Sensitive method for determination of protein and cell concentrations based on competitive adsorption to nanoparticles and time-resolved luminescence resonance energy transfer between labeled proteins

Analytical Chemistry

Volumn 84, Issue 11, 2012, Pages 4950-4956

  Pihlasalo, Sari ^a   Puumala, Pauli ^a   Hänninen, Pekka ^a   Härmä, Harri ^a  

^a UNIVERSITY OF TURKU   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTES; CELL CONCENTRATIONS; CELL COUNTING; CLOSE PROXIMITY; COEFFICIENT OF VARIATION; COMPETITIVE ADSORPTION; DETECTION LIMITS; DETERMINATION OF PROTEINS; EUKARYOTIC CELLS; LABELED PROTEINS; OPTIMIZED CONDITIONS; POLYSTYRENE NANOPARTICLES; RESONANCE ENERGY TRANSFER; SENSITIVE METHOD; TIME-RESOLVED LUMINESCENCE;

ADSORPTION; ASSAYS; CARBOXYLATION; ENERGY TRANSFER; NANOPARTICLES; POLYSTYRENES;

PROTEINS;

BOVINE SERUM ALBUMIN; BUFFER; EUROPIUM; FLUORESCENT DYE; NANOPARTICLE; POLYSTYRENE DERIVATIVE;

ADSORPTION; ANIMAL; ARTICLE; CATTLE; CELL COUNT; CHEMISTRY; CHO CELL; FLUORESCENCE RESONANCE ENERGY TRANSFER; HAMSTER; KINETICS; LUMINESCENCE; METHODOLOGY; PH; REPRODUCIBILITY;

ADSORPTION; ANIMALS; BUFFERS; CATTLE; CELL COUNT; CHO CELLS; CRICETINAE; EUROPIUM; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; KINETICS; LUMINESCENCE; NANOPARTICLES; POLYSTYRENES; REPRODUCIBILITY OF RESULTS; SERUM ALBUMIN, BOVINE;

EID: 84861913536     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac300597j     Document Type: Article

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