Cell-free protein synthesis inside giant unilamellar vesicles analyzed by flow cytometry

Langmuir

Volumn 28, Issue 22, 2012, Pages 8426-8432

  Nishimura, Koji ^a   Matsuura, Tomoaki ^a,b   Nishimura, Kazuya ^a   Sunami, Takeshi ^b   Suzuki, Hiroaki ^a,b   Yomo, Tetsuya ^a,b  

^a OSAKA UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANIONIC PHOSPHOLIPIDS; BIOCHEMICAL REACTIONS; CELL FREE PROTEIN SYNTHESIS; CELL SYSTEM; CHEMICAL COMPOSITIONS; GIANT UNILAMELLAR VESICLES; GREEN FLUORESCENT PROTEIN; IN-VITRO; LIPID MEMBRANES; LIPID VESICLES; MULTI-LAMELLAR VESICLES; PHOSPHOLIPID MEMBRANE; PROTEIN SYNTHESIS; REACTION VOLUME; TEST TUBE; TIME COURSE;

BIOLOGICAL MEMBRANES; FLOW CYTOMETRY; PHOSPHOLIPIDS;

BIOSYNTHESIS;

CHOLESTEROL; GREEN FLUORESCENT PROTEIN; LIPOSOME; PHOSPHATIDYLCHOLINE;

ARTICLE; BIOSYNTHESIS; CELL FREE SYSTEM; CHEMISTRY; DRUG EFFECT; DRUG FORMULATION; FLOW CYTOMETRY; KINETICS; METABOLISM; PARTICLE SIZE; PROTEIN SYNTHESIS;

CELL-FREE SYSTEM; CHOLESTEROL; DRUG COMPOUNDING; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; KINETICS; PARTICLE SIZE; PHOSPHATIDYLCHOLINES; PROTEIN BIOSYNTHESIS; UNILAMELLAR LIPOSOMES;

EID: 84861861133     PISSN: 07437463     EISSN: 15205827     Source Type: Journal    
DOI: 10.1021/la3001703     Document Type: Article

References (38)

1. Goodsell, D. S. The Machinery of Life, 2 nd ed.; Copernicus Books: New York, 2009.
2. Zhou, H. X.; Rivas, G.; Minton, A. P. Macromolecular crowding and confinement: biochemical, biophysical, and potential physiological consequences Annu. Rev. Biophys. 2008, 37, 375-97
3. Minton, A. P. How can biochemical reactions within cells differ from those in test tubes? J. Cell Sci. 2006, 119 (Pt 14) 2863-9
4. Kato, A.; Shindo, E.; Sakaue, T.; Tsuji, A.; Yoshikawa, K. Conformational transition of giant DNA in a confined space surrounded by a phospholipid membrane Biophys. J. 2009, 97 (6) 1678-86
5. Tsuji, A.; Yoshikawa, K. ON-OFF switching of transcriptional activity of large DNA through a conformational transition in cooperation with phospholipid membrane J. Am. Chem. Soc. 2010, 132 (35) 12464-71
6. Walde, P.; Ichikawa, S. Enzymes inside lipid vesicles: preparation, reactivity and applications Biomol. Eng. 2001, 18 (4) 143-77
7. Sunami, T.; Kita, H.; Hosoda, K.; Matsuura, T.; Suzuki, H.; Yomo, T., Chapter 2-Detection and analysis of protein synthesis and RNA replication in giant liposomes. Methods Enzymol. 2009, 464, 19-30.
8. Sunami, T.; Sato, K.; Matsuura, T.; Tsukada, K.; Urabe, I.; Yomo, T. Femtoliter compartment in liposomes for in vitro selection of proteins Anal. Biochem. 2006, 357 (1) 128-36
9. Hosoda, K.; Sunami, T.; Kazuta, Y.; Matsuura, T.; Suzuki, H.; Yomo, T. Quantitative study of the structure of multilamellar giant liposomes as a container of protein synthesis reaction Langmuir 2008, 24 (23) 13540-8
10. Kita, H.; Matsuura, T.; Sunami, T.; Hosoda, K.; Ichihashi, N.; Tsukada, K.; Urabe, I.; Yomo, T. Replication of genetic information with self-encoded replicase in liposomes ChemBioChem 2008, 9 (15) 2403-10
11. Sunami, T.; Hosoda, K.; Suzuki, H.; Matsuura, T.; Yomo, T. Cellular compartment model for exploring the effect of the lipidic membrane on the kinetics of encapsulated biochemical reactions Langmuir 2010, 26 (11) 8544-51
12. Ishikawa, K.; Sato, K.; Shima, Y.; Urabe, I.; Yomo, T. Expression of a cascading genetic network within liposomes FEBS Lett. 2004, 576 (3) 387-90
13. Nomura, S. M.; Tsumoto, K.; Hamada, T.; Akiyoshi, K.; Nakatani, Y.; Yoshikawa, K. Gene expression within cell-sized lipid vesicles ChemBioChem 2003, 4 (11) 1172-5
14. Yamaji, K.; Kanai, T.; Nomura, S. M.; Akiyoshi, K.; Negishi, M.; Chen, Y.; Atomi, H.; Yoshikawa, K.; Imanaka, T. Protein synthesis in giant liposomes using the in vitro translation system of Thermococcus kodakaraensis IEEE Trans. Nanobiosci. 2009, 8 (4) 325-31
15. Pereira de Souza, T.; Stano, P.; Luisi, P. L. The minimal size of liposome-based model cells brings about a remarkably enhanced entrapment and protein synthesis ChemBioChem 2009, 10 (6) 1056-63
16. Saito, H.; Kato, Y.; Le Berre, M.; Yamada, A.; Inoue, T.; Yosikawa, K.; Baigl, D. Time-resolved tracking of a minimum gene expression system reconstituted in giant liposomes ChemBioChem 2009, 10 (10) 1640-3
17. Amidi, M.; de Raad, M.; de Graauw, H.; van Ditmarsch, D.; Hennink, W. E.; Crommelin, D. J.; Mastrobattista, E. Optimization and quantification of protein synthesis inside liposomes J. Liposome Res. 2010, 20 (1) 73-83
18. Noireaux, V.; Libchaber, A. A vesicle bioreactor as a step toward an artificial cell assembly Proc. Natl. Acad. Sci. U. S. A. 2004, 101 (51) 17669-74
19. Pereira de Souza, T.; Steiniger, F.; Stano, P.; Fahr, A.; Luisi, P. L. Spontaneous Crowding of Ribosomes and Proteins inside Vesicles: A Possible Mechanism for the Origin of Cell Metabolism. ChemBioChem 2011, not supplied.
20. Luisi, P. L.; Allegretti, M.; Pereira de Souza, T.; Steiniger, F.; Fahr, A.; Stano, P. Spontaneous protein crowding in liposomes: a new vista for the origin of cellular metabolism ChemBioChem 2010, 11 (14) 1989-92
21. Nishimura, K.; Hosoi, T.; Sunami, T.; Toyota, T.; Fujinami, M.; Oguma, K.; Matsuura, T.; Suzuki, H.; Yomo, T. Population analysis of structural properties of giant liposomes by flow cytometry Langmuir 2009, 25 (18) 10439-43
22. Murtas, G.; Kuruma, Y.; Bianchini, P.; Diaspro, A.; Luisi, P. L. Protein synthesis in liposomes with a minimal set of enzymes Biochem. Biophys. Res. Commun. 2007, 363 (1) 12-7
23. Pautot, S.; Frisken, B. J.; Weitz, D. A. Production of Unilamellar Vesicles Using an Inverted Emulsion Langmuir 2003, 19 (7) 2870-2879
24. Sunami, T.; Matsuura, T.; Suzuki, H.; Yomo, T. Synthesis of functional proteins within liposomes Methods Mol. Biol. 2010, 607, 243-56
25. Matsuura, T.; Ichihashi, N.; Sunami, T.; Kita, H.; Suzuki, H.; Yomo, T. Evolvability and Self-Replication of Genetic Information in Liposomes. In The Minimal Cell; Luisi, P. L.; Stano, P., Eds.; Springer: Dordrecht, 2011; pp 275-287.
26. Shimizu, Y.; Inoue, A.; Tomari, Y.; Suzuki, T.; Yokogawa, T.; Nishikawa, K.; Ueda, T. Cell-free translation reconstituted with purified components Nat. Biotechnol. 2001, 19 (8) 751-5
27. Shimizu, Y.; Kanamori, T.; Ueda, T. Protein synthesis by pure translation systems Methods 2005, 36 (3) 299-304
28. Ito, Y.; Suzuki, M.; Husimi, Y. A novel mutant of green fluorescent protein with enhanced sensitivity for microanalysis at 488 nm excitation Biochem. Biophys. Res. Commun. 1999, 264 (2) 556-60
29. Takiguchi, K.; Yamada, A.; Negishi, M.; Tanaka-Takiguchi, Y.; Yoshikawa, K. Entrapping desired amounts of actin filaments and molecular motor proteins in giant liposomes Langmuir 2008, 24 (20) 11323-6
30. Umakoshi, H.; Tanabe, T.; Suga, K.; Bui, H. T.; Shimanouchi, T.; Kuboi, R. Oxidative stress can affect the gene silencing effect of DOTAP liposome in an in vitro translation system Int. J. Biol. Sci. 2011, 7 (3) 253-60
31. Umakoshi, H.; Suga, K.; Bui, H. T.; Nishida, M.; Shimanouchi, T.; Kuboi, R. Charged liposome affects the translation and folding steps of in vitro expression of green fluorescent protein J. Biosci. Bioeng. 2009, 108 (5) 450-4
32. Bui, H. T.; Umakoshi, H.; Ngo, K. X.; Nishida, M.; Shimanouchi, T.; Kuboi, R. Liposome membrane itself can affect gene expression in the Escherichia coli cell-free translation system Langmuir 2008, 24 (19) 10537-42
33. Caschera, F.; Sunami, T.; Matsuura, T.; Suzuki, H.; Hanczyc, M. M.; Yomo, T. Programmed Vesicle Fusion Triggers Gene Expression. Langmuir 2011, not supplied.
34. Finkelstein, A. Water Movement through Lipid Bilayers, Pores, and Plasma Membranes: Theory and Reality; Wiley: New York, 1987; p xii, 228 pp.
35. Matsuura, T.; Kazuta, Y.; Aita, T.; Adachi, J.; Yomo, T. Quantifying epistatic interactions among the components constituting the protein translation system Mol. Syst. Biol. 2009, 5, 297
36. Mansy, S. S.; Schrum, J. P.; Krishnamurthy, M.; Tobe, S.; Treco, D. A.; Szostak, J. W. Template-directed synthesis of a genetic polymer in a model protocell Nature 2008, 454 (7200) 122-5
37. Monnard, P. A.; Luptak, A.; Deamer, D. W. Models of primitive cellular life: polymerases and templates in liposomes Philos. Trans. R. Soc., B 2007, 362 (1486) 1741-50
38. Walde, P.; Goto, A.; Monnard, P.-A.; Wessicken, M.; Luisi, P. Oparin's reaction revisited: enzymatic synthesis of poly(adenyl acid) in micelles and self-reproducing vesicles J. Am. Chem. Soc. 1994, 116, 7541-7547