and role in serum susceptibility of porin II from mesophilic Aeromonas hydrophila. Infect. Immun. 2000, 68:1849-1854.
31. Quinn D.M., Atkinson H.M., Bretag A.H., Tester M., Trust T.J., Wong C.Y., Flower R.L. Carbohydrate-reactive, pore-forming outer membrane proteins of Aeromonas hydrophila. Infect. Immun. 1994, 62:4054-4058.
32. Rahman M.H., Kawai K. Outer membrane proteins of Aeromonas hydrophila induce protective immunity in goldfish. Fish Shellfish Immunol. 2000, 10:379-382.
33. Sachadeva G., Kumar K., Jain P., Ramachandran S. SPAAN: a software for prediction of adhesions and adhesin-like proteins using neural networks. Bioinformatics 2005, 21:483-491.
34. Sakai T., Matsuyama T., Nishioka T., Nakayasu C., Kamaishi T., Yamaguchi K., Iida T. Identification of major antigenic proteins of Edwardsiella tarda recognized by Japanese flounder antibody. J. Vet. Diagn. Invest. 2009, 21:504-509.
35. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning, A Laboratory Manual 1989, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. second ed.
36. Tamura K., Sakazaki R., McWhorter A.C., Kosako Y. Edwardsiella tarda serotyping scheme for international use. J. Clin. Microbiol. 1988, 26:2343-2346.
37. Tang X., Zhan W., Sheng X., Chi H. Immune response of Japanese flounder Paralichthys olivaceus to outer membrane protein of Edwardsiella tarda. Fish Shellfish Immunol. 2010, 28:333-343.
38. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U S A 1979, 76:4350-4354.
39. Vazquez-Juarez R.C., Barrera-Saldana H.A., Hernandez-Saavedra N.Y., Gomez-Chiarri M., Ascencio F. Molecular cloning, sequencing and characterization of omp48, the gene encoding for an antigenic outer membrane protein from Aeromonas veronii. J. Appl. Microbiol. 2003, 94:908-918.
40. Vazquez-Juarez R.C., Romero M.J., Ascencio F. Adhesive properties of a LamB-like outer-membrane protein and its contribution to Aeromonas veronii adhesion. J. Appl. Microbiol. 2004, 96:700-708.
41. Vazquez-Juarez R.C., Gomez-Chiarri M., Barrera-Saldana H., Hernmandez-Saaverda N., Dumas S., Ascencio F. Evaluation of DNA vacation of spotted sand bass (Paralabrax maculatofasciatus) with two major outer membrane protein encoding genes from Aeromonas veronii. Fish Shellfish Immunol. 2005, 19:153-163.
42. Von Heijnem G. Signal sequence: the limits of variation. J. Mol. Biol. 1985, 184:99-105.
43. Wang C., Liu Y., Li H., Xu W.J., Zhang H., Peng X.X. Identification of plasma-responsive outer membrane proteins and their vaccine potential in Edwardsiella tarda using proteomic approach. J. Proteomics 2012, 75:1263-1275.
44. Wimley W.C. Toward genomic identification of beta-barrel membrane proteins: composition and architecture of known structures. Protein Sci. 2002, 11:301-312.
45. Yanez M.A., Catalan V., Apraiz D., Figureras M.J., Martinez-Murcia A.J. Phylogenetic analysis of members of the genus Aeromonas based on gyrB gene sequences. Int. J. Syst. Evol. Microbiol. 2003, 53:875-883.